Abstract
With the example of the enzymatically catalyzed hydrolysis of lactose the process optimization of steam-sterilizable dialysis membrane reactors is carried out up to industrial scale.
The expected conversion of lactose in the membrane reactor is dominated by the mass transfer resistance and the real flow in the reactor. Therefore a model for real reactors is developed to describe the transport reaction behaviour of the membrane reactors which considers the non-linear kinetics of the native enzyme, the real mixing conditions in the reactor and the mass transfer over the membrane. The calculation is carried out with the help of a coupled numerical solution.
By experimental investigations the enzyme kinetics, the mass transfer in the membrane, the hydrodynamics and the conversion are measured. The model permits the calculation of important process parameters.
For the hydrolysis of lactose by ß-galactosidase from Kluyveromyces marxianus the theoretical calculations with the developed model show good agreement with experimental results.
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Abbreviations
- A m2 :
-
membrane surface area
- C mol·m-3 :
-
concentration
- di m:
-
inside diameter of hollow fibre
- D m2·s-1 :
-
diffusion coefficient
- Dm m2·s-1 :
-
effective diffusion coefficient in the swollen membrane
- Ea J·mol-1 :
-
activation energy
- ko m·s-1 :
-
mass transfer coefficient
- KM mol·m-3 :
-
Michaelis constant
- KI mol·m-3 :
-
inhibition constant
- L m:
-
length of hollow fibre
- K,N,M:
-
number of tanks
- n:
-
number of hollow fibres
- r mol·s-1 :
-
reaction rate
- t s:
-
time
- ̇ m3·s-1 :
-
volumetric flow rate
- V m3 :
-
volume
- Vmax mol·s-1·m-3 :
-
maximum reaction rate
- X:
-
conversion
- Yp/s :
-
molar yield of product on substrate
- σ2 :
-
variance of residence time related to dimensionless time
- θ:
-
dimensionless time
- Re:
-
Reynolds number
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© 1991 Elsevier Science Publishers Ltd, England
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Czermak, P., Bauer, W.J. (1991). Process Optimization of an Enzyme Membrane Reactor with Soluble Enzymes up to Industrial Scale. In: Turner, M.K. (eds) Effective Industrial Membrane Processes: Benefits and Opportunities. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3682-2_18
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DOI: https://doi.org/10.1007/978-94-011-3682-2_18
Publisher Name: Springer, Dordrecht
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