To an Optimal Design of an Airlift Bioreactor for the Cultivation of Hybridomas

  • D. E. Martens
  • Martin J. Coco
  • Cam van der Velden-De Groot
  • E. C. Beuvery
  • C. D. de Gooijer
  • J. Tramper
Part of the Developments in Biotherapy book series (DIBI, volume 1)

Abstract

One of the main problems in scaling up hybridoma cell cultures is the supply of oxygen, since sparging and agitation can lead to excessive cell death. This study concerns the relation between the air flow and cell death in an airlift bioreactor. In addition we studied the effects of lowering the serum concentration on cell density and viability, on the productivity of the cells, on the concentrations of glucose, lactate and ammonia, on the LDH activity, on the oxygen consumption rate and on the shear sensitivity of the hybridoma cells.

It appears that the death rate of the cells is linearly proportional to the air flow and that the hybridoma cells become more shear sensitive at lower serum concentrations in the range from 5% to 2%. In this range serum concentration did not show any major effect upon the cell density and viability, the productivity, the concentrations of glucose, lactate and ammonia, LDH activity, nor upon the oxygen consumption rate.

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References

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    Tramper J, Smit D, Straatman J, Vlak JM (1988). Bubble column design for growth of fragile insect cells. Bioproc. Eng. 3. 37–41.CrossRefGoogle Scholar
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    Oh SKW, Nienow AW, Al-Rubeai M, Emery AN (1989). The effects of agitation intensity with and without continuous sparging on the growth and antibody production of hybridoma cells. J.Biotechnol. 12: 45–62.CrossRefGoogle Scholar
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    Handa A (1986). Gas liquid interfacial effects on the growth of hybridomas and other suspended mammalian cells. Thesis, University of Birmingham.Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 1990

Authors and Affiliations

  • D. E. Martens
  • Martin J. Coco
  • Cam van der Velden-De Groot
  • E. C. Beuvery
  • C. D. de Gooijer
  • J. Tramper

There are no affiliations available

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