Skip to main content
SpringerLink
Log in

Fibrinopeptide A (FPA)

  • Chapter
ECAT Assay Procedures A Manual of Laboratory Techniques

  • 96 Accesses

Abstract

Fibrinopeptide A (FPA) is of particular interest, because it is released whenever thrombin converts fibrinogen to fibrin. Human FPA is a 16 amino acid peptide with a molecular weight of 1540, and it is cleaved from the N-terminal part of the Aα-chain of fibrinogen. Since the fibrinogen molecule has a dimer structure, two identical FPA molecules are cleaved from each fibrinogen molecule1.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Marder VJ, Francis CW, Doolittle RF Fibrinogen, structure and physiology. In: Colman RW, Hirsh J, Marder VJ, Salzman EW (eds), Haemostasis and thrombosis. Philadelphia: Lippincott, 1982; 145–63.

    Google Scholar 

  2. Nossel HL, Younger LR, Wilner GD, Procupez D, Canfield RE, Butler VP Radioimmunoassay of human fibrinopeptide A. Proc Natl Acad Sci USA 1971; 68: 2350–3.

    Article  PubMed  CAS  Google Scholar 

  3. Nossel HL, Yudelman I, Canfield RE, Butler VP, Spanondis K, Wilner GD, Qureshi GD Measurement of fibrinopeptide A in human blood. J Clin Invest 1974; 54: 43–53.

    Article  PubMed  CAS  Google Scholar 

  4. Bauer KA, Rosenberg RD The pathophysiology of the prethrombotic state in humans: Insights gained from studies using markers of hemostatic system activation. Blood 1987; 70: 343–50.

    PubMed  CAS  Google Scholar 

  5. Hirsh J Blood tests for the diagnosis of venous and arterial thrombosis. Blood 1981; 57: 1–8.

    PubMed  CAS  Google Scholar 

  6. Joist JH Fibrinopeptide A in the diagnosis and treatment of deep venous thrombosis and pulmonary embolism. Clin Lab Med 1984; 4: 363–80.

    PubMed  CAS  Google Scholar 

  7. Douglas IT, Blarney SL, Lowe GDO, Carter DC, Forbes CD Plasma beta-thromboglobulin, fibrinopeptide A and Bß 15–42 antigen in relation to postoperative DVT, malignancy and stanozolol treatment. Thromb Haemostas 1985; 53: 235–8.

    CAS  Google Scholar 

  8. Leeksma OC, Meijer-Huizinga F, Stoepman-van-Dalen EA, van Ginkel CJW, van Aken WG, van Mourik JA Fibrinopeptide A and the phosphate content of fibrinogen in venous thromboembolism and disseminated intravascular coagulation. Blood 1986; 67: 1460–7.

    PubMed  CAS  Google Scholar 

  9. Nossel HL Radioimmunoassay of fibrinopeptides in relation to intravascular coagulation and thrombosis. N Engl J Med 1976; 295: 428–32.

    Article  PubMed  CAS  Google Scholar 

  10. Van Hulsteijn H, Briet E, Koch C, Hermans J, Bertina R Diagnostic value of fibrinopeptide A and beta-thromboglobulin in acute deep venous thrombosis and pulmonary embolism. Acta Med Scand 1982; 211: 323–30.

    Article  PubMed  Google Scholar 

  11. Yudelman IM, Nossel HL, Kaplan KL, Hirsh J Plasma fibrinopeptide A levels in symptomatic venous thromboembolism. Blood 1978; 51: 1189–95.

    PubMed  CAS  Google Scholar 

  12. Cronlund M, Hardin J, Burton J, Lee L, Haber E, Bloch KJ Fibrinopeptide A in plasma of normal subjects and patients with disseminated intravascular coagulation and systemic lupus erythematosus. J Clin Invest 1976; 58: 142–51.

    Article  PubMed  CAS  Google Scholar 

  13. Mombelli G, Monotti R, Haeberli A, Straub PW Relationship between fibrinopeptide A and fibrinogen/fibrin fragment E in thromboembolism, DIC and various non-thromboembolic diseases. Thromb Haemostas 1987; 58: 758–63.

    CAS  Google Scholar 

  14. Eisenberg PR, Lucore C, Kaufman L, Sobel BE, Jaffe AS, Rich S Fibrinopeptide A levels indicative of pulmonary vascular thrombosis in patients with primary pulmonary hypertension. Circulation 1990; 82: 841–7.

    Article  PubMed  CAS  Google Scholar 

  15. Douglas JT, Lowe GDO, Forbes CD, Prentice CRM Plasma fibrinopeptide A and beta-thromboglobulin in patients with chest pain. Thromb Haemostas 1983; 50: 541–2.

    CAS  Google Scholar 

  16. Eisenberg PR, Sherman LA, Schectman K, Perez J, Sobel BE, Jaffe AS Fibrinopeptide A: a marker of acute coronary thrombosis. Circulation 1985; 71: 912–18.

    Article  PubMed  CAS  Google Scholar 

  17. Gallino A, Haeberli A, Baur HR, Straub PW Fibrin formation and platelet aggregation in patients with severe coronary artery disease: relationship with the degree of myocardial ischemia. Circulation 1985; 72: 27–30.

    Article  PubMed  CAS  Google Scholar 

  18. Gallino A, Haeberli A, Hess T, Mombelli G, Straub PW Fibrin formation and platelet aggregation in patients with acute myocardial infarction: effects of intravenous and subcutaneous low-dose heparin. Am Heart J 1986; 112: 285–90.

    Article  PubMed  CAS  Google Scholar 

  19. Gensini GF, Rostagno C, Abbate R, Favilla S, Mannucci PM, Neri Semeri GG Increased protein C and fibrinopeptide A concentration in patients with angina. Thromb Res 1988; 50: 517–25.

    Article  PubMed  CAS  Google Scholar 

  20. Irie T, Imaaizumi T, Matuguchi T, Koyanagi S, Kanaide H, Takeshita A, Nakamura M Increased fibrinopeptide A during anginal attacks in patients with variant angina. J Am Coll Cardiol 1989; 14: 589–94.

    Article  PubMed  CAS  Google Scholar 

  21. Mombelli G, Im Hof V, Haeberli A, Straub PW Effect of heparin on plasma fibrinopeptide A (fpA) in acute myocardial infarction. Circulation 1984; 69: 684–9.

    Article  PubMed  CAS  Google Scholar 

  22. Neri Semeri G, Gensini GF, Camovali M, Prisco D, Rogasi PG, Casolo GC, Fazi A, Abbate R Association between time of increased fibrinopeptide A levels in plasma and episodes of spontaneous angina: A controlled prospective study. Am Heart J 1987; 113: 672–8.

    Article  Google Scholar 

  23. Nichols AB, Owen J, Kaplan KL, Sciacca RR, Cannon PJ, Nossel HL Fibrinopeptide A, platelet factor 4, and p-thromboglobulin levels in coronary heart disease. Blood 1982; 60: 650–4.

    PubMed  CAS  Google Scholar 

  24. Theroux P, Latour JG, Leger-Gauthier C, De Lara J Fibrinopeptide A and platelet factor levels in unstable angina pectoris. Circulation 1987; 75: 156–62.

    Article  PubMed  CAS  Google Scholar 

  25. Van Hulsteijn H, Kolff J, Briet E, van der Laarse A, Bertina R Fibrinopeptide A and beta-thromboglobulin in patients with angina pectoris and acute myocardial infarction. Am Heart J 1984; 107: 39–45.

    Article  PubMed  Google Scholar 

  26. Landi G, Barbarotto R, Morabito A, D’Angelo A, Mannucci PM Prognostic significance of fibrinopeptide A in survivors of cerebral infarction. Stroke 1990; 21: 424–7.

    Google Scholar 

  27. Conway EM, Bauer KA, Barzegar S, Rosenberg RD. Suppression of hemostatic system activation by oral anticoagulants in the blood of patients with thrombotic diseases. J Clin Invest 1987; 80: 1535–44.

    Article  PubMed  CAS  Google Scholar 

  28. Peuscher FW, van Aken WG, Flier OThN, Stoepman-van Dalen EA, Cremer-Goote TM, van Mourik JA Effect of anticoagulant treatment measured by fibrinopeptide A (fpA) in patients with venous thromboembolism. Thromb Res 1980; 18: 33–43.

    Article  PubMed  CAS  Google Scholar 

  29. Yudelman I, Greenberg J Factors affecting fibrinopeptide A levels in patients with venous thromboembolism during anticoagulant therapy. Blood 1982; 59: 787–92.

    Google Scholar 

  30. Auger MJ, Galloway MJ, Leinster SJ, McVerry BA, Mackie MJ Elevated fibrinopeptide A levels in patients with clinically localised breast carcinoma. Haemostasis 1987; 17: 336–9.

    PubMed  CAS  Google Scholar 

  31. Edwards RL, Klaus M, Matthews E, McCullen C, Bona RD, Rickles FR Heparin abolishes the chemotherapy-induced increase in plasma fibrinopeptide A levels. Am J Med 1990; 89: 25–8.

    Article  PubMed  CAS  Google Scholar 

  32. Gugliotta L, Vigano S, D’Angelo A, Guarini A, Tura S, Mannucci PM High fibrinopeptide A (FPA) levels in acute non-lymphocytic leukemia are reduced by heparin administration. Thromb Haemostas 1984; 52: 301–4.

    CAS  Google Scholar 

  33. Mombelli G, Roux A, Haeberli A, Straub PW Comparison of 125I-fibrinogen kinetics and fibrinopeptide A in patients with disseminated neoplasias. Blood 1982; 60: 381–8.

    PubMed  CAS  Google Scholar 

  34. Myers TJ, Rickles FR, Barb C, Cronlund M Fibrinopeptide A in acute leukemia: Relationship of activation of blood coagulation to disease activity. Blood 1981; 57: 518–25.

    PubMed  CAS  Google Scholar 

  35. Peuscher FW, Cleton FJ, Armstrong L, Stoepman-van-Dalen EA, van Mourik JA, van Aken WG Significance of plasma fibrinopeptide A (fpA) in patients with malignancy. J Lab Clin Med 1980; 96: 5–14.

    PubMed  CAS  Google Scholar 

  36. Douglas JT, Shah M, Lowe GDO, Belah JJF, Forbes CD, Prentice CRM. Plasma fibrinopeptide A and p-thromboglobulin in preeclampsia and pregnancy hypertension. Thromb Haemostas 1982; 47: 54–8.

    CAS  Google Scholar 

  37. Jones RL Fibrinopeptide A in diabetes mellitus. Relation to levels of blood glucose, fibrinogen disappearance and hemodynamic changes. Diabetes 1985; 34: 836–43.

    Article  PubMed  CAS  Google Scholar 

  38. Librenti MC, D’Angelo A, Micossi P, Garimberti B, Mannucci PM, Pozza G. p-thromboglobuIin and fibrinopeptide A in diabetes mellitus as markers of vascular damage. Acta Diabetol Lat 1985; 22: 39–45.

    Article  PubMed  CAS  Google Scholar 

  39. Rosove MH, Frank HJL, Harwig SSL Plasma p-thromboglobulin, platelet factor 4, fibrinopeptide A, and other hemostatic functions during improved, short-term glycemic control in diabetes mellitus. Diabetes Care 1984; 7: 174–9.

    Article  PubMed  CAS  Google Scholar 

  40. Roy MS, Podgar MJ, Rick ME Plasma fibrinopeptide A, p-thromboglobulin, and platelet factor 4 in diabetic retinopathy. Invest Opthalmol Vis Sci 1988; 29: 856–60.

    CAS  Google Scholar 

  41. Coccheri S, Mannucci PM, Palaretti G, Gervasoni W, Poggi M, Vigano S Significance of plasma fibrinopeptide A and high molecular weight fibrinogen in patients with liver cirrhosis. Br J Haematol 1982; 52: 503–9.

    Article  PubMed  CAS  Google Scholar 

  42. Marongiu F, Mameli G, Acca MR, Mulas G, Medda A, Tronci MB, Mamusa AM, Balestrieri A Fibrinopeptide A and BP 15-42 in liver cirrhosis. Haemostasis 1988; 18: 126–28.

    PubMed  CAS  Google Scholar 

  43. Alkjaersig N, Fletcher AP, de Ziegler D, Steingold KA, Meldrum DR, Judd HL Blood coagulation in postmenopausal women given oestrogen treatment: comparison of transdermal and oral administration. J Lab Clin Med 1988; 111: 224–8.

    PubMed  CAS  Google Scholar 

  44. Inauen W, Baumgartner HR, Haeberli A, Straub PW Excessive deposition of fibrin, platelets and platelet thrombi on vascular subendothelium during contraceptive drug treatment. Thromb Haemostas 1987; 57: 306–9.

    CAS  Google Scholar 

  45. Melis GB, Fruzzetti F, Paoletti AM, Carmassi F, Fioretti P Fibrinopeptide A plasma levels during low-estrogen oral contraceptive treatment. Contraception 1984; 30: 575–83.

    Article  PubMed  CAS  Google Scholar 

  46. Melis GB, Fruzzetti F, Ricci C, Carmassi F, Fioretti P Oral contraceptives and venous thromboembolic disease: the effect of the oestrogen dose. Maturitas, Suppl. 1988; 1: 131–9.

    Article  PubMed  Google Scholar 

  47. Hofmann V, Straub PW A radioimmunoassay technique for the rapid measurement of human fibrinopeptide A. Thromb Res 1977; 11: 171–81.

    Article  PubMed  CAS  Google Scholar 

  48. Kockum C, Frebelius S Rapid radioimmunoassay of human fibrinopeptide A — removal of cross-reacting fibrinogen with bentonite. Thromb Res 1980; 19: 589–98.

    Article  PubMed  CAS  Google Scholar 

  49. Soria J, Soria C, Ryckewaert JJ A solid phase immuno enzymological assay for the measurement of human fibrinopeptide A. Thromb Res 1980; 20: 425–35.

    Article  PubMed  CAS  Google Scholar 

  50. Alkjaersig N, Fletcher AP Catabolism and excretion of fibrinopeptide A. Blood 1982; 60: 148–56.

    PubMed  CAS  Google Scholar 

  51. Gallino A, Haeberli A, Straub PW Fibrinopeptide A excretion in urine in patients with atherosclerotic artery disease. Thromb Res 1985; 38: 237–44.

    Article  Google Scholar 

  52. Leeksma OC, Meijer-Huizinga F, Stoepman-van Dalen EA, van Aken WG, van Mourik JA Fibrinopeptide A in urine from patients with venous thromboembolism, disseminated intravascular coagulation and rheumatoid arthritis — Evidence for dephosphorylation and carboxy terminal degradation of the peptide by the kidney. Thromb Haemostas 1985; 54: 792–8.

    CAS  Google Scholar 

  53. Wilensky RL, Zeller JA, Wish M, Tulchinsky M Urinary fibrinopeptide A levels in ischemic heart disease. J Am Coll Cardiol 1989; 14: 597–603.

    Article  PubMed  CAS  Google Scholar 

  54. Kudryk B, Gidlund M, Rohoza A, Ahadi M, Coiffe D, Weitz JI Use of a synthetic homologue of human fibrinopeptide A for production of a monoclonal antibody specific for the free peptide. Blood 1989; 74: 1036–44.

    PubMed  CAS  Google Scholar 

Download references

Authors
  1. A. Haeberli
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Clinical Chemistry, Ribe County Hospital in Esbjerg, Esbjerg, Denmark

    Jørgen Jespersen MD DSc

  2. Haemostasis and Thrombosis Research Unit, University Hospital Leiden, Leiden, The Netherlands

    Rogier M. Bertina PhD

  3. Gaubius Laboratory, IVVO-TNO, Leiden, The Netherlands

    F. Haverkate MD

Rights and permissions

Reprints and permissions

Copyright information

© 1992 Springer Science+Business Media Dordrecht

About this chapter

Cite this chapter

Haeberli, A. (1992). Fibrinopeptide A (FPA). In: Jespersen, J., Bertina, R.M., Haverkate, F. (eds) ECAT Assay Procedures A Manual of Laboratory Techniques. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2992-3_13

Download citation

  • DOI: https://doi.org/10.1007/978-94-011-2992-3_13

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-5330-3

  • Online ISBN: 978-94-011-2992-3

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation