Effects of Shear Stress on the Growth of Hybridoma Cells Cultivated in Serum-Free Medium Coupled with Ammonia Removing System
Serum-free perfusion cultures of mouse-mouse hybridoma TO-405 producing HBs monoclonal antibody were done in spinner flasks coupled with an ammonia-removing system. Ammonia in the culture broth was effectively maintained below inhibitory concentrations for cell growth. Maximum viable cell density levels similar to that in serum supplemented cultures as well a as higher of percentage viability were obtained using this system. Variation of the cross-flow rate through the ceramic filter between 0.29 m/s to 0.59 m/s did not cause immediate cell damage, but the shear stress generated in the system repressed the growth of the cells at the higher flow rate.
KeywordsCulture Broth Ammonia Removal Perfusion Culture Spinner Flask Animal Cell Culture
Unable to display preview. Download preview PDF.
- 1.Matsumura, M., Misato, T., Kataoka, H., Tanaka, T. and Mayumi, M. (1989) ‘Production of HBs monoclonal antibody by perfusion culture in membrane reactor’, in H. Murakami (editor), Trends in Animal Cell Culture Technology, Kodansha Ltd., Tokyo, pp. 145–148.Google Scholar
- 2.Motoki, M., Matsumura, M. and Kataoka, H. (1988) ‘Removal of ammonia from animal cell culture broth using zeolite’, Abstract of the Annual Meeting of Chemical Engineers, Sendai, pp. 4.Google Scholar