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Molecular approaches to the study of evolution and phylogeny of the Nemertina

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Advances in Nemertean Biology

Part of the book series: Developments in Hydrobiology ((DIHY,volume 89))

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Abstract

Molecular biological tools currently available to us are revolutionizing the way in which we can address questions in evolutionary biology. The purpose of this article is to provide an overview of molecular techniques and applications available to biologists who are interested in evolutionary studies but who have little acquaintance with molecular biology. In evolutionary biology, techniques designed to determine degree of nucleic acid similarity are in common use and will be dealt with first. Another approach, namely gene expression studies, has strong implications for evolutionary biology but generally requires substantial familiarity with molecular biological tools. Expression studies provide powerful tools for discerning processes of speciation, as in the selection of genetic variants, as well as discerning lineages, e.g., expression of specific homeobox genes during segment formation. For investigations where either nucleic acid identity or gene expression are the ultimate goal, detailed information, protocols and appropriate controls are beyond the scope of this work but, where possible, recent review articles are cited.

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References

  • Avise, J. C., J. Arnold, R. M. Ball, E. Bermingham, T. Lamb, J. E. Neigel, C. A. Reeb & N. C. Saunders, 1987. Intraspecific phylogeography: the mitochondrial bridge between population genetics and systematics. Annu. Rev. Ecol. Syst. 18: 489–522.

    Google Scholar 

  • Avise, J. C., B. W. Bowen & T. Lamb, 1989. DNA fingerprints from hypervariable mitochondrial genotypes. Mol. Biol. Evol. 6: 258–269.

    CAS  PubMed  Google Scholar 

  • Bachmann, B., W. Luke & G. Hunsmann, 1990. Improvement of PCR amplified DNA sequencing with the aid of detergents. Nucleic Acids Res. 18: 1309.

    Article  CAS  PubMed  Google Scholar 

  • Belyaysky, A., T. Vinograova & K. Rajewsky, 1989. PCRbased cDNA library construction: general cDNA libraries at the level of a few cells. Nucleic Acids Res. 17: 2919–2932.

    Article  Google Scholar 

  • Britten, R. J., D. E. Graham & B. R. Neufeld, 1974. Analysis of repeating DNA sequences by reassociation. In L. Grossman & K. Moldave (eds), Methods in Enzymology. Academic Press, New York: 363–406.

    Google Scholar 

  • Brown, W. M., M. George Jr. & A. C. Wilson, 1979. Rapid evolution of animal mitochondrial DNA. Proc. Natn. Acad. Sci. U.S.A. 76: 1967–1971.

    Article  CAS  Google Scholar 

  • Brown, W. M., E. M. Prager, A. Wang & A. C. Wilson, 1982. Mitochondrial DNA sequences of primates: tempo and mode of evolution. J. Mol. Evol. 18: 225–239.

    Article  CAS  PubMed  Google Scholar 

  • Caccone, A., G. D. Amato & J. R. Powell, 1988. Rates and patterns of scnDNA and mtDNA divergence within the Drosophila melanogaster subgroup. Genetics 118: 671–683.

    CAS  PubMed  Google Scholar 

  • Cowley, B. D., Jr., J. D. Ferraris, D. Carper & M. B. Burg, 1990. In vivo osmoregulation of aldose reductase mRNA, protein, and sorbitol in renal medulla. Am. J. Physiol. 258: F154–F161.

    CAS  PubMed  Google Scholar 

  • Dowling, T. E., C. Moritz & J. D. Palmer, 1990. Nucleic acids II: restriction site analysis. In D. M. Hillis & C. Moritz (eds.), Molecular Systematics. Sinauer, Sunderland, Mass.: 250–317.

    Google Scholar 

  • Ferraris, J. D., 1984. Volume regulation in intertidal Procephalothrix spiralis (Nemertina) and Clitellio arenarius (Oligochaeta). J. Comp. Physiol. B 154: 125–137.

    Article  Google Scholar 

  • Ferraris, J. D., 1985. Effects of ablation of the cerebral organs and cerebral ganglia on volume regulation in an intertidal nemertine, Paranemertes Peregrina. Physiol. Zool. 58: 117–128.

    Google Scholar 

  • Ferraris, J. D. & H. K. Roderick, 1982. Involvement of ninhydrin positive substances in regulatory volume decrease in intertidal Nemertina and Oligochaeta: independence of de-cerebration. Mt. Desert Island Biol. Lab. Bull. 22: 33–35.

    Google Scholar 

  • Ferraris, J. D. & B. Schmidt-Nielsen, 1982. Volume regulation in an intertidal nemertine, Procephalothrix spiralis Coe. I. Short-term effects and independence of decerebration. J. exp. Zool. 224: 307–319.

    Article  Google Scholar 

  • Ferraris, J. D., B. D. Cowley, Jr., C. K. Williams, B. M. Martin, M. B. Burg & A. Garcia-Perez, 1990a. Rabbit aldose reductase, an osmoregulatory protein is encoded by a single gene. FASEB J. 4: A1823.

    Google Scholar 

  • Ferraris, J. D., C. K. Williams, B. M. Martin, M. B. Burg & A. Garcia-Perez, 1990. Cloning and structure of the rabbet aldose reductase gene. J. am. Soc. Nephrol. 1: 739.

    Google Scholar 

  • Field, K. G., G. J. Olsen, D. J. Lane, S. J. Giovannoni, M. T. Ghiselin, E. C. Raff, N. R. Pace & R. A. Raff, 1988. Molecular phylogeny of the animal kingdom. Science 239: 748–753.

    Article  CAS  PubMed  Google Scholar 

  • Garcia-Perez, A., B. Martin, H. R. Murphy, S. Uchida, H. Murer, B. D. Cowley, J. S. Handler & M. B. Burg, 1989. Molecular cloning of cDNA coding for kidney aldose reductase: Regulation of specific mRNA accumulation by NaCl-mediated osmotic stress. J. Biol. Chem. 264: 16815–16821.

    CAS  PubMed  Google Scholar 

  • Gyllensten, U. B. & H. A. Erlich, 1988. Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DOA locus. Proc. natn. Acad. Sci. U.S.A. 85: 7652–7656.

    Article  CAS  Google Scholar 

  • Harrison, R. G., 1989. Mitochondrial DNA as a genetic marker in population and evolutionary biology. Trends Ecol Evol. 4: 6–11.

    Article  CAS  PubMed  Google Scholar 

  • Hendriks, L., E. Huysmans, A. Vandenberghe & R. De Wachter, 1986. Primary structures of the 5S ribosomal RNAs of 11 arthropods and applicability of 5S RNA to the study of metazoan evolution. J. Mol. Evol. 24: 103–109.

    Article  CAS  Google Scholar 

  • Kocher, T. D., W. K. Thomas, A. Meyer, S. V. Edwards, S. Paabo, F. X. Villablanca & A. C. Wilson, 1989. Dynamics of mitochondrial DNA evolution in animals: amplification and sequencing with conserved primers. Proc. Natl. Acad. Sci. U.S.A. 86: 6196–6200.

    Article  CAS  PubMed  Google Scholar 

  • Lake, J. A., 1990. Origin of the metazoa. Proc. natn. Acad. Sci. U.S.A. 87: 763–766.

    Article  CAS  Google Scholar 

  • Lane, D. J., B. Pace, G. J. Olsen, D. A. Stahl, M. L. Sogin & N. R. Pace, 1985. Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. Proc. natn. Acad. Sci. U.S.A. 82: 6955–6959.

    Article  CAS  Google Scholar 

  • Lee, C. C., X. W. Wu, R. A. Gibbs, R. G. Cook, D. M. Muzny & C. T. Caskey, 1988. Generation of cDNA probes directed by amino acid sequence: cloning of urate oxidase. Science 239: 1288–1291.

    Article  CAS  PubMed  Google Scholar 

  • Li, W-H. & D. Graur, 1991. Fundamentals of molecular evolution. Sinauer, Sunderland, Mass. U.S.A.

    Google Scholar 

  • Medlin, L., H. J. Elwood, S. Stickel & M. L. Sogin, 1988. The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions. Gene 71: 491–499.

    Article  CAS  PubMed  Google Scholar 

  • Mullis, K. B., F. Faloona, S. J. Scharf, R. K. Saiki, G. T. Horn & H. A. Erlich, 1986. Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction. Cold Spring Harbor Symp. Quant. Biol. 51: 263–273.

    Article  CAS  PubMed  Google Scholar 

  • Pierce, S. K., L. R. Faux & S. M. O’Brien, 1990. Salinity tolerance differences between Chesapeake Bay and Atlantic oysters: differences in amino acid and glycine betaine metabolism. Am. Physiol. Soc.: A-37.

    Google Scholar 

  • Prosser, C. L., 1990. Comparative physiology: theory of adaptation. In C. L. Prosser (ed.) Environmental and metabolic animal physiology, comparative animal physiology, 4th edition. Wiley-Liss, New York: 1–11.

    Google Scholar 

  • Qu, L. H., B. Michot & J. P. Bachellerie, 1983. Improved methods for structure probing in large RNAs: a rapid ‘heterologous’ sequencing approach is coupled to the direct mapping of nuclease accessible sites. Application to the 5’ terminal domain of eucaryotic 28S rRNA. Nucleic Acids Res. 11: 5903–5920.

    Article  CAS  PubMed  Google Scholar 

  • Qu, L. H., M. Nicoloso & J. P. Bachellerie, 1986. Phylogenetic calibration of the 5’ terminal domain of large rRNA achieved by determining twenty eucaryotic sequences. J. Mol. Evol. 28: 113–124.

    Article  Google Scholar 

  • Robey, R., M. Kwon, J. Handler, A. Garcia-Perez & M. Burg, 1991. Induction of glycinebetaine uptake into Xenopus oocytes by injection of poly(A)+RNA from renal cells exposed to high extracellular NaCl. J. Biol. Chem. 266: 10400–10405.

    CAS  PubMed  Google Scholar 

  • Saccone, D., G. Pesole & E. Sbisa, 1991. The main regulatory region of mammalian mitochondrial DNA: structure-function model and evolutionary patter. J. Mol. Evol. 33: 83–91.

    Article  CAS  PubMed  Google Scholar 

  • Sanger, F., S. Nicklen & A. R. Coulson, 1977. DNA sequencing with chain-terminating inhibitors. Proc. natn. Acad. Sci. U.S.A. 74: 5463–5467.

    Article  CAS  Google Scholar 

  • Saiki, R. K., S. J. Scharf, F. A. Faloona, K. B. Mullis, G. T. Horn, H. A. Erlich & N. Arnheim, 1985. Enzymatic amplification of ß-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 230: 1350–1354.

    Article  CAS  PubMed  Google Scholar 

  • Saiki, R. K., D. H. Gelfand, S. Stoffel, R. Higuchi, G. T. Horn, K. B. Mullis & H. A. Erlich, 1988. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239: 487–491.

    Article  CAS  PubMed  Google Scholar 

  • Smardo, F. M., Jr., M. B. Burg & A. Garcia-Perez, 1990. Os-motic regulation of kidney aldose reductase (AR) gene transcription. FASEB J. 4: A2096.

    Google Scholar 

  • Springer, M. & C. Krajewski, 1989. DNA hybridization in animal taxonomy: a critique from first principles. Quart. Rev. Biol. 64: 291–318.

    Article  CAS  PubMed  Google Scholar 

  • Syvanen, A. C., K. Aalto-Setala, K. Kontula & H. Soderlund, 1989. Direct sequencing of affinity-captured amplified human DNA application to the detection of apolipoprotein E polymorphism. FEB S Letters 258: 71–74.

    Article  CAS  Google Scholar 

  • Uchida, S., A. Garcia-Perez, H. Murphy & M. B. Burg, 1989. Signal for induction of aldose reductase in renal medullary cells by high external NaCl. Am. J. Physiol. 256: C614–C620.

    CAS  PubMed  Google Scholar 

  • Uchida, S., M. Kwon, A. Preston & J. Handler, 1991. Expression of MDCK cell Na+ and Cl-dependent taurine transporter in Xenopus laevis oocytes. J. Biol. Chem. 266: 9605–9609.

    CAS  PubMed  Google Scholar 

  • Upholt, W. B., 1977. Estimation of DNA sequence divergence from comparison of restriction endonuclease digests. Nucleic Acids Res. 4: 1257–1265.

    Article  CAS  PubMed  Google Scholar 

  • Vawter, L. & W. M. Brown, 1986. Nuclear and mitochondrial DNA comparisons reveal extreme rate variation in the molecular clock. Science 234: 194–196.

    Article  CAS  PubMed  Google Scholar 

  • Werman, S. D., M. S. Springer & R. J. Britten, 1990. Nucleic acids I: DNA-DNA hybridization. In D. M. Hillis & C. Moritz (eds), Molecular Systematics. Sinauer, Sunderland: 204–249.

    Google Scholar 

  • White, T. J., T. Bruns, S. Lee & J. Taylor, 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In M. A. Innis, D. H. Gelfand, J. J. Sninsky & T. J. White (eds), PCR protocols: a guide to methods and applications. Academic Press, N.Y.: 315–321.

    Google Scholar 

  • Wilson, A. C., 1976. Gene regulation in evolution. In F. J. Ayala (ed.), Molecular Evolution. Sinauer, Sunderland Mass., U.S.A.: 225–234.

    Google Scholar 

  • Wilson, A. C., R. L. Cann, S. M. Carr, M. George, U. B. Gyllensten, K. M. Helm-Bychowski, R. G. Higuchi, S. R. Palumbi, E. M. Prager, R. D. Sage & M. Stoneking, 1985. Mitochondrial DNA and two perspectives on evolutionary genetics. Biol. J. linn. Soc. 26: 375–400.

    Article  Google Scholar 

  • Wilson, A. C., E. A. Zimmer, E. M. Prager & T. D. Kocher, 1989. Restriction mapping in the molecular systematics of mammals: a retrospective salute. In B. Fernholm, K. Bremer & H. Jornvall (eds), The hierarchy of life. Elsevier, Amsterdam: 407–419.

    Google Scholar 

  • Wrischnik, L. A., R. G. Higuchi, M. Stoneking, H. A. Erlich, N. Arnheim & A. C. Wilson, 1987. Length mutations in human mitochondrial DNA: direct sequencing of enzymatically amplified DNA. Nucleic Acids Res. 15: 529–542.

    Article  CAS  PubMed  Google Scholar 

  • Xiong, B. & T. D. Kocher, 1991. Comparison of mitochondria) DNA sequences of seven morphospecies of black flies (Diptera: Simulidae). Genome 34: 306–311.

    Article  CAS  PubMed  Google Scholar 

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R. Gibson J. Moore P. Sundberg

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© 1993 Springer Science+Business Media Dordrecht

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Ferraris, J.D. (1993). Molecular approaches to the study of evolution and phylogeny of the Nemertina. In: Gibson, R., Moore, J., Sundberg, P. (eds) Advances in Nemertean Biology. Developments in Hydrobiology, vol 89. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2052-4_19

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  • DOI: https://doi.org/10.1007/978-94-011-2052-4_19

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-4909-2

  • Online ISBN: 978-94-011-2052-4

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