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Electron Crystallography of Membrane Proteins

  • Conference paper
Membrane Proteins: Structures, Interactions and Models

Part of the book series: The Jerusalem Symposia on Quantum Chemistry and Biochemistry ((JSQC,volume 25))

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Abstract

The technical capabilities of electron diffraction and high resolution electron microscopy have recently been advanced to the point that three-dimensional density maps can be obtained at sufficiently high resolution to allow an interpretation in terms of the structure at atomic resolution. The high scattering intensity of electrons compared to x-rays results in the fact that two-dimensional crystals, one molecule thick, are ideal specimens for electron crystallography. In the event that large, well ordered, three-dimensional crystals cannot be obtained, which are required for x-ray diffraction, electron diffraction now represents a practical alternative for high resolution structure analysis. The conditions needed to obtain two-dimensional crystals are, in general, very different from those which can produce three-dimensional crystals. As a result, attempts to obtain two-dimensional crystals complement the more traditional approach, which would be to obtain crystals for x-ray crystallography.

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Author information

Authors and Affiliations

  1. Department of Molecular and Cell Biology Stanley/Donner ASU, University of California, Berkeley, CA, 94720, USA

    R. M. Glaeser

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  1. R. M. Glaeser
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Editors and Affiliations

  1. Institut de Biologie Physico-Chimique, Fondation Edmond de Rothschild, Paris, France

    Alberte Pullman  & Bernard Pullman  & 

  2. Department of Chemistry, University of Tel-Aviv, Israel

    Joshua Jortner

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© 1992 Springer Science+Business Media Dordrecht

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Glaeser, R.M. (1992). Electron Crystallography of Membrane Proteins. In: Pullman, A., Jortner, J., Pullman, B. (eds) Membrane Proteins: Structures, Interactions and Models. The Jerusalem Symposia on Quantum Chemistry and Biochemistry, vol 25. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2718-9_1

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  • DOI: https://doi.org/10.1007/978-94-011-2718-9_1

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-5205-4

  • Online ISBN: 978-94-011-2718-9

  • eBook Packages: Springer Book Archive

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