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Transformation of Streptococcus Thermophilus with Plasmid DNA by Electroporation

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Recent Advances in Biotechnology

Part of the book series: NATO ASI Series ((NSSE,volume 210))

Abstract

Electroporation is the application of high voltage electric field pulses to induce transient pores in the cell membrane through which the diffusion and exchange of molecules can occur. The plasmid pMK3 which contains Lac Z gene was introduced into the industrial yogurt Streptococcus thermophilus by electroporation. The early logarithmic phase cells had the highest transformability. The optimum electric field strength was 6 kV/cm. The number of transformats increased with plasmid DNA concentration. ß-Galactosidase activity of Streptococcus thermophilus was increased 7.5 fold after the electrotarnsformation. Electroporation is a simple and effective technique for genetic manipulation.

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Author information

Authors and Affiliations

  1. Biology Department, Middle East Technical University, Ankara, 06531, Turkey

    Ufuk Gündüz, Işin Geren & Emel Eren

  2. Food Engineering Department, Middle East Technical University, Ankara, 06531, Turkey

    Fatih Yildiz

  3. Chemical Engineering Department, Middle East Technical University, Ankara, 06531, Turkey

    Güngör Gündüz

Authors
  1. Ufuk Gündüz
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  2. Işin Geren
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  3. Emel Eren
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  4. Fatih Yildiz
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  5. Güngör Gündüz
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Editor information

Editors and Affiliations

  1. Department of Chemical Engineering, Ege University, Izmir, Turkey

    Fazilet Vardar-Sukan

  2. Biotechnology Centre and Department of Food Engineering, Ege University, Izmir, Turkey

    Ş. Suha Sukan

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© 1992 Springer Science+Business Media Dordrecht

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Gündüz, U., Geren, I., Eren, E., Yildiz, F., Gündüz, G. (1992). Transformation of Streptococcus Thermophilus with Plasmid DNA by Electroporation. In: Vardar-Sukan, F., Sukan, Ş.S. (eds) Recent Advances in Biotechnology. NATO ASI Series, vol 210. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2468-3_17

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  • DOI: https://doi.org/10.1007/978-94-011-2468-3_17

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-5089-0

  • Online ISBN: 978-94-011-2468-3

  • eBook Packages: Springer Book Archive

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