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Neuro-Oncology pp 213-215 | Cite as

8-Chloro-Cyclic Adenosine Monophosphate is a Potent Inhibitor of Human Glioma Cell Proliferation In Vitro

  • C. H. Langeveld
  • M. P. Van Waas
  • J. J. Heimans
  • J. C. Stoof
Part of the Developments in Oncology book series (DION, volume 66)

Abstract

Cyclic adenosine 3′,5′-monophosphate (cAMP) is a second messenger playing an important role in cell growth and differentiation [1]. Since cAMP itself hardly enters the cell, the role of cAMP has been demonstrated by the use of cAMP analogs such as dibutyryl cAMP (dbcAMP). DbcAMP produces significant growth delay in multicellular spheroids derived from human brain tumours [2] and has also been found to induce morphological differentiation in human glioma cells [3]. However, usually the concentration of dbcAMP in these studies is very high (1 mM). Recently, cAMP analogs have been identified that selectively bind to one of the two known cAMP binding sites on the regulatory units of the cAMP dependent protein kinase (PKA). These site-selective analogs induce growth inhibition and differentiation in several human cancer cell lines at much lower concentrations than dbcAMP [1]. In the present study we compared the effects of the site-selective analog 8-chloro-cAMP (8ClcAMP) on human glioma cells to the effects of more commonly used cAMP analogs, such as dbcAMP.

Keywords

Glioma Cell Human Cancer Cell Line Human Glioma Cell cAMP Dependent Protein Kinase Human Brain Tumour 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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    Cho-Chung, Y.S., Clair, T., Tagliaferri, P. et al. (1989) ‘Site-selective cyclic AMP analogs as new biological tools in growth control, differentiation, and proto-oncogene regulation’, Cancer Invest 7, 161–177.PubMedCrossRefGoogle Scholar
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    Oktar, N., Darling, J.L. and Thomas, D.G.T. (1987) ‘An experimental trial of cyclic nucleotides on multicellular spheroids derived from human brain tumours’, J Neuro-Onc 5, 83–89.CrossRefGoogle Scholar
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    Edström, A., Kanje, M. and Walum, E. (1974) ‘Effects ofdibutyryl cyclic AMP and prostaglandin El on cultured human glioma cells’, Exp Cell Res 85, 217–223.PubMedCrossRefGoogle Scholar
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    Alley, M.C., Scudiero, D.A., Monks, A. et al. (1988) ‘Feasability of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay’, Cancer Res 48, 589–601.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 1991

Authors and Affiliations

  • C. H. Langeveld
    • 1
  • M. P. Van Waas
    • 1
  • J. J. Heimans
    • 1
  • J. C. Stoof
    • 1
  1. 1.Department of Neurology, Medical FacultyFree UniversityAmsterdamThe Netherlands

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