Abstract
Homopyrimidine oligodeoxynucleotides have been covalently linked to intercalating agents. These bifunctional nucleic acid ligands bind to the major groove of DNA at homopurine-homopyrimidine sequences when they form triple helices. Spectroscopic studies and molecular modelling shows that the intercalating agent inserts its aromatic ring at the triplex-duplex junction. A strong stabilization of triple helical structures is observed. Energy transfer can occur between two derivatized oligonucleotides bound to neighboring sequences on both single-stranded and double-stranded DNA. Bifunctional oligonucleotide-intercalator conjugates provide new tools for a selective control of gene expression and for the detection of close proximity between two sequences on nucleic acids.
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Montenay-Garestier, T. et al. (1990). Design of Bifunctional Nucleic Acid Ligands. In: Pullman, B., Jortner, J. (eds) Molecular Basis of Specificity in Nucleic Acid-Drug Interactions. The Jerusalem Symposia on Quantum Chemistry and Biochemistry, vol 23. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3728-7_19
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DOI: https://doi.org/10.1007/978-94-011-3728-7_19
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