• Christos Paraskeva
  • Angela Hague
Part of the Developments in Oncology book series (DION, volume 64)


Cancer development in the colorectum, which is a major site for human malignant disease, is an excellent example of the multistage nature of cancer. Most colorectal cancers develop from adenomas (premalignant tumours sometimes referred to as polyps) in what is called the adenoma carcinoma sequence (Muto et al., 1975). The adenomas are derived from the normal colonic epithelium lining the intestine. The normal epithelium serves as an interface between the organism and the colonic lumen. There are three differentiated cell types of the colonic epithelium: (1) Mucus-containing goblet cells, (2) Columnar (absorptive) cells, and (3) The enteroendocrine cells. All three cell types probably originate from the so-called stem cells which are thought to be present in the base of the crypts.


Primary Culture Colorectal Adenoma Sodium Butyrate Athymic Nude Mouse Small Clump 
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  1. Augeron, C. and Laboisse, C.L. (1984). Emergence of permanently differentiated cell clones in a human colonic cancer cell line in culture after treatment with sodium butyrate. Cancer Res., 44:3961–3969.PubMedGoogle Scholar
  2. Berry, R.D. and Paraskeva, C. (1988). Expression of carcinoembryonic antigen by adenoma and carcinoma derived cell lines: possible marker of tumour progression and modulation of expression by sodium butyrate. Carcinogenesis (Lond.) 9:447–450.CrossRefGoogle Scholar
  3. Brattain, M.G., Brattain, D.E., Sarrif, A.M., McRae, L.J., Fine, W.D. and Hawkins, J.G. (1982). Enhancement of growth of human colon tumour cell lines by feeder layers of murine fibroblasts. J. Nat. Cancer Inst 69: 767–771.PubMedGoogle Scholar
  4. Brattain, M.G., Marks, M.E., McCombs, J., Finely, W. and Brattain, D.E. (1983). Characterization of human colon carcinoma cell lines isolated from a single primary tumour. Br. J. Cancer 47:373–381.PubMedCrossRefGoogle Scholar
  5. Corfield, AP, Clamp, JR, Casey, AD and Paraskeva, C. (1990). Characterization of a sialic rich mucus glycoptrotein secreted by a premalignant human colorectal adenoma cell line. Int. J. Cancer, 46: 1059–106PubMedCrossRefGoogle Scholar
  6. Fogh, J., Fogh, J.M. and Orfeo, T. (1977). One hundred and twenty seven cultured human tumour cell lines producing tumours in nude mice. J. Nat. Cancer. Inst. 59:221–225.PubMedGoogle Scholar
  7. Friedman, E.A., Higgins, P.J., Lipkin, M., Shinya, H. and Gelb, A.M. (1981). Tissue culture of human epithelial cells from benign colonic tumours. In vitro 17:632–644.PubMedCrossRefGoogle Scholar
  8. Kim, Y.S., Tsao, D., Siddiqui, B., Whitehead, J.S., Arnstein, P., Bennet, J. and Hicks, J. (1980). Effects of sodium butyrate and dimethyl sulfoxide on biochemical properties of human colon cancer cells. Cancer (Phila) 45:1185–1192.CrossRefGoogle Scholar
  9. Kirkland, S.C. (1985). Dome formation by a Human Colonic Adenocarcinoma cell line (HCA-7). Cancer Res., 45:3790–379PubMedGoogle Scholar
  10. Kirkland, S.C. and Bailey, I.G. (1986). Establishment and characterization of six human colorectal adenocarcinoma cell lines. Br. J. Cancer 53: 779–785.PubMedCrossRefGoogle Scholar
  11. Laboisse, C.L. (1989). Differentiation of colon cells in culture. In Augenlicht, L.H. (ed): ‘Cell and Molecular Biology of Colon Cancer’ p27–43, CRC Press, Inc. Boca Raton, Florida.Google Scholar
  12. Lane, E.B. (1982). Monoclonal antibodies provide specific intramolecular markers for the study of epithelial tonofilament organisation. J. Cell. Biol. 92:665–673.PubMedCrossRefGoogle Scholar
  13. Leibovitz, A., Stinson, J.C., McComb, W.B., McCoy, C.E., Mazur, K.C. and Mabry, N.D. (1976) Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36:3562–3569.Google Scholar
  14. Maciewicz, R.A., Wardale, R.J., Etherington, D.J. and Paraskeva, C. (1989). Immunodetection of cathepsins B and L present in and secreted from human pre-malignant and malignant colorectal tumour cell lines. Int. J. Cancer 43:478–486.PubMedCrossRefGoogle Scholar
  15. Muto, T., Bussey, H.J.R. and Morson, B.C. (1975). The evolution of cancer of the colon and rectum. Cancer 36: 2251–2270.PubMedCrossRefGoogle Scholar
  16. Paraskeva, C., Buckle, B.G., Sheer, D. and Wigley, C.B. (1984). The isolation and characterization of colorectal epithelial cell lines at different stages in malignant transformation from familial polyposis coli patients. Int. J. Cancer 34:49–56.PubMedCrossRefGoogle Scholar
  17. Paraskeva, C., Buckle, B.G. and Thorpe, P.E. (1985). Selective killing of contaminating human fibroblasts in epithelial cultures derived from colorectal tumours using an anti Thy-1 antibody-ricin conjugate. Br.J.Cancer 51:131–134.PubMedCrossRefGoogle Scholar
  18. Paraskeva, C., Finerty S. and Powell, S.C. (1988). Immortalization of a human colorectal adenoma cell line by continous in vitro passage: possible involvement of chromosome 1 in tumour progression. Int. J. Cancer 41: 908–912.PubMedCrossRefGoogle Scholar
  19. Paraskeva, C., Finerty, S., Mountford, R.A. and Powell, S.C. (1989a). Specific cytogenetic abnormalities in two new human colorectal adenoma-derived epithelial cell lines. Cancer Res 49:1282–1286.PubMedGoogle Scholar
  20. Paraskeva, C., Harvey, A., Finerty, S. and Powell, S.C. (1989b). Possible involvement of chromosome 1 in in vitro immortalization: Evidence from progression of a human adenoma-derived cell line in vitro. Int. J. Cancer 43:743–746.PubMedCrossRefGoogle Scholar
  21. Pinto, M., Appay, M.D., Simon-Assman, P., Chevalier, G., Dracopoli, N., Fogh, J. and Zweibaum A. (1982). Enterocytic differentiation of cultured human colon cancer cells by replacement of glucose by galactose in the medium. Biol. Cell., 44: 193–19Google Scholar
  22. Rheinwald, J.G., and Beckett, M.A. (1981). Tumorigenic keratinocyte lines requiring anchorage and fibroblast support cultured from human squamous cell carcinomas. Cancer Res., 41: 1657–166PubMedGoogle Scholar
  23. Richman, P.I. and Bodmer, W.F. (1987). Monoclonal antibodies to human colorectal epithelium: Markers for Differentiation and Tumour characterization. Int. J. Cancer 39:317–328.PubMedCrossRefGoogle Scholar
  24. Robine, S., Huet, C., Moll, R., Sahuquillo-Merino, C., Coudrier, E., Zweibaum, A. and Louvard, D. (1985). Can Villin be used to identify malignant and undifferentiated normal digestive epithelial cells. Proc. Natl. Acad. Sci. (USA) 82: 8488–8492.CrossRefGoogle Scholar
  25. Tsao, D., Morita, A., Bella, A., Jr., Luu, P. and Kim, Y.S. (1982). Differential effects of sodium butyrate, dimethyl sulfoxide, and retinoic acid on membrane-associated antigen, enzymes, and glycoproteins of human rectal adenocarcinoma cells. Cancer Res. 42:1052–1058.PubMedGoogle Scholar
  26. Willson, J.K.V., Bittner, G.N., Oberley, T.D., Meisner, L.F. and Weese, J.L. (1987) Cell culture of human colon adenomas and carcinomas. Cancer Res. 47:2704–2713.PubMedGoogle Scholar

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© Springer Science+Business Media Dordrecht 1991

Authors and Affiliations

  • Christos Paraskeva
  • Angela Hague

There are no affiliations available

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