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Engineering Rubisco in Higher Plant Chloroplasts

  • Ivan Kanevski
  • Pal Maliga
  • Dan Rhoades
  • Steven Gutteridge
Chapter

Abstract

Attempts to alter the characteristics of higher plant Rubisco by applying the usual molecular biological procedures of construction and over-production from plasmids in the usual bacterial hosts is fraught with difficulties. A major problem, so far not surmounted is the inability of the recombinant protein to assemble into any aggregate that remotely resembles a competent functioning entity. Most examples of successful manipulation of an L8S8 form of the enzyme in E.coli have been forthcoming using the dicistronic gene of cyanobacteria (see refs cited in 1). Even plant — cyanobacteria chimeric constructions resulted in only limited success in recovering active enzyme.

Keywords

chloroplast transformation Rubisco 

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References

  1. 1.
    Gutteridge S, Gatenby A. A. (1995) Plant Cell 7: 809–819CrossRefPubMedPubMedCentralGoogle Scholar
  2. 2.
    Svab Z, Maliga P (1993) Proc Natl Acad Sci USA 90: 913–917CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    Rochaix J-D (1997) Trends in Pl Sei 2: 419–425CrossRefGoogle Scholar
  4. 4.
    Kanevski I, Maliga P (1994) Proc Natl Acad Sci. USA 91: 1969–1973CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 1998

Authors and Affiliations

  • Ivan Kanevski
    • 1
    • 2
  • Pal Maliga
    • 1
    • 2
  • Dan Rhoades
    • 1
    • 2
  • Steven Gutteridge
    • 1
    • 2
  1. 1.Waksman Institute, RutgersState University of New JerseyPiscatawayUSA
  2. 2.CR&D DepartmentDuPont CompanyUSA

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