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The use of in situ hybridization in studies of viral disease

  • Adrienne L. Morey
  • Kenneth A. Fleming
Chapter
  • 35 Downloads

Abstract

In situ hybridization has a number of features which make it particularly suitable for use in studies of viral disease. Not only can it confirm the presence of specific viral DNA or RNA sequences in a range of histological preparations, but by demonstrating the precise tissue, cellular and subcellular location of the virus it can correlate the presence of a virus with its pathological effects and provide insight into the mechanisms involved in virus-host cell interactions. Such information is necessarily lost when the more conventional technique of dot (filter) hybridization of extracted nucleic acid, or the more recently described polymerase chain reaction (PCR) are used to detect viral sequences. As well as localizing viral genomes in the episomal or integrated state, in situ detection of viral mRNA is possible. This can provide valuable information about the level of viral gene expression and sites of viral protein synthesis. In addition, in situ hybridization can be combined sequentially with immunohistological labelling of either cellular antigens (to identify unequivocally the cell types infected) or viral antigens (to determine whether viral nucleic acid is being translated into protein products), thus increasing the amount of information available from a given sample of tissue.

Keywords

Human Papilloma Virus Viral Genome Hybridization Study Hepatitis Delta Virus Viral Nucleic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Aksamit AJ, Mourrain P, Sever JL and Major EO (1985) Progressive multifocal leucoence-phalopathy: investigation of three cases using in situ hybridization with JC virus biotinylated probe. Ann. Neurol. 18: 490–496.PubMedGoogle Scholar
  2. Alex andersen S, Bloom ME, Wolfinbarger J and Race RE (1987) In situ molecular hybridization for detection of Aleutian mink disease parvovirus DNA by using str and-specific probes: identification of target cells for viral replication in cell cultures and in mink kits with virus-induced interstitial pneumonia. J Virol. 61: 2407–2419.PubMedGoogle Scholar
  3. Ballinger ME, Rice CM and Miller BR (1988) Detection of yellow fever virus nucleic acid in infected mosquitoes by RNA:RNA in situ hybridization. Mol. Cell. Probes 2: 331–338.PubMedGoogle Scholar
  4. Bamborschke S, Porr A, Huber M and Heiss WD (1990) Demonstration of herpes simplex virus DNA in CSF cells by in situ hybridization for early diagnosis of herpes encephalitis. J. Neurol. 237: 73–76.PubMedGoogle Scholar
  5. Baskar JF, Stanat SC and Huang S (1986) Murine cytomegalovirus infection of mouse testes. J.Virol. 57: 1149–1154.PubMedGoogle Scholar
  6. Beckmann AM, Myerson D, Daling JR, Kiviat NB, Fenoglio CM and McDougall JK (1985) Detection and localization of human papillomavirus DNA in human genital condylomas by in situ hybridization with biotinylated probes. J. Med. Virol. 16: 265–273. Beckmann AM, Darling JR, Sherman KJ, Maden C, Miller BA, Coates RJ, Kiviat NBPubMedGoogle Scholar
  7. Myerson D, Weiss NS and Hislop TG (1989) Human papilloma virus infection and anal cancer. Int. J. Cancer 43: 1042–1049.PubMedGoogle Scholar
  8. Benditt EP, Barrett T and McDougall JK (1983) Viruses in the etiology of atherosclerosis. Proc. Natl. Acad. Sci. USA 80: 6386–6389.PubMedGoogle Scholar
  9. Blum HE, Haase AT and Vyas GN (1984) Molecular pathogenesis of hepatitis B infection: simultaneous detection of viral DNA and antigens in paraffin-embedded liver sections. Lancet 2: 771–775.PubMedGoogle Scholar
  10. Bornkamm GW, Desgranges C and Gissman L (1983) Nucleic acid hybidization for the detection of viral genomes. Curr. Top. Microbiol. Immunol. 104: 287–298.PubMedGoogle Scholar
  11. Boyle AL, Ballard SG and Ward DC (1990) Differential distribution of long and short interspersed element sequences in the mouse genome: Chromosome karyotyping by fluorescence in situ hybridization. Proc. Natl. Acad. Sci. USA 87: 7757–7761.PubMedGoogle Scholar
  12. Brahic M, Stowring L, Ventura P and Haase AT (1981) Gene expression in visna virus infection in sheep. Nature 292: 240–242.PubMedGoogle Scholar
  13. Brahic M, Haase AT and Cash E (1984) Simultaneous in situ detection of viral RNA and antigens. Proc. Natl. Acad. Sci. USA 81: 5445–5448.PubMedGoogle Scholar
  14. Brahic M and Haase AT (1989) Double-label techniques of in situ hybridization and immunocyto-chemistry. Curr. Top. Microbiol. Immunol. 143: 9–20.PubMedGoogle Scholar
  15. Brambilla C, Tackney C, Hirschman SZ, Colombo M, Dioguardi ML, Donato MF and Paronetto F (1986) Varying nuclear staining intensity of hepatitis B virus DNA in human hepatocellular carcinoma. Lab. Invest. 55: 475–481.PubMedGoogle Scholar
  16. Brigati DJ, Myerson D, Leary JJ, Spalholz B, Travis SZ, Fong CKY, Hsiung GD and Ward DC (1983) Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labelled hybidization probes. Virology 126: 32–50.PubMedGoogle Scholar
  17. Burns J, Graham AK, Frank C, Fleming KA, Evans MF and McGee J (1987) Detection of low copy human papilloma virus DNA and mRNA in routine paraffin sections of cervix by non-isotopic in situ hybridisation. J. Clin. Pathol. 40: 858–864.PubMedGoogle Scholar
  18. Burns J, Graham AK and McGee J (1988) Non-isotopic detection of in situ nucleic acid in cervix: an updated protocol. J. Clin. Pathol. 41: 897–899.PubMedGoogle Scholar
  19. Coghlan JP, Aldred P, Haralambidis J, Niall HD, Penschow JD and Tregear GW (1985) Hybridization Histochemistry. Analyt. Biochem. 149: 1–28.PubMedGoogle Scholar
  20. Cooke HJ, Schmidtke T and Gosden JR (1982) Characterisation of a human Y chromosome repeated sequence and related sequences in higher primates. Chromosoma 87: 491–502.PubMedGoogle Scholar
  21. Cordell JL, Fallini B, Erber WN, Ghosh AN, Abdulaziz Z, MacDonald S, Pulford KAF, Stein H and Mason DY (1984) Immunoenzymatic labelling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal antialkaline phosphatase. J. Histochem. Cytochem. 32: 219–229.PubMedGoogle Scholar
  22. Croen KD, Ostrove JM, Dragovic LJ and Straus SE (1988) Patterns of gene expression and sites of latency in human nerve ganglia are different for varicella-zoster and herpes simplex viruses. Proc. Natl. Acad. Sci. USA 85: 9773–9777.PubMedGoogle Scholar
  23. Crum CP, Nuovo G, Friedman D and Silverstein SJ (1988) Accumulation of RNA homologous to human papillomavirus type 16 open reading frames in genital precancers. J. Virol. 62: 84–90.PubMedGoogle Scholar
  24. Crum CP and Roche JK (1990) Molecular pathology of the lower female genital tract. The papillomavirus model. Am. J. Surg. Pathol. 14(Suppl 1): 26–33.PubMedGoogle Scholar
  25. Cubie HA and Norval M (1989) Detection of human papilloma viruses in paraffin wax sections with biotinylated synthetic oligonucleotide probes and immunogold staining. J. Clin. Pathol. 42: 988–991.PubMedGoogle Scholar
  26. Eilbott DJ, Peress N, Burger H, LaNeve D, Ornstein J, Gendelman HE, Seidman R and Weiser B (1989) Human immunodeficiency virus tupe 1 in spinal cords of acquired immunodeficiency patients with myelopathy: expression and replication in macrophages. Proc. Natl. Acad. Sci. USA 86: 3337–3341.PubMedGoogle Scholar
  27. Fleming KA (1987) In-situ hybridization — a role in clinical pathology (Editorial). J. Pathol. 153: 201–202.PubMedGoogle Scholar
  28. Fleming KA, Evans M, Riley C, Franklin D, Morey A and Lovell-Badge R (1991) High sensitivity of non-isotopic in situ hybridization using digoxigenin labelled probes and transgenic mice. J. Pathol. 163: 154A.Google Scholar
  29. Fox JD, Briggs M, Ward PA and Tedder RS (1990) Human herpesvirus 6 in salivary gl ands. Lancet 336: 590–593.PubMedGoogle Scholar
  30. Gendelman HE, Narayan O, Stoskopf-Kennedy S, Kennedy PG, Ghotbi Z, Clements JE, Stanley J and Pezeshkpour G (1986) Tropism of sheep lentiviruses for monocytes: susceptibility to infection and virus gene expression increase during maturation of monocytes to macrophages. J. Virol. 58: 67–74.PubMedGoogle Scholar
  31. Gendelmen HE, Moench TR, Narayan O, Griffin DE and Clements JE (1985) A double label technique for performing simultaneous immunocytochemistry and in situ hybridization in virus infected cell cultures and tissues. J. Virol. Meth. 11: 93–103.Google Scholar
  32. Goldfarb J (1989) Leads from the MMWR. Risks associated with parvovirus B19 infection. J. Am. Med. Assoc. 261: 1406–81555, 1560, 1563.Google Scholar
  33. Grody WW, Cheng LS and Lewin KJ (1987) In situ viral DNA hybridization in diagnostic surgical pathology. Hum. Pathol. 18: 535–543.PubMedGoogle Scholar
  34. Grody WW, Lewin KJ and Naeim F (1988) Detection of cytomegalovirus DNA in classic and epidemic Karposi’s sarcoma by in situ hybridization. Hum. Pathol. 19: 524–528.PubMedGoogle Scholar
  35. Haase AT, Stowring L, Ventura P, Burks J, Ebers G, Tourtelotte W and Warren K (1984) Detection by hybridization of viral infection of the human central nervous system. Ann. NY Acad. Sci. 436: 103–108.PubMedGoogle Scholar
  36. Haase AT, Walker D, Stowring L, Ventura P, Geballe A, Blum H, Brahic M, Goldberg R and O’Brien K (1985a) Detection of two viral genomes in single cells by double-label hybridization in situ and color microradioautography. Science 227: 189–191.PubMedGoogle Scholar
  37. Haase A, Gantz D, Eble B, Walker D, Stowring L, Ventura P, Blum H, Wietgrefe S, Zupancic M, Tourtellotte W, Gibbs C J, Norrby E and Rosenblatt S (1985b) Natural history of restricted synthesis and expression of measles virus genes in subacute sclerosing panencephalitis. Proc. Natl. Acad. Sci. USA 82: 3020–3024.PubMedGoogle Scholar
  38. Haase AT, Gantz D, Blum H, Stowring L, Ventura P, Geballe A, Moyer B and Brahic M (1985c) Combined macroscopic and microscopic detection of viral genes in tissues. Virology 140: 201–206.PubMedGoogle Scholar
  39. Haase A (1986) Analysis of viral infections by in situ hybridization. J. Histochem. Cytochem. 34: 27–32.PubMedGoogle Scholar
  40. Haase AT, Retzel EF and Staskus KA (1990) Amplification and detection of lentiviral DNA inside cells. Proc. Natl. Acad. Sci. USA 87: 4971–4975.PubMedGoogle Scholar
  41. Hadchouel M, Pasquinelli C, Fournier JG, Hugon RN, Scotto J, Bernard O and Brechot C (1988) Detection of mononuclear cells expressing hepatitis B virus in peripheral blood from HBsAg positive and negative patients by in situ hybridisation. J. Med. Virol. 24: 27–32.PubMedGoogle Scholar
  42. Hamilton-Dutoit S, Pallesen G, Karkov J, Skinhoj P, Franzmann MB and Pedersen C (1989) Identification of EBV-DNA in tumour cells of AIDS-related lymphomas by in-situ hybridisation [letter]. Lancet 1: 554–555.PubMedGoogle Scholar
  43. Härders J, Lukacs N, Robert-Nicoud M, Jovin TM and Riesner D (1989) Imaging of viroids in nuclei from tomato leaf tissue by in situ hybridization and confocal laser scanning microscopy. EMBO J. 8: 3941–3949.PubMedGoogle Scholar
  44. Hawkins EP, Krischer JP, Smith BE, Hawkins HK and Finegold MJ (1990) Nasopharyngeal carcinoma in children — a retrospective review and demonstration of Epstein-Barr viral genomes in tumor cell cytoplasm: a report of the Pediatric Oncology Group. Hum. Pathol. 21: 805–810.PubMedGoogle Scholar
  45. Henderson A, Ripley S, Heller M and Kieff E (1983) Chromosome site for Epstein-Barr virus DNA in a Burkitt tumor cell line and in lymphocytes growth transformed in vitro. Proc. Natl. Acad. Sci. USA 80: 1987–1991.PubMedGoogle Scholar
  46. Herrington CS, Burns J, Graham AK, Bhatt B and McGee JO’D (1989a) Interphase cytogenetics using biotin and digoxigenin labelled probes II: simultaneous differential detection of two nucleic acid species in individual nuclei. J. Clin. Pathol. 42: 601–606.PubMedGoogle Scholar
  47. Herrington CS, Graham AK, Flannery DMJ, Burns J and McGee JO’D (1990) Discrimination of closely homologous HPV types by nonisotopic in situ hybridization: definition and derivation of tissue melting temperatures. Histochem. J. 22: 545–554.PubMedGoogle Scholar
  48. Hofler H (1987) What’s new in “in situ hybridization”. Pathol. Res. Pract. 182: 421–430.PubMedGoogle Scholar
  49. Hogg JC, Irving WL, Porter H, Evans M, Dunnill MS and Fleming K (1989) In situ hybridization studies of adenoviral infections of the lung and their relationship to follicular bronchiectasis. Am. Rev. Respir. Dis. 139: 1531–1535.PubMedGoogle Scholar
  50. Jilbert AR, Freiman JS, Gowans EJ, Holmes M, Cossart YE and Burrell C (1987) Duck hepatitis B virus DNA in liver, spleen, and pancreas: analysis by in situ and Southern blot hybridization. Virology 158: 330–338.PubMedGoogle Scholar
  51. Jones JF, Shurin S, Abramowsky C, Tubbs RR, Sciotto CG, Wahl R, S ands J, Gottman D, Katz BZ and Sklar J (1988) T-cell lymphomas containing Epstein-Barr viral DNA in patients with chronic Epstein-Barr virus infections. N. Engl. J. Med. 318: 733–741.PubMedGoogle Scholar
  52. Kennedy PG, Adams JH, Graham DI and Clements G (1988) A clinico-pathological study of herpes simplex encephalitis. Neuropathol. Appl. Neurobiol. 14: 395–415.PubMedGoogle Scholar
  53. Koenig S, Gendelman HE, Orenstein JM, Canto MCD, Pezeshkpour GH, Yungbluth M, Janotta F, Aksamit A, Martin MA and Fauci A (1986) Detection of AIDS virus in macrophages in brain tissue from AIDS patients with encephalopathy. Science 233: 1089–1093.PubMedGoogle Scholar
  54. Lawrence JB, Villnave CA and Singer RH (1988) Sensitive, high-resolution chromatin and chromosome mapping in situ: presence and orientation of two closely integrated copies of EBV in a lymphoma line. Cell 52: 51–61.PubMedGoogle Scholar
  55. Lawrence JB, Singer RH and Marselle LM (1989) Highly localized tracks of specific transcripts within interphase nuclei visualized by in situ hybridization. Cell 57: 493–502.PubMedGoogle Scholar
  56. Lawrence JB, Marselle LM, Byron KS, Johnson CV, Sullivan JL and Singer RH (1990) Subcellular localization of low-abundance human immunodeficiency virus nucleic acid sequences visualized by fluorescence in situ hybridization. Proc. Natl. Acad. Sci. USA 87: 5420–5424.PubMedGoogle Scholar
  57. Lew andowski G, Delgado G, Holloway RW, Farrell M, Jenson AB and Lancaster WD (1990) The use of in situ hybridization to show human papillomavirus deoxyribonucleic acid in metastatic cancer cells within lymph nodes. Am. J. Obstet. Gynecol. 163: 1333–1337.PubMedGoogle Scholar
  58. Lewis SH, Reynolds-Kohler C, Fox HE and Nelson JA (1990) HIV-1 in trophoblastic and villous Hofbauer cells, and haematological precursors in eight-week fetuses. Lancet 335: 565–568.PubMedGoogle Scholar
  59. Lipkin WI, Villarreal LP and Oldstone MBA (1989) Whole animal section in situ hybridization and protein blotting: new tools in molecular analysis of animal models for human disease. Curr. Top. Microbiol. Immunol. 143: 33–54.PubMedGoogle Scholar
  60. Lo Y-MD, Mehal WZ and Fleming KA (1988) Rapid production of vector-free biotinylated probes using the polymerase chain reaction. Nuc. Acids Res. 16: 8719.Google Scholar
  61. Lohr JM and Oldstone MBA (1990) Detection of cytomegalovirus nucleic acid sequences in pancreas in type 2 diabetes. Lancet 336: 644–648.PubMedGoogle Scholar
  62. Maitl and NJ, Kinross JH, Busuttil A, Ludgate SM, Smart GE and Jones KW (1981) The detection of DNA tumor virus-specific RNA sequences in abnormal human cervical biopsies by in situ hybridization. J. Gen. Virol. 55: 123–137.PubMedGoogle Scholar
  63. Maitl and NJ, Cox MF, Lynas C, Prime S, Crane I and Scully C (1987) Nucleic acid probes in the study of latent viral disease. J. Oral. Pathol. 16: 199–211.PubMedGoogle Scholar
  64. Masih AS, Linder JL, Shaw BWJr, Wood RP, Donovan JP, White R and Markin RS (1988) Rapid identification of cytomegalovirus in liver allograft biopsies by in situ hybridization. Am. J. Surg. Pathol. 12: 362–367.PubMedGoogle Scholar
  65. McDougall JK, Dunn AR and Jones KW (1972) In situ hybridization of adenovirus RNA and DNA. Nature 236: 346.PubMedGoogle Scholar
  66. McDougall JK, Fenoglio CM and Galloway DA (1980) Cervical carcinoma: detection of herpes simplex virus RNA in cells undergoing neoplastic change. Int. J. Cancer 25: 1–8.PubMedGoogle Scholar
  67. McDougall JK, Myerson D and Beckmann AM (1986) Detection of viral DNA and RNA by in situ hybridization. J. Histochem. Cytochem. 34: 33–38.PubMedGoogle Scholar
  68. Milde K and Loning T (1986) Detection of papillomavirus DNA in oral papillomas and carcinomas: application of in situ hybridization with biotinylated HPV 16 probes. J. Oral. Pathol. 15: 292–296.PubMedGoogle Scholar
  69. Moen PTJr, Fox E and Bodnar JW (1990) Adenovirus and minute virus of mice DNAs are localized at the nuclear periphery. Nuc. Acids Res. 18: 513–519.Google Scholar
  70. Moench TR (1987) In situ hybridization (review). Mol. Cell. Probes 1: 195–205.PubMedGoogle Scholar
  71. Morey AL, Fleming KA, del-Buono R and Ch andler JA (1991a) A flexible method for non-isotopic in situ labelling of multiple nucleic acid and antigenic targets in individual cells or sections. J. Pathol. 163: 159A.Google Scholar
  72. Morey L, Fleming KA, Keeling JW and Porter HJ (1991b) Diagnosis and investigation of human fetal parvovirus infection by in situ hybridization combined with immunophenotyping of infected cells (submitted for publication)..Google Scholar
  73. Morey AL, Fleming KA, Ferguson D and Sutton L (1991c) Cellular features of parvovirus infection in vitro. J. Pathol. 163: 168A.Google Scholar
  74. Mullink H, Walboomers JM, Raap AK and Meyer CJ (1989a) Two colour DNA in situ hybridization for the detection of two viral genomes using non-radioactive probes. Histochemistry 91: 195–198.PubMedGoogle Scholar
  75. Mullink H, Walboomers JMM, Tadema TM, Jansen D and Meijer CJLM (1989b) Combined immuno-and non-radioactive hybridocytochemistry on cells and tissue sections: influence of fixation, enzyme pretreatment, and choice of chromogen on detection of antigen and DNA sequences. J. Histochem. Cytochem. 37: 603–609.PubMedGoogle Scholar
  76. Myerson D, Hackman RC, Nelson JA, Ward DC and McDougall JK (1984) Widespread presence of histologically occult cytomegalovirus. Hum. Pathol. 15: 430–439.PubMedGoogle Scholar
  77. Myerson D (1988) In Situ Hybridizaton. In: Diagnostic Immunopathology. (R. B. Colvin, A. K. Bhan and R. T. McCluskey eds.),pp 475–498 New York: Raven Press.Google Scholar
  78. Naoumov NV, Alex ander GJ, Eddieston AL and Williams R (1988a) In situ hybridisation in formalin fixed, paraffin wax embedded liver specimens: method for detecting human and viral DNA using biotinylated probes. J. Clin. Pathol. 41: 793–798.PubMedGoogle Scholar
  79. Naoumov NV, Alex ander GJM, O’Grady JG, Aldis P, Portman BC and Williams R (1988b) Rapid diagnosis of cytomegalovirus infection by in-situ hybridisation in liver grafts. Lancet 1: 1361–1364.PubMedGoogle Scholar
  80. Negro F, Bonino F, di Bisceglie A, Hoofnagle JH and Gerin JL (1989) Intrahepatic markers of hepatitis delta virus infection: a study by in situ hybridization. Hepatology 10: 916–920.PubMedGoogle Scholar
  81. Norval M and Bingham RW (1987) Advances in the use of nucleic acid probes in diagnosis of viral diseases of man. Arch. Virol. 97: 151–165.PubMedGoogle Scholar
  82. Orth G, Jeanteur P and Croissant O (1970) Evidence for and localization of vegetative viral DNA replication by autoradiographic detection of RNA-DNA hybrids in sections of tumors induced by Shope papilloma virus. Proc. Natl. Acad. Sci. USA 68: 1876–1880.Google Scholar
  83. Ozden S, Aubert C, Gonzalez-Dunia D and Brahic M (1990) Simultaneous in situ detection of two mRNAs in the same cell using riboprobes labeled with biotin and 35S. J. Histochem. Cytochem. 38: 917–922.PubMedGoogle Scholar
  84. Padel AF, Venning VA, Evans MF, Quantrill AM and Fleming KA (1990) Human papillomaviruses in anogenital warts in children: typing by in situ hybridisation. Br. Med. J. 300: 1491–1494.Google Scholar
  85. Patel D, Shepherd PS, Naylor JA and McCance DJ (1989) Reactivities of polyclonal and monclonal antibodies raised to the major capsid proteins of human papillomavirus type 16. J. Gen. Virol. 70: 69–77.PubMedGoogle Scholar
  86. Peluso R, Haase AT, Stowring L, Edwards M and Ventura P (1985) A Trojan Horse mechanism for the spread of visna virus in monocytes. Virology 147: 231–236.PubMedGoogle Scholar
  87. Perlman S, Jacobsen G and Afifi A (1989) Spread of a neurotropic murine coronavirus into the CNS via the trigeminal and olfactory nerves. Virology 170: 556–560.PubMedGoogle Scholar
  88. Pezzella M, Rossi P, Lombardi V, Gemelli V, Mariani-Costantini R, Mirolo M, Funaro C, Moschese V and Wigzell H (1989) HIV viral sequences in seronegative people at risk detected by in situ hybridization and polymerase chain reaction. Br. Med. J. 298: 713–716.Google Scholar
  89. Popescu NC and DiPaolo JA (1990) Integration of human papillomavirus 16 DNA and genomic rearrangements in immortalized human keratinocyte lines. Cancer Res. 50: 1316–1323.PubMedGoogle Scholar
  90. Porter HJ, Khong TY, Evans MF, Chan VT and Fleming KA (1988) Parvovirus as a cause of hydrops fetalis: detection by in situ DNA hybridisation. J. Clin. Pathol. 41: 381–383.PubMedGoogle Scholar
  91. Porter HJ, Heryet A, Quantrill AM and Fleming KA (1990) Combined non-isotopic in-situ hybridization and immunohistochemistry on routine paraffin wax embedded tissue: identification of cell type infected by human parvovirus and demonstration of cytomegalovirus DNA and antigen in renal infection. J. Clin. Pathol. 43: 129–132.PubMedGoogle Scholar
  92. Puvion-Dutilleul F and Puvion E (1989) Ultrastructural localization of viral DNA in thin sections of herpes simplex virus type 1 infected cells by in situ hybridization. Eur. J. Cell. Biol. 49: 99–109.PubMedGoogle Scholar
  93. Puvion-Dutilleul F, Pichard E, Laithier M and Puvion E (1989) Cytochemical study of the localization and organization of parental herpes simplex virus type I DNA during initial infection of the cell. Eur. J. Cell. Biol. 50: 187–200.PubMedGoogle Scholar
  94. Salimans MM, van de Rijke FM, Raap AK and van Elsacker-Niele AM (1989) Detection of parvovirus B19 DNA in fetal tissues by in situ hybridization and polymerase chain rection. J. Clin. Pathol. 42: 525–530.PubMedGoogle Scholar
  95. Stevens JG (1989) Herpes simplex virus latency analyzed by in situ hybridization. Curr. Top. Microbiol. Immunol. 143: 1–8.PubMedGoogle Scholar
  96. Stowring L, Haase AT, Petursson G, Georgsson G, Palsson P, Lutley R, Roos R and Szuchet S (1985) Detection of visna virus antigens and RNA in glial cells in foci of demyelination. Virology 141: 311–318.PubMedGoogle Scholar
  97. Syrjänen KJ (1987) Biology of human papillomavirus (HPV) infections and their role in squamous cell carcinogenesis. Med. Biol. 65: 21–39.PubMedGoogle Scholar
  98. Syrjänen S, Cintorino M, Armellini D, delVecchio MT, Leoncini P, Bugnoli M, Pallini V, Silvestri S, Tosi P, Mantyjarvi R, et al. (1988) Expression of cytokeratin polypeptides in human papillomavirus (HPV) lesions of the uterine cervix: 1. Relationship to grade of CIN and HPV type. Int. J. Gynecol. Pathol. 7: 23–38.Google Scholar
  99. Teo CG and Griffin B (1987) Epstein-Barr virus genomes in lymphoid cells: activation in mitosis and chromosomal location. Proc. Natl. Acad. Sci. USA 84: 8473–8477.PubMedGoogle Scholar
  100. Terry RM, Lewis FA, Robertson S, Blythe D and Wells M (1989) Juvenile and adult laryngeal papillomata: classification by in-situ hybridization for human papillomavirus. Clin. Otolaryngol. 14: 135–139.PubMedGoogle Scholar
  101. Turtinen LW, Saltzman R, Jordan MC and Haase A (1987) Interactions of human cytomegalovirus with leukocytes in vivo: analysis by in situ hybridization. Microb. Pathog. 3: 287–297.PubMedGoogle Scholar
  102. Unger ER and Brigati DJ (1989) Colorimetric in-situ hybridization in clinical virology: development of automated technology. Curr. Top. Microbiol. Immunol. 143: 21–31.PubMedGoogle Scholar
  103. Vallejos H, del Mistro A, Kleinhaus S, Braunstein JD, Halwer M and Koss LG (1987) Characterization of human papilloma virus types in condylomata acuminata in children by in situ hybridization. Lab. Invest. 56: 611–615.PubMedGoogle Scholar
  104. van der Loos CM, Volkers HH, Rook R, van den Berg FM and Houthoff H-J (1989) Simultaneous application of in situ DNA hybridization and immunohistochemistry on one tissue section. Histochem. J. 21: 279–284.PubMedGoogle Scholar
  105. Vazeux R, Cumont M, Girard PM, Nassif X, Trotot P, Marche C, Matthiessen L, Vedrenne C, Mikol J, Henin D, et al. (1990) Severe encephalitis resulting from coinfections with HIV and JC virus. Neurology 40: 944–948.PubMedGoogle Scholar
  106. Venables PJ, Teo CG, Baboonian C, Griffin BE and Hughes R (1989) Persistence of Epstein-Barr virus in salivary gl and biopsies from healthy individuals and patients with Sjogren’s syndrome. Clin. Exp. Immunol. 75: 359–364.PubMedGoogle Scholar
  107. Weiss LM, Movahed LA, Warnke RA and Sklar J (1989) Detection of Epstein-Barr viral genomes in Reed-Sternberg cells of Hodgkin’s disease. N. Engl. J. Med. 320: 502–506.PubMedGoogle Scholar
  108. Wolber RA and Lloyd RV (1988) Cytomegalovirus detection by nonisotopic in situ DNA hybridization and viral antigen immunostaining using a two-color technique. Hum. Pathol. 19: 736–741.PubMedGoogle Scholar
  109. Wolber RA, Beals TF, Lloyd RV and Maassab H (1988) Ultrastructural localization of viral nucleic acid by in situ hybridization. Lab. Invest. 59: 144–151.PubMedGoogle Scholar
  110. Wolber RA, Beals TF and Maassab H (1989) Ultrastructural localization of herpes simplex virus RNA by in situ hybridization. J. Histochem. Cytochem. 37: 97–104.PubMedGoogle Scholar
  111. Wolf BC, Martin AW, Neiman RS, Janckila AJ, Yam LT, Caracansi A, Leav BA, Winpenny R, Schultz DS and Wolfe HJ (1990) The detection of Epstein-Barr virus in hairy cell leukemia cells by in situ hybridization. Am. J. Pathol. 136: 717–723.PubMedGoogle Scholar
  112. Yamashiroya HM, Ghosh L, Yang R and Robertson ALJ (1988) Herpesviridae in the coronary arteries and aorta of young trauma victims. Am. J. Pathol. 130: 71–79.PubMedGoogle Scholar

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© Springer Science+Business Media Dordrecht 1992

Authors and Affiliations

  • Adrienne L. Morey
    • 1
  • Kenneth A. Fleming
    • 1
  1. 1.University of Oxford, Nuffield Department of Pathology and BacteriologyJohn Radcliffe HospitalHeadington, Oxford

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