Abstract
This chapter describes all of the techniques required for inserting a foreign coding sequence into a baculovirus transfer vector and preparing DNA which is suitable for performing co-transfection experiments to derive recombinant virus. Most of the techniques presented are not unique to the baculovirus expression system, and the reader who is experienced with molecular biology may not necessarily agree with all of our protocols. The methods in this chapter, however, represent the everyday procedures used in our own laboratories and are presented in the knowledge that they do work. Alternative and additional procedures may be gleaned from the excellent laboratory manuals edited by Berger and Kimmel (1987) and Sambrook et al. (1989).
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© 1992 L. A. King and R. D. Possee
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King, L.A., Possee, R.D. (1992). Construction of transfer vectors containing the foreign gene. In: The Baculovirus Expression System. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2374-7_4
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DOI: https://doi.org/10.1007/978-94-011-2374-7_4
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-5047-0
Online ISBN: 978-94-011-2374-7
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