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Use of histochemical techniques to quantify mass /activity relations of mycorrhizal roots and soil

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The Rhizosphere and Plant Growth

Part of the book series: Beltsville Symposia in Agricultural Research ((BSAR,volume 14))

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Abstract

In mycorrhizal research, morphology-based methods are used to estimate the proportion of ectomycorrhizae on tree seedlings. Extramatrical mycelial mass and activity of the mycorrhiza-soil system are not commonly quantified. We used the quantity of ergosterol and the activity of ornithine decarboxylase (ODC) in rhizosphere soil, non-rhizosphere soil and roots of Pinus contorta seedlings ectomycorrhizal with Hebeloma crustuliniforme as analogues of mycelial mass and biotic activity, respectively. The three components were sampled at 3 week intervals for 12 weeks to describe ontogeny of mycorrhizae.

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© 1991 Springer Science+Business Media Dordrecht

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Johnson, B.N., McGill, W.B. (1991). Use of histochemical techniques to quantify mass /activity relations of mycorrhizal roots and soil. In: Keister, D.L., Cregan, P.B. (eds) The Rhizosphere and Plant Growth. Beltsville Symposia in Agricultural Research, vol 14. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3336-4_83

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  • DOI: https://doi.org/10.1007/978-94-011-3336-4_83

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-5473-7

  • Online ISBN: 978-94-011-3336-4

  • eBook Packages: Springer Book Archive

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