Abstract
In the search for an in vitro model of SAA synthesis we discovered that the human hepatoma cell line HuH-7 responded to recombinant IL-1 by increasing SAA synthesis by up to 100 fold. The dose response curve for IL-1 reached a maximum at about 1 ng/ml and half-maximal stimulation was at 100 pg/ml. The presence of IL-6 further stimulated levels by at least twofold. Leukaemia inhibitory factor (LIF;HSF III) had limited effect and TNF could stimulate SAA but less than IL-6. However the cells express IL-6 receptors since they have the ability to synthesise haptoglobin in response to IL-6 but poorly in response to IL-1. Neutralising monoclonal antibodies against IL-1α and IL-6 selectively neutralised the response due to the respective cytokine.
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© 1991 Springer Science+Business Media Dordrecht
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Raynes, J.G., Eagling, S., McAdam, K.P.W.J. (1991). Regulation of SAA Synthesis by Cytokines in a Human Hepatoma Cell Line.. In: Natvig, J.B., et al. Amyloid and Amyloidosis 1990. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3284-8_13
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DOI: https://doi.org/10.1007/978-94-011-3284-8_13
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