In situ hybridization for molecular cytogenetics

  • A. K. Raap
  • C. J. Cornelisse


After the biochemical description of the principles of nucleic acid hybridization in the sixties, three groups reported independently in 1969–1970 its application for the microscopic detection of specific nucleic acid sequences (Pardue and Gall, 1969; John et al., 1969; Buongiorno-Nordelli and Amaldi, 1970). In contrast to the first microscopic work done with fluorescent antibody probes, the labels were radioisotopes, the detection of which was accomplished by micro-autoradiography. The disadvantages inherent to the use of radioisotopes (poor topological resolution, environmental and health hazards, complex multiple sequence detection) prompted several groups to develop non-isotopic nucleic acid detection techniques.


Interphase Nucleus Restriction Fragment Length Polymorphism Molecular Cytogenetic Numerical Chromosome Aberration Interphase Cytogenetic 
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Copyright information

© Springer Science+Business Media Dordrecht 1992

Authors and Affiliations

  • A. K. Raap
    • 1
  • C. J. Cornelisse
    • 2
  1. 1.Department of Cytochemistry and CytometryMedical Faculty, Leiden UniversityLeidenThe Netherlands
  2. 2.Department of PathologyMedical Faculty, Leiden UniversityLeidenThe Netherlands

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