Skip to main content
Book cover

Photosynthesis: Mechanisms and Effects pp 1827–1830Cite as

Effect of Cholesterol and Benzyl Alcohol on Prompt and Delayed Chlorophyll Fluorescence in Thylakoid Membranes

  • Chapter

Abstract

Photosynthesis is one of most sensitive processes determining plant response to environmental stress. Possible stress-induced reaction is modification of fluidity of the chloroplast thylakoid membrane (TM). The TM physical state may plays an important role in controlling of the light reactions of photosynthesis (1 – 3). Artificial alteration of the TM fluidity reflects on the whole chain electron transport rate, proton uptake, oxidation of the primary electron acceptor of PS II (QA), cytochrome f reduction and parameters of cation-induced chlorophyll fluorescence (3). Cholesterol incorporation leads to a decrease of membrane fluidity, while the treatment with benzyl alcohol has opposite effect (4). The effect of both membrane perturbing agents on the fluorescent transients of pea thylakoids expressed as follows: a decrease of PS IIα centers and of the Fv/Fm ratio in cholesterol-treated TM; in benzyl alcohol-treated TM the chlorophyll a fluorescence induction parameters are influenced to a smaller extent. The level of QB-nonreducing centers seems not to be altered by either treatment (5). The artificial TM fluidity alteration would be reflected also on other luminescence characteristics of photosynthetic apparatus.

Key words

  • Chl fluorescence induction
  • electron transport
  • energy transfer
  • membrane fluidity
  • membrane perturbing agents
  • photosystem 2

This is a preview of subscription content, access via your institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • DOI: 10.1007/978-94-011-3953-3_427
  • Chapter length: 4 pages
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
eBook
USD   949.00
Price excludes VAT (USA)
  • ISBN: 978-94-011-3953-3
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book
USD   1,199.99
Price excludes VAT (USA)
Hardcover Book
USD   1,799.99
Price excludes VAT (USA)
Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Quinn, P.J. and Williams, W.P. (1978) Prog. Biophys. Mol. Biol. 34, 109–173

    CrossRef  CAS  PubMed  Google Scholar 

  2. Yamamoto, Y., Ford, R.C. and Barber, J. (1982) Plant Physiol., 1069–1072

    Google Scholar 

  3. Pedersen, J. and Cox, R. (1984) Advances in Photosynth. Res. vol. 3, 51–54

    CAS  Google Scholar 

  4. Dobrikova, A., Tuparev, N., Krasteva, I., Busheva, M. and Velitchkova, M. (1997) Z. Naturfosch. 52, 475–480

    CAS  Google Scholar 

  5. Busheva, M., Velitchkova, M., Markova, Tz. and Zanev, Y. (1998) J. Photochem. Photobiol B, 42, 240–244

    CrossRef  CAS  Google Scholar 

  6. Lavorel, J. (1975) In Bioenergetics of Photosynthesis (Govindjee, ed.) pp. 223–317, Academic Press, New York, San Francisco, London

    CrossRef  Google Scholar 

  7. Goltsev, V. and Yordanov, I. (1997) Photosynthetica 33 (3–4), 571–586

    CAS  Google Scholar 

  8. Krause, G.H. and Weis, E. (1991) Annu. Rev. Plant Physiol. 42, 313–349

    CrossRef  CAS  Google Scholar 

  9. Radenovich, C., Markovic, D. and Jeremic, M. (1994) Photosynthetica, 30, 1–2

    Google Scholar 

  10. Whatley, F.R. and Arnon, D.I. (1963) In Methods in Enzimology (Colowick S.P. and Kaplan NO., eds.) v. 6, pp. 308–313, Academic Press, New York

    Google Scholar 

  11. Lichtenthaler, H.K. (1987) Methods Enzymol. 148, 350–382

    CrossRef  CAS  Google Scholar 

  12. Goldfeld, M.G., Mikojan, V.D., Suskina, V.I., Timofeev, V.P., Shapiro, A.B. (1980) Plant Physiol. (Moscow) 27, 1143–1153 (in Russian)

    CAS  Google Scholar 

  13. Ford, R.C. and Barber, J. (1980) Photobiochem.Photobiophys. 1, 263–270

    CAS  Google Scholar 

  14. Wraight C.A. and Crofts, A.R. (1971) Eur. J. Biochem. 19, 386–397

    CrossRef  CAS  PubMed  Google Scholar 

  15. Ford, R.C. and Barber, J. (1983) Planta 158, 35–41

    CrossRef  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Dept. Biophysics and Radiobiology, Faculty of Biology, University of Sofia, Bulgaria

    I. Zaharieva & V. Goltsev

  2. Institute of Biophysics, BAS, Acad. G. Bonchev str. bl. 21, Sofia, Bulgaria

    M. Velitchkova

Authors
  1. I. Zaharieva
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Velitchkova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. V. Goltsev
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary

    G. Garab

Rights and permissions

Reprints and Permissions

Copyright information

© 1998 Springer Science+Business Media Dordrecht

About this chapter

Cite this chapter

Zaharieva, I., Velitchkova, M., Goltsev, V. (1998). Effect of Cholesterol and Benzyl Alcohol on Prompt and Delayed Chlorophyll Fluorescence in Thylakoid Membranes. In: Garab, G. (eds) Photosynthesis: Mechanisms and Effects. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3953-3_427

Download citation

  • DOI: https://doi.org/10.1007/978-94-011-3953-3_427

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-0-7923-5547-2

  • Online ISBN: 978-94-011-3953-3

  • eBook Packages: Springer Book Archive