Abstract
Molecular biological tools currently available to us are revolutionizing the way in which we can address questions in evolutionary biology. The purpose of this article is to provide an overview of molecular techniques and applications available to biologists who are interested in evolutionary studies but who have little acquaintance with molecular biology. In evolutionary biology, techniques designed to determine degree of nucleic acid similarity are in common use and will be dealt with first. Another approach, namely gene expression studies, has strong implications for evolutionary biology but generally requires substantial familiarity with molecular biological tools. Expression studies provide powerful tools for discerning processes of speciation, as in the selection of genetic variants, as well as discerning lineages, e.g., expression of specific homeobox genes during segment formation. For investigations where either nucleic acid identity or gene expression are the ultimate goal, detailed information, protocols and appropriate controls are beyond the scope of this work but, where possible, recent review articles are cited.
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Ferraris, J.D. (1993). Molecular approaches to the study of evolution and phylogeny of the Nemertina. In: Gibson, R., Moore, J., Sundberg, P. (eds) Advances in Nemertean Biology. Developments in Hydrobiology, vol 89. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2052-4_19
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DOI: https://doi.org/10.1007/978-94-011-2052-4_19
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