Abstract
SAA elevations in the serum lipoproteins of hamsters were induced by injection of lipopolysaccharide intraperitoneally or turpentine subcutaneously. Lipoprotein fractions isolated by sequential density centrifugation of serum samples obtained 20-hours post-stimulus were examined by SDS-PAGE followed by electroimmunoblotting. We observed a marked enhancement of a 12-kDa doublet in the electrophoretic pattern of the acute-phase HDL3. Antibodies to human or monkey AA reacted preferentially with the leading member of the doublet, whereas antibodies to mouse AA reacted preferentially with the trailing member. Antibodies to human and mouse SAA and to duck AA reacted with both members. The different reactions displayed by the members of the hamster SAA doublet against the several antibody preparations suggest that at least two of the three proteins predicted by a study of SAA-gene expression in the hamster may circulate in the HDL.
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© 1991 Springer Science+Business Media Dordrecht
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Eriksen, N., Meek, R.L., Benditt, E.P. (1991). Serum Amyloid A (SAA) Induction in the Serum High Density Lipoproteins of the Syrian Hamster. In: Natvig, J.B., et al. Amyloid and Amyloidosis 1990. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3284-8_25
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DOI: https://doi.org/10.1007/978-94-011-3284-8_25
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