Abstract
Starch gel electrophoresis was first described by Smithies (1955). Isoenzymes and alloenzymes were separated electrophoretically by starch gel electrophoresis. Besides other biochemical markers isozymes became a useful tool for different applications in plant breeding in the last few years. For example, isozyme analysis have been used to describe and identify varieties, which can’t be distinguished by morphological markers only; to obtain genetic structures of populations; to check genetic purity of hybrid seeds. Here, it will be describe the electrophoretic procedure used for identification of genotypes and genetic purity control of hybrid seeds.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Smithies, O., (1955) ‘Zone electrophoresis in starch gels’. Biochem. J. 61, 629–641.
Stuber, G.W., Goodman, M.M., and Johnson, F.M. (1977) ‘Genetic control and radical variation of B-Glucosidase isozymes in maize’. Biochem. Genetics. 15, 383–394.
Vallejos, E. (1983) ‘Enzyme activity staining’, in S.D. Tanksley and T.J.Orton (eds.). Isozymes in plant genetics and breeding. Amsterdam. Elsevier. 469-516.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1992 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Bilgen, G., Demir, İ. (1992). Isoenzyme Electrophoresis on Maize. In: Vardar-Sukan, F., Sukan, Ş.S. (eds) Recent Advances in Biotechnology. NATO ASI Series, vol 210. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2468-3_38
Download citation
DOI: https://doi.org/10.1007/978-94-011-2468-3_38
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-5089-0
Online ISBN: 978-94-011-2468-3
eBook Packages: Springer Book Archive