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Preparation and Characteristics of Monoclonal Antibodies Reactive to Satsuma Dwarf Virus

  • Keita Hirashima
  • Yasuhiro Noguchi
  • Hiroki Murakami

Abstract

Satsuma Dwarf Virus (SDV) is the first virus disease reported(3) on citrus in Japan. It is now distributed widely in almost all citrus-growing districts in Japan. This virus is a soil-transmissible plant pathogen(1). It is difficult to grow citrus trees on contaminated fields. The detection of the virus before planting is effective in preventing the spread of the disease. Enzyme-linked immunosorbent assay (ELISA) first came into used in 1980(2) for SDV detection. However, it is diffic ult to obtain reproducible results using polyclonal antibodies. We attem ped to produce monoclonal antibodies to SDV.

Keywords

Ammonium Sulfate Sodium Selenite Sucrose Density Gradient Mercaptoacetic Acid Density Gradient Ultracentrifugation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Literature Cited

  1. 1.
    Izawa, H. 1966. Investigations on withering disease of Citrus unshu Marcov. in Gamagori District, Aichi Prefecture. Aichi Hort. Exp. Sta. 5:1–9.Google Scholar
  2. 2.
    Usugi, T. and Saito, Y. 1980. Detection of Satsuma Dwarf Virus by Enzyme-Linked Immunosorubent Assay. Abstr. Ann. Phytopath. soc. Japan 46: 106.Google Scholar
  3. 3.
    Yamada, S., and K. Sawamura. 1950 Satsuma dwarf. Abstr. Proc. Cong. Hort. Soc. Autumun 1950. 36–37.Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 1991

Authors and Affiliations

  • Keita Hirashima
    • 1
  • Yasuhiro Noguchi
    • 1
  • Hiroki Murakami
    • 2
  1. 1.The Branch of Fruit Tree SaplingsFukuoka Agricultural Research CenterTanushimaru-town, Ukiha-gun, FukuokaJapan
  2. 2.Graduate School of Genetic Resources Technology, Faculty of AgricultureKyushu University 46-09Higashi-ku, FukuokaJapan

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