Although methods for culture of primary prostatic cancer have been reported (Brehmer et al., 1972; Chaproniere and McKeehan, 1986; Malinin et al., 1980; Mistry et al., 1983; Peehl et al., 1986) many have not proved reproducible. Over a period of several years we have investigated a whole spectrum of methods for the primary culture of prostatic tumours both benign and malignant. Considerable success has been achieved with explant culture of benign tumours, over 95% of the specimens giving rise to epithelial monolayers (Webber and Stonington, 1975; Syms et al., 1981); prostatic cancer specimens however have not yielded such good results. Many factors contribute to the poor growth observed, some may be avoided or at least their effects reduced and other factors can be circumvented by the use of particular techniques.
KeywordsHuman Prostate Prostatic Carcinoma Soft Agar Prostatic Carcinoma Cell Anchorage Independent Growth
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