• Maureen E. Harper
Part of the Developments in Oncology book series (DION, volume 64)


Although methods for culture of primary prostatic cancer have been reported (Brehmer et al., 1972; Chaproniere and McKeehan, 1986; Malinin et al., 1980; Mistry et al., 1983; Peehl et al., 1986) many have not proved reproducible. Over a period of several years we have investigated a whole spectrum of methods for the primary culture of prostatic tumours both benign and malignant. Considerable success has been achieved with explant culture of benign tumours, over 95% of the specimens giving rise to epithelial monolayers (Webber and Stonington, 1975; Syms et al., 1981); prostatic cancer specimens however have not yielded such good results. Many factors contribute to the poor growth observed, some may be avoided or at least their effects reduced and other factors can be circumvented by the use of particular techniques.


Human Prostate Prostatic Carcinoma Soft Agar Prostatic Carcinoma Cell Anchorage Independent Growth 
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  1. Bregman, U.R. and Bregman, E.T. (1961). Tissue culture of benign and malignant human genitourinary tumours. J. Urol. 86, 642–649.PubMedGoogle Scholar
  2. Brehmer, B., Marquardt, H. and Madsen, P.O. (1972). Growth and hormonal response of cells derived from carcinoma and hyperplasia of the prostate in monolayer cell culture. A possible in vitro model for clinical chemotherapy. J. Urol. 108, 890–896.PubMedGoogle Scholar
  3. Chaproniere, D.M. and McKeehan, W.L. (1986). Serial culture of single adult human prostatic epithelial cells in serum free medium containing low calcium and a new growth factor from bovine brain. Cancer Res. 46, 819–824.PubMedGoogle Scholar
  4. Chaproniere-Rickenberg, D.M. and Webber, M.M. (1982). A chemically defined medium for the growth of adult human prostatic epithelium. In: Growth of Cells in Hormonally Defined Media. 9B, 1109–1115. Eds. Sato, Pardee, Sirbashu. Cold Spring Harbour Laboratory, New York.Google Scholar
  5. Cillo, C., Schreyer, M., Odartchenko, N. and Carrel, S. (1984). Histological analysis of human tumour cell colonies grown in methylcellulose cultures. Br. J. Cancer 49, 653–657.PubMedGoogle Scholar
  6. Clarke, S.M. and Merchant, D.J. (1980). Primary cultures of human prostatic epithelial cells from transurethral resection specimens. Prostate 1, 87–94.PubMedGoogle Scholar
  7. Courtenay, V.D., Selby, P.J., Smith, Mills, J. and Peckham, J. (1978). Growth of human tumour cell colonies from biopsies using two soft agar techniques. Br. J. Cancer 38, 77–81.PubMedGoogle Scholar
  8. Fraley, E.E., Echer, S. and Vincent, M.M. (1970). Spontaneous in vitro neoplastic transformation of adult human prostatic epithelium. Science 170, 540–542.PubMedGoogle Scholar
  9. Franks, L.M. (1980). Primary cultures of human prostate. Methods Cell Biol. 21B, 153–169.PubMedGoogle Scholar
  10. Franks, L.M., Riddle, P.N., Carbonell, A.W. and Gey, G.O. (1970). A comparative study of the ultrastructure and lack of growth capacity of adult human prostate epithelium mechanically separated from its stroma. J. Path. 100, 113–119.PubMedGoogle Scholar
  11. Grossman, H.B., Wedemeyer, G., Ren, L. and Carey, T.E. (1984). UM-SCP-1, a new human cell line derived from prostatic squamous cell carcinoma. Cancer Res. 44, 4111–4117.PubMedGoogle Scholar
  12. Heatfield, B.M. (1987). In vitro models: organ explant culture of normal and neoplastic human prostate. Prog. Clin. Biol. Res. 239, 391–471.PubMedGoogle Scholar
  13. Heatfield, B.M., Sanefuji, H. and Trump, B.F. (1980). Long term explant culture of normal human prostate. Methods Cell Biol. 21B, 191–225.Google Scholar
  14. Horoszewicz, J.S., Leong, S.S., Kawinski, E., Karr, J.P., Rosenthal, H., Ming Chu, T., Mirand, E.A. and Murphy, G.P. (1983). LNCaP Model of human prostatic carcinoma. Cancer Res. 43, 1809–1819.PubMedGoogle Scholar
  15. Kadohama, N., Kirdani, R.Y., Murphy, G.P. and Sandberg, A.A. (1977). 5α-Reductase as a target enzyme for anti-prostatic drugs in organ culture. Oncology 34, 123–128.PubMedGoogle Scholar
  16. Kaighn, M.E. and Babcock, M.S. (1975). Monolayer cultures of human prostatic cells. Cancer Chemother. Rep. 59, 59–63.PubMedGoogle Scholar
  17. Kaighn, M.E., Lechner, J.F., Babcock, M.S., Marnell, M., Ohnuki, Y. and Narayan, S.K. (1980). The Pasadena cell lines. Prog. Clin. Biol. Res. 37, 85–109.PubMedGoogle Scholar
  18. Kaighn, M.E., Shankar Narayan, K., Ohnuki, Y., Lechner, J.F. and Jones, L.W. (1979). Establishment and characterization of a human prostatic carcinoma cell line (PC-3). Invest. Biol. 17, 16–23.Google Scholar
  19. Kreisberg, J.I., Brattain, M.G. and Pretlow II, T.G. (1977). Studies on human hyperplastic prostate maintained in organ culture. Invest. Urol. 15, 252–255.PubMedGoogle Scholar
  20. Lechner, J.F. (1987). Replicative cultures of human prostatic epithelial cells. Prog. Clin. Biol. Res. 239, 497–509.PubMedGoogle Scholar
  21. Lechner, J.F., Babcock, M.S., Marnell, M., Shankar Narayan, K. and Kaighn, M.E. Normal human prostate epithelial cell cultures. In ‘Methods in Cell Biology’ 21B, 195–225 (1980) London: Academic Press.Google Scholar
  22. Lechner, J.F., Shankar Narayan, K., Ohnuki, Y., Babcock, M.S., Jones, L.W. and Kaighn, M.E. (1978). Replicative epithelial cell cultures from normal human prostate gland. Brief communication. J. Natl. Cancer Inst. 60,797–799.PubMedGoogle Scholar
  23. Leibovitz, A. (1986). Development of tumour cell lines. Cancer Genet. Cytogenet. 19, 11–19.PubMedGoogle Scholar
  24. Lubaroff, D.M. (1978). HPC-36: An epithelial tissue culture line derived from human prostate adenocarcinoma. Natl. Cancer Inst. Monogr. 49, 35–39.PubMedGoogle Scholar
  25. Malinin, T.I., Claflin, A.J., Block, L.N. and Brown, A.L. (1980). Establishment of primary cell cultures from normal and neoplastic human prostate gland tissue. Prog. Clin. Biol. Res. 37, 161–180.PubMedGoogle Scholar
  26. McMahon, M.J. and Thomas, G.H. (1973). Morphological changes of benign prostatic hyperplasia in culture. Br. J. Cancer 27, 323–335.PubMedGoogle Scholar
  27. Merchant, D.J. (1987). Primary culture as an in vitro model. Prog. Clin. Biol. Res. 239, 473–495.PubMedGoogle Scholar
  28. Merchant, D.J., Clarke, S.M., Ives, K. and Harris, S. (1983). Primary explant culture: An in vitro model of the human prostate. Prostate 4, 523–542.PubMedGoogle Scholar
  29. Mistry, D., Weaver, J.P. and Riches, A. (1983). Organ culture studies of human prostatic adenocarcinomas. Prostate 4, 307–314.PubMedGoogle Scholar
  30. Peehl, D.M. and Stamey, T.A. (1984). Serial propagation of adult human prostatic epithelial cells with cholera toxin. In Vitro 20, 981–986.PubMedGoogle Scholar
  31. Peehl, D.M. and Stamey, T.A. (1986). Growth responses of normal, benign hyperplastic and malignant human prostatic cells in vitro to cholera toxin, pituitary extract and hydrocortisone. Prostate 8, 51–61.PubMedGoogle Scholar
  32. Peehl, D.M., Wong, S.T. and Stamey, T.A. (1988). Clonal growth characteristics of adult human prostatic epithelium cells. In Vitro Cell Dev. Biol. 24, 530–536.PubMedGoogle Scholar
  33. Rohl, L. (1959). Prostatic hyperplasia and carcinoma studied with tissue culture technique. Acta Scand. Suppl. 240, 7–8.Google Scholar
  34. Rose, N.R., Choe, B.K. and Pontes, J.E. (1975). Cultivation of epithelial cells from the prostate. Cancer Chemother. Rep. 59, 147–149.PubMedGoogle Scholar
  35. Schroder, F.H. and Mackensen, S.J. (1974). Human prostatic adenoma and carcinoma in cell culture. The effects of androgen-free culture medium. Invest. Urol. 12, 176–181.Google Scholar
  36. Schroeder, F.H., Sato, G. and Gittes, R.F. (1971). Human prostatic adenocarcinoma: Growth in monolayer tissue culture. J. Urol. 106, 734–739.PubMedGoogle Scholar
  37. Stone, R.K., Mickey, D.D., Wunderli, H., Mickey, G.H. and Paulson, D.F. (1978). Isolation of a human prostatic carcinoma cell line (DU 145). Int. J. Cancer 21, 274–281.PubMedGoogle Scholar
  38. Stone, K.R., Paulson, D.F., Bonar, R.A. and Reich, G.F. III (1975). In vitro culture of epithelial cells derived from urogenital tissue. Urol. Res. 2, 149–153.PubMedGoogle Scholar
  39. Stonington, O.G. and Hemmingsen, H. (1971). Culture of cells as a monolayer derived from the epithelium of the human prostate: A new cell growth technique. J. Urol. 106, 393–400.PubMedGoogle Scholar
  40. Stonington, O.G., Szwec, N. and Webber, M. (1975). Isolation and identification of the human malignant prostatic epithelial cell in pure monolayer culture. J. Urol. 114, 903–908.PubMedGoogle Scholar
  41. Syms, A.J., Harper, M.E., Battersby, S. and Griffiths, K. (1981). Proliferation of human prostatic epithelial cells in culture. Aspects of identification. J. Urol. 121, 561–567.Google Scholar
  42. Webber, M.M. (1974). Effects of serum on the growth of prostatic cells in vitro. J. Urol. 112, 798–801.PubMedGoogle Scholar
  43. Webber, M.M. (1980). Growth and maintenance of normal prostatic epithelium In Vitro — A human cell model. Prog. Clin. Biol. Res. 37, 181–216.PubMedGoogle Scholar
  44. Webber, M.M. (1979). Normal and benign human prostatic epithelium in culture. In Vitro 15, 967–982.PubMedGoogle Scholar
  45. Webber, M.M., Stonington, O.E. and Poche, P.A. (1974). Epithelial outgrowth from suspension cultures of human prostatic tissue. In Vitro 10, 196–205.PubMedGoogle Scholar
  46. Webber, M.M. and Stonington, O.G. (1975). Stromal hypocellularity and encapsulation in organ culture of human prostate: Application in epithelial cell isolation. J. Urol. 114, 246–248.PubMedGoogle Scholar

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© Springer Science+Business Media Dordrecht 1991

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  • Maureen E. Harper

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