Assay Systems Using Cultured Hepatocytes for Pharmacological and Nutritional Actions of Amino Acids and Related Compounds
Actions of amino acids and related compounds on lipid and protein metabolism were studied in two kinds of rat hepatocytes. In primary monolayer cultures of hepatocytes from rats bearing hepatomas, L-cysteine was found to stimulate bile acid production. A cystineenriched diet reduced in vivo a hepatoma-induced hypercholesterolemia with an enhancement of fecal bile acid excretion. In an established cell line of RLC-16 hepatocytes, L-leucine and its ketoacid derivative were found to stimulate protein synthesis. From inhibitor studies, their stimulatory action on protein synthesis in RLC-16 cells was suggested to be mediated by protein kinase C activation. The cultured hepatocytes used here may be effective for screening the actions of amino acids, and for studying the modes of their actions.
KeywordsBile Acid Stimulatory Action Bile Acid Synthesis Bile Acid Metabolism Stimulate Protein Synthesis
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