Engineering Rubisco in Higher Plant Chloroplasts
Attempts to alter the characteristics of higher plant Rubisco by applying the usual molecular biological procedures of construction and over-production from plasmids in the usual bacterial hosts is fraught with difficulties. A major problem, so far not surmounted is the inability of the recombinant protein to assemble into any aggregate that remotely resembles a competent functioning entity. Most examples of successful manipulation of an L8S8 form of the enzyme in E.coli have been forthcoming using the dicistronic gene of cyanobacteria (see refs cited in 1). Even plant — cyanobacteria chimeric constructions resulted in only limited success in recovering active enzyme.
Keywordschloroplast transformation Rubisco
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