Abstract
The insertion of functional DNA molecules into cells by electropermeabilization is a powerfull tool for transformation (1). Under suitable conditions the electric field causes permeabilization of cell membrane to certain molecules refferred as electroporation (2). In recent studies the technique was used to transfer DNA, RNA, protein, and small molecules like flourescent dyes, nucleotides, durgs, cations into cells (3). In several cases the transformation by electroporation provides a significant advance over chemical means (4), even it may be the only transformation method for certain cell types (5).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Teissie, J., Rols, M.P. (1985). Biotehnology, 3, 1987.
Neumann, E., Schaeffer-Ridder, M., Wong, Y., Hofshneider, P.H. (1982). EMBO J., 1, 841–845.
Shigekawa, K., Dower, W.J. (1988). Biotechniques, 6(8), 742–751.
Anderson, G.L., Evans, A.G. (1988).Biotechniques, 6(7), 650–660.
Mercenier, A., Chassy, B.M. (1988). Boichimie, 70, 503–507.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1992 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Gündüz, U., Eren, E. (1992). Transformation of E.Coli by Electroporation and Expression of Lac Z Gene. In: Vardar-Sukan, F., Sukan, Ş.S. (eds) Recent Advances in Biotechnology. NATO ASI Series, vol 210. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2468-3_40
Download citation
DOI: https://doi.org/10.1007/978-94-011-2468-3_40
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-5089-0
Online ISBN: 978-94-011-2468-3
eBook Packages: Springer Book Archive