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Immunoglobulin Production Stimulation by Various Types of Caseins

  • K. Yamada
  • H. Nakajima
  • L. Ikeda
  • S. Shirahata
  • A. Enomoto
  • S. Kaminogawa
  • H. Murakami

Abstract

α-, ß-, and k caseins are immunoglobulin production stimulating factors (IPSFs), which stimulate proliferation and immunoglobulin production of hybridomas in serum-free media. To localize the proliferation stimulating and IPSF active sites of these caseins, effects of exopeptidase digestions on these activities of caseins were examined. Carboxypeptidase Y and aminopeptidase M digestions of caseins showed that both C- and N-terminals are important for their proliferation stimulating and IPSF activities. When synthesized a-casein peptides were added to human-human hybridoma HB4C5 cells, four peptides (1–20, 16–35, 91–110, and 106125) stimulated their proliferation, but no peptide showed IPSF activity. When the peptides were added to human lymphocyte cultures in the presence of lipopolysaccharides, four peptides belonging to the 31–95 region stimulated IgG production and four peptides (31–45, 46–65, 76–95, and 151–170) stimulated IgM production. These results suggest that various parts of caseins are related to the proliferation stimulating and IPSF activities.

Keywords

Human Lung Adenocarcinoma Immunoglobulin Production HB4C5 Cell Proliferation Stimulate Human Lymphocyte Culture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media Dordrecht 1991

Authors and Affiliations

  • K. Yamada
    • 1
  • H. Nakajima
    • 1
  • L. Ikeda
    • 1
  • S. Shirahata
    • 2
  • A. Enomoto
    • 3
  • S. Kaminogawa
    • 3
  • H. Murakami
    • 2
  1. 1.Department of Food Science and TechnologyKyushu University 46-09Higashi-ku, FukuokaJapan
  2. 2.Graduate School of Genetic Resources Technology, Faculty of AgricultureKyushu University 46-09Higashi-ku, FukuokaJapan
  3. 3.Department of Agricultural ChemistryThe University of TokyoTokyoJapan

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