Preparation and Characteristics of Monoclonal Antibodies Reactive to Satsuma Dwarf Virus
Satsuma Dwarf Virus (SDV) is the first virus disease reported(3) on citrus in Japan. It is now distributed widely in almost all citrus-growing districts in Japan. This virus is a soil-transmissible plant pathogen(1). It is difficult to grow citrus trees on contaminated fields. The detection of the virus before planting is effective in preventing the spread of the disease. Enzyme-linked immunosorbent assay (ELISA) first came into used in 1980(2) for SDV detection. However, it is diffic ult to obtain reproducible results using polyclonal antibodies. We attem ped to produce monoclonal antibodies to SDV.
KeywordsAmmonium Sulfate Sodium Selenite Sucrose Density Gradient Mercaptoacetic Acid Density Gradient Ultracentrifugation
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