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Transformation of E.Coli by Electroporation and Expression of Lac Z Gene

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Recent Advances in Biotechnology

Part of the book series: NATO ASI Series ((NSSE,volume 210))

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Abstract

The insertion of functional DNA molecules into cells by electropermeabilization is a powerfull tool for transformation (1). Under suitable conditions the electric field causes permeabilization of cell membrane to certain molecules refferred as electroporation (2). In recent studies the technique was used to transfer DNA, RNA, protein, and small molecules like flourescent dyes, nucleotides, durgs, cations into cells (3). In several cases the transformation by electroporation provides a significant advance over chemical means (4), even it may be the only transformation method for certain cell types (5).

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References

  1. Teissie, J., Rols, M.P. (1985). Biotehnology, 3, 1987.

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  2. Neumann, E., Schaeffer-Ridder, M., Wong, Y., Hofshneider, P.H. (1982). EMBO J., 1, 841–845.

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  3. Shigekawa, K., Dower, W.J. (1988). Biotechniques, 6(8), 742–751.

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  4. Anderson, G.L., Evans, A.G. (1988).Biotechniques, 6(7), 650–660.

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  5. Mercenier, A., Chassy, B.M. (1988). Boichimie, 70, 503–507.

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© 1992 Springer Science+Business Media Dordrecht

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Gündüz, U., Eren, E. (1992). Transformation of E.Coli by Electroporation and Expression of Lac Z Gene. In: Vardar-Sukan, F., Sukan, Ş.S. (eds) Recent Advances in Biotechnology. NATO ASI Series, vol 210. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2468-3_40

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  • DOI: https://doi.org/10.1007/978-94-011-2468-3_40

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-5089-0

  • Online ISBN: 978-94-011-2468-3

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