Probiotics pp 355-376 | Cite as

Probiotics for humans



This chapter will review the current status of probiotics in the area of human health and nutrition, including some reference of the use of probiotics in foods. The development of new strains of bacteria that can either improve current medical use or be used for medical problems in which probiotics have not been previously considered applicable or effective will be discussed. In this regard a review of both selective procedures and bio engineering techniques will be discussed.


Lactic Acid Bacterium Hepatic Encephalopathy Fermented Milk Intestinal Microflora Lactobacillus Acidophilus 
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  1. Alm, L. (1982a) Effect of fermentation on lactose, glucose and galactose content in milk and suitability of fermented milk products far lactose individuals. J. Dairy Sci., 65, 346–52.CrossRefGoogle Scholar
  2. Alm, L. (1982b) Effect of fermentation on B-vitamin content of milk in Sweden. J. Dairy Sci., 65, 353–9.CrossRefGoogle Scholar
  3. Alm, L. (1983) The effect of Lactobacillus acidophilus administration upon the survival of Salmonella in randomly selected human carriers. Prog. Food Nutr. Sci., 7, 13–17.Google Scholar
  4. Axelsson, L., Chung, T.C., Dobrogosz, W.J. and Lindgren, L.E. (1987) Discovery of a new antimicrobial substance produced by Lactobacillus reuteri. FEMS Microbial Rev. 46 (3), 65.Google Scholar
  5. Bennett, R.G., Garbach, S.L., Goldin, B.R. et al. (1991), Treatment of relapsing Clostridium difficile diarrhea with lyophilyzed Lactobacillus GG. Submitted far publication.Google Scholar
  6. Bogdanov, L.G., Velichkov, V.T. and Gurvich, A.L (1978) Antitumor action of glycopeptide from the cell wall of Lactobacillus bulgaricus. Bull. Exp. Biol. Med., 84, 1750–3.CrossRefGoogle Scholar
  7. Breslaw, E.S. and Kleyn, D.H. (1973) In vitro digestibility of protein in yogurt at various stages of processing. J. Food Sci., 38, 1016–21.CrossRefGoogle Scholar
  8. Broussalian, J. and Westhoff, D. (1983) Influence of lactose concentration on milk and yogurt growth of rats. J. Dairy Sci., 66, 438–43.CrossRefGoogle Scholar
  9. Bruce, E.R., Varhese, A.J., Furrer, R. and Land, P.C. (1977) A mutagen in the feces of normal humans, in Origins of Human Cancer, Cold Spring Harbor Lab, New York.Google Scholar
  10. Chassy, B.M. (1987) Prospects for the genetic manipulation of lactobacilli. FEMS Microbiol. Rev., 46 (3), 197–312.Google Scholar
  11. Clements, M.L., Levine, M.M. and Ristaino, P.A. (1983) Exogenous lactobacili fed to man: Their fate and ability to prevent diarrheal disease. Prog. Food Nutr., 7, 29–37.Google Scholar
  12. Conway, P.L., Gorbach, S.L. and Goldin, B.R. (1987) Survival of lactic acid bacteria in the human stomach and adhesion to intestinal cells. J. Dairy Sci., 70, 1–12.CrossRefGoogle Scholar
  13. Conway, P.L. and Kjelleberg, S. (1989) Protein-mediated adhesion of Lactobacillus fermentum strain 737 to mouse stomach squamous epithelium. J. Gen. Microbiol., 135, 1175–86.Google Scholar
  14. Deeth, H.C. and Tamine, A.Y. (1981) Yogurt: nutritive and therapeutic aspects. J. Food Protect., 44, 78–86.Google Scholar
  15. Deneke, C.F, McGowan, K., Thorne, G.M. and Gorbach, S.L. (1983) Attachment of entertoxigenic Eschericha coli to human intestinal cells. Infect. Immun., 39, 1102–6.Google Scholar
  16. De Simone, C., Salvatori, B.B., Jirillo, E. et al. (1989a) Modulation of immune activities in humans and animals by dietary lactic acid bacteria, in Yogurt: Nutritional and Health Properties, National Yogurt Assoc., McLean, Virginia.Google Scholar
  17. De Simone, C., Salvatori, B.B., Jirillo, E. et al. (1989b) Yogurt and the immune response, in Les Laits Fermentes, John Libbey, London.Google Scholar
  18. De Vos, W.M. (1987) Gene cloning and expression in lactic streptococci. FEMS Microbiol. Rev. 46 (3), 281–95.Google Scholar
  19. De Wit, O., Pochart, P. and Desjeux, J.F. (1988) Breath hydrogen concentration and plasma glucose, insulin and free fatty acid levels after lactose, milk, fresh or heated yogurt ingestion by healthy young adults with or without lactose malabsorption. Nutrition, 4, 131–5.Google Scholar
  20. Drasar, B.S. and Hill, M.J. (1974) Human Intestinal Flora, Academic Press, New York.Google Scholar
  21. Drasar, B.S., Shiner, M. and McLeod, G.H. (1969) Studies on the intestinal flora. 1. The bacterial flora of the gastrointestinal tract in health and achlorhydric persons. Gastroenterology, 56, 71–9.Google Scholar
  22. Ellis-Pegler, R.B., Crabtree, C. and Lambert, H.P. (1975) The faecal flora of children in the United Kingdom. J. Hyg. (Camb), 75, 135–42.CrossRefGoogle Scholar
  23. Fernandes, C.F., Shahani, K.M. and Amer, M.A. (1987) Therapeutic role of dietary lactobacilli and lactobacillic fermen ted dairy products. FEMS Microbiol. Rev., 46 (3), 343–56.CrossRefGoogle Scholar
  24. Finegold, S.M., Atterbery, H.R. and Sutter, V.L. (1974) Effect of diet on human fecal flora: Comparison of Japanese and American diets. Amer. J. Clin. Nutr., 27, 1546–9.Google Scholar
  25. Friend, B.A., Farmer, R.E. and Shahani, K.M. (1982) Effect of feeding and intraperitoneal implantation of yogurt culture cells on Ehrlich ascites tumor. Milchwiss., 37, 708–10.Google Scholar
  26. Friend, B.A. and Shahani, K.M. (1984) Nutritional and therapeutic aspects of lactobacilli. J. Appl. Nutr., 36, 125–53.Google Scholar
  27. Gilliland, S.E., Nelson, C.R. and Maxwell, C. (1985) Assimilation of cholesterol by Lactobacillus acidophilus. Appl. Environ. Microbiol. 49, 377–81.Google Scholar
  28. Goldin, B.R. and Gorbach, S.L. (1977) Alterations in fecal microflora enzymes related to diet, age, lactobacillus supplements, and dimethylhydrazine. Cancer, 40, 2421–6.CrossRefGoogle Scholar
  29. Goldin, B.R. and Gorbach, S.L. (1980) Effect of Lactobacillus acidophilus dietary supplementation on 1,2-dimethylhydrazine dihydrochloride-induced intestinal cancer in rats. J. Natl Cancer Inst., 64, 263–5.Google Scholar
  30. Goldin, B.R. and Gorbach, S.L. (1981) The effect of antibiotics on rat intestinal tumors induced by dimethylhydrazine. J. Natl Cancer Inst., 67, 877–80.Google Scholar
  31. Goldin, B.R. and Gorbach, S.L. (1984a) Effect of milk and Lactobacillus feeding on human intestinal bacterial enzyme activity. Amer J. Clin. Nutr., 39, 756–61.Google Scholar
  32. Goldin, B.R. and Gorbach, S.L. (1984b) Alterations of the intestinal microflora by diet, oral antibiotics and Lactobacillus. Decreased production of free amines from aromatic nitro compounds, azo dyes and glucuronides. J. Natl Cancer Inst., 73, 689–95.Google Scholar
  33. Goldin, B.R. and Gorbach, S.L. (1987) Lactobacillus GG: A new strain with properties favorable for survival, adhesion and antimicrobial activity in the gastrointestinal tract. FEMS Microbiol. Rev., 46 (3), 72.Google Scholar
  34. Gorbach, S.L., (1990) Lactic acid bacteria and human health. Ann. Med., 22, 37–41CrossRefGoogle Scholar
  35. Gorbach, S.L., Chang, T.W. and Goldin, B.R. (1987) Successful treatment of relapsing Clostridium difficile colitis with Lactobacillus GG. Lancet, ii, 1519.CrossRefGoogle Scholar
  36. Gotz, V., Romankiewicz, J.A., Moss, J. and Murray, H.W. (1979) Prophylaxis against ampicillin-associated diarrhea with a lactobacillus preparation. Amer. J. Hosp. Pharmacol., 36, 754.Google Scholar
  37. Goulet, J., Saucier, L. and Maineau, S. (1989) Stimulation of the non-specific immune response of mice by fermented milks, in Yogurt: Nutritional and Health Properties, National Yogurt Assoc., McLean, Virginia.Google Scholar
  38. Grunewald, K.K. (1982) Serum cholesterol levels in rats fed skim milk fermented by Lactobacillus acidophilus. J. Food Sci., 47, 2078–9.Google Scholar
  39. Grutte, F.K., Horn, R. and Haenel, H. (1965) Ernahrung und biochemisch-mikrookologische Vorgange in Enddarm von Sauglingen. Z. Kinderh., 93, 28–39.CrossRefGoogle Scholar
  40. Haenel, H. (1970) Human normal and abnormal gastrointestinal flora. Amer. J. Clin. Nutr., 23, 1433–9.Google Scholar
  41. Haenel, H. and Benedig, J. (1975) Intestinal flora in health and disease. Prog. Food Nutr. Sci., 1, 21–64Google Scholar
  42. Hargrove, R.E. and Alford, J.A. (1978) Growth rate and feed efficiency of rats fed yogurt and other fermented milks. J. Dairy Sci., 61, 11–19.CrossRefGoogle Scholar
  43. Hargrove, R.E. and Alford, J.A. (1980) Growth response of weanling rats to heated, aged, fractionated, and chemically treated yogurts. J. Dairy Sci., 63, 1065–72.CrossRefGoogle Scholar
  44. Hepner, G., Fried, R., St Jean, S. et al. (1979) Hypocholestermic effect of yogurt and milk. Amer. J. Clin. Nutr., 32, 19–24.Google Scholar
  45. Hitchins, A.D., Wells, P., McDonough, F.E. and Wong, N.A. (1985) Amelioration of the adverse effect of a gastrointestinal challenge with Salmonella enteritidis on weanling rats by a yogurt diet. Amer. J. Clin. Nutr., 41, 91–100.Google Scholar
  46. Kilara, A. and Shahani, K.M. (1976) Lactose activity of cultured and acidified dairy products. J. Dairy Sci., 61, 2031–5.CrossRefGoogle Scholar
  47. Kim, H.S. and Gilliland, S.E. (1983) Lactobacillus acidophilus as a dietary adjunct for milk to aid lactose digestion in humans. J. Dairy Sci., 66, 959–66.CrossRefGoogle Scholar
  48. Kimmey, M.B., Eimer, G.W., Surawicz, C.M. and McFarland, I.V. (1990) Prevention of further recurrences of Clostridium difficile colitis with Saccharomyces boulardii. Digest Dis. Sci., 35, 897–901.CrossRefGoogle Scholar
  49. Kleeman, E.G. and Klaenhammer, T.R. (1982) Adherence of Lactobacillus species to human intestinal cells. J. Dairy Sci., 65, 2063–9.CrossRefGoogle Scholar
  50. Kolars, I.C., Levitt, M.D., Aouji, M. and Savaino, D.A. (1984) Yogurt-an autodigesting source of lactose. New Engl. J. Med., 310, 1–3.CrossRefGoogle Scholar
  51. Leavitt, R.I. and Ryan, F.X. (1974) L-Lysine decarboxylase as a specific inhibitor of growth of a species Pichia. J. Gen. Microbiol., 80, 311–13.CrossRefGoogle Scholar
  52. Macbeth, W.A.A.G., Kass, K.H. and McDermott, W.V. Jr(1965) Treatment of hepatic encephalopathy by alteration of intestinal flora with Lactobacillus acidophilus. Lancet, i, 399–403.CrossRefGoogle Scholar
  53. Mann, G.V. and Spoerig, A. (1974) Studies of a surfactant and cholesteraemia in masai. Amer. J. Glin. Nutr., 27, 464–9.Google Scholar
  54. McDonough, F.K, Hitchins, A.D. and Wong, N.P. (1982) Effects of yogurt and freeze dried yogurt on growth stimulation of rats. J. Food Sci., 47, 1463–5.CrossRefGoogle Scholar
  55. McDonough, F.K Wells, P., Wong, N.P. et al. (1983) Role of vitamins and minerals in growth stimulation of rats fed yogurt. Fed. Proc., 42, 556.Google Scholar
  56. McDonough F.K, Hitchins, A.D., Wong, N.P. et al. (1987) Modification of sweet acidophilus milk to improve utlization by lactose-intolerant persons. Amer. J. Glin. Nutr., 45, 570–4.Google Scholar
  57. Moore, W.K.C. and Holdeman, L.V. (1974) Human fecal flora: The normal flora of 20 Japanese-Hawaiians. Appl. Microbiol., 27, 961–79.Google Scholar
  58. Niv, M., Levy, W. and Greenstein, N.M. (1963) Yogurt in the treatment of infantile diarrhea. Glin. Pediat. (Phila), 2, 407–11.CrossRefGoogle Scholar
  59. Oksanen, P.J., Salminen, S., Saxelin, M. et al. (1990) Prevention of travellers diarrhea by Lactobacillus GG. Ann. Med., 22, 53–6.CrossRefGoogle Scholar
  60. Perdigón, S., Alvarez, S., Noder de Macias, M.K and Medici, M. (1989) Effect of lactic acid bacteria orally administered and of yoghurt on the immune system, in Les Laits Fermentes, John Libby, London.Google Scholar
  61. Pulsani, S.R. and Rao, D.R. (1983) Whole body liver and plasma cholesterol levels in rats fed thermophilnus, bulgaricus and acidophilus milks. J. Foud Sci., 48, 280–1CrossRefGoogle Scholar
  62. Rajalakshmi, R. and Vanaji, K. (1967) Chemical and biological evaluation of the effects of fermentation on the nutritive value of foods prepared from rice and grain. Brit. J. Nutr., 21, 467–73.CrossRefGoogle Scholar
  63. Rao, D.M., Ghawan, C.B., and Pulusani, S.R (1981) Influence of milk and thermophilie milk plasma cholesterol levels and hepatic cholesterogenesis in rats. J. Food Sci., 46, 1339–41.CrossRefGoogle Scholar
  64. Read, A.K, McGarthy, G.F., Heaton, K.W., and Laidlow, J. (1966) Lactobacillus acidophilus (ENPAC) in treatment of hepatic encephalopathy. Brit. Med J., 1, 1267–9.CrossRefGoogle Scholar
  65. Reddy, G.V., Friend, B.A, Shahani, K.M, and Farmer, R.K. (1983) Antitumor activity of yogurt components J. Food Protect., 46 8–11.Google Scholar
  66. Robins-Browne, R.M. and Levine, M. (1981) The fate of ingested lactobacilli in the proximal small intestine. Amer J. Clin. Nutr., 34, 514–19.Google Scholar
  67. Rotimi, V.O. and Duerden, B.I. (1981). The development of the bacterial flora in normal neonates. J. Med. Microbiol., 34 51–62.CrossRefGoogle Scholar
  68. Sands, D.C. and Hankin, L. (1974) Selecting lysine-excreting mutants of lactobacilli for used in food and feed enrichment. Appl. Microbiol., 28, 523–4.Google Scholar
  69. Sands, D.C. and Hankin, L. (1976) Fortification of foods by fermentation with lysine-excreting mutants of lactobacilli. J. Agric. Food Chem., 24, 1104–6.CrossRefGoogle Scholar
  70. Savaiano, D.A., Abouelanouar, A., Smith, D.K and Levitt, M.O. (1984) Lactose malabsorption from yogurt, pasteurized yogurt, sweet acidophilus milk, and cultured milk in lactose-deficient individuals. Amer J. Clin. Nutr., 40, 1219–23.Google Scholar
  71. Scheline, R.R. (1973) Metabolism of foreign compounds by gastrointestinal microorganisms. Pharmocol. Rev., 25, 451–523.Google Scholar
  72. Scheline, R.R. (1980) Drug metabolism by the gastrointestinal microflora. Monogr. Pharmacol. Physiol., 5, 551–80.Google Scholar
  73. Siitonen, S., Vapoatalo, H., Salminen, S. et al. (1990) Effect of Lactobacillus GG yoghurt in prevention of antibiotic-associated diarrhoea. Ann. Med., 22, 57–9.CrossRefGoogle Scholar
  74. Silva, M., Jacobus, N.V., Deneke, C. and Gorbach, S.L. (1987) Antimicrobial substance from a human Lactobacillus strain. Antimicrob. Agents Chemother., 31, 1231–3.CrossRefGoogle Scholar
  75. Shahani, K.M., and Chandan, R.C. (1979) Nutritional and healthful aspects of cultured and culture-containing dairy foods. J. Dairy Sci., 62, 1685–94.CrossRefGoogle Scholar
  76. Shahani, K.M., Friend, B.A. and Bailey, P.J. (1983), Antitumor activity of fermented colostrum and milk. J. Food Protect., 46, 385–6.Google Scholar
  77. Stark, P.L. and Lee, A. (1982) The microbial ecology of the large bowel of breast-fed and formula-fed infants during the first year of life. J. Med. Microbial., 15, 189–203.CrossRefGoogle Scholar
  78. Surawicz, C.M., Eimer, G.W., Speelman, P. et al. (1989a) Prevention of antibiotic-associated diarrhea by Saccharomyces boulardii: A prospective study. Gastroenterolgy, 96, 981–8.Google Scholar
  79. Surawicz, C.M., McFarland, L.V., Eimer, G. and Chinn, J. (1989b) Treatment of recurrent Clostridium difficile colitis with vancomycin and Saccharomyces boulardii. Amer. J. Gastroenterol., 84, 1285–7.Google Scholar
  80. Zychowicz, C., Surazynmska, A., Sietwierska, B. and Ciephinska, T. (1974) Effect of Lactobacillus acidophilus cultures (acidophilus milk) on the carrier state of shigella and salmonella organisms in children. Pediatria Polska, 49, 997–1003.Google Scholar
  81. Zychowicz, C., Kowalczyk, S. and Ciephinska, T. (1975) Results of administration of Lactobacillus acidophilus cultures (acidophilus milk) in an endemie focus of dysentery. Pediatria Polska, 50, 429–35.Google Scholar

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