Detection of Bovine Viruses by Monoclonal Antibodies
We established sixteen mouse monoclonal antibodies (MAbs) reactive to Chuzan virus K-47 strain using P3-X63-Ag8-U1 cells as fusion partners. Among them, CG53/2/4 recognized a 100K structural protein of the virus. This 100K antigen is a glycoprotein which is essential for the infection of Chuzan virus. The other anti-Chuzan virus MAbs reacted with a 41K antigen of the virus. Especially CGI/1 showed the highest reactivity to the virus. Forward step sandwich assay using CG1/1 and biotinylated CG53/2/4 could detect Chuzan virus at 10 TCID50/ml. Furthermore we established fourteen MAbs reactive to bovine ephemeral fever virus (BEFV) YHL strain. Among them, YM4/9 reacted specifically with a 43K antigen of BEFV, corresponding to the matrix protein 1. The other MAbs reacted not only with the 43K antigen, but also with unknown 23K and 21K antigens. By a simultaneous two-site method using YM4/9 and YG3/4, it was possible to detect 104 . 10TCID50/m1 of BEFV in the presence of bovine serum. Therefore, these MAbs can eventually predict the virus infection of cattle before sideration.
KeywordsHost Cell Line Bovine Ephemeral Fever Virus Sandwich ELISA Method Bovine Virus Akabane Virus
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