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Plant Genomes: Methods for Genetic and Physical Mapping pp 141–165Cite as

Construction and application of a YAC library

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Abstract

As the desire to map genomes has grown over the last decade, the technical means have grown along with it. With the development of different cloning vectors, pulsed-field gel electrophoresis (PFGE) [15,16,19,46 and van Daelen and Zabel, this book], jumping libraries [21,39] and the polymerase chain reaction (PCR) [43], projects on genome mapping are possible for almost all species. The mapping strategies may be broadly grouped into two general classes, genetic and physical mapping. These two approaches yield qualitatively different information, e.g., genetic maps allow one to get an overall view of an entire chromosome with low resolution of details, whereas physical maps provide very high resolution in local regions of a chromosome without necessarily linking distant regions. The two mapping approaches therefore complement each other, and genome mapping projects including both strategies have good possibilities of being successful.

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Author information

Authors and Affiliations

  1. Centre d’Etude du Polymorphisme Humain, 27, rue Juliette Dodu, 75010, Paris, France

    Hans M. Albertsen, Hadi Abderrahim, Daniel Cohen & Denis Le Paslier

Authors
  1. Hans M. Albertsen
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  2. Hadi Abderrahim
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  3. Daniel Cohen
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  4. Denis Le Paslier
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Editor information

Editors and Affiliations

  1. Department of Plant Genetics and Breeding, Agricultural Research Organization, The Volcani Center, Bet-Dagan, Israel

    J. S. Beckmann

  2. Department of Agronomy, University of Wisconsin, Madison, WI, USA

    T. C. Osborn

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© 1992 Springer Science+Business Media Dordrecht

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Albertsen, H.M., Abderrahim, H., Cohen, D., Le Paslier, D. (1992). Construction and application of a YAC library. In: Beckmann, J.S., Osborn, T.C. (eds) Plant Genomes: Methods for Genetic and Physical Mapping. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2442-3_7

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  • DOI: https://doi.org/10.1007/978-94-011-2442-3_7

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-5077-7

  • Online ISBN: 978-94-011-2442-3

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