Abstract
Glucocorticoid hormone high affinity binding activity has been demonstrated in human brain tumor cell extracts by radiolabelled ligand assay. To determine the molecular species responsible for this activity and their cellular distribution, we studied by in situ hybridization, with a human glucocorticoid receptor (hGR) cDNA digoxigenin probe, the expression of hGR mRNA in 10 brain tumors (3 astrocytomas, 1 glioblastoma, 1 neurinoma, 3 meningiomas, 1 choroid plexus papilloma, 1 ependymoma). All tumors, with the exception of the glioblastoma and one fibroblastic meningiomas, contained hGR positive neoplastic cells. The intensity of positivity varied between different tumors and within the same tumor. These results indicate, for the first time, the presence of true hGR receptor mRNA in human brain tumor cells.
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© 1991 Springer Science+Business Media Dordrecht
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Magrassi, L., Silini, E., Brambilla, G.L., Assietti, R., Milanesi, G., Butti, G. (1991). Cellular Localization of Glucocorticoid Receptor mRNAs in Human CNS Tumors by In Situ Hybridization. In: Paoletti, P., Takakura, K., Walker, M.D., Butti, G., Pezzotta, S. (eds) Neuro-Oncology. Developments in Oncology, vol 66. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3152-0_11
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DOI: https://doi.org/10.1007/978-94-011-3152-0_11
Publisher Name: Springer, Dordrecht
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