Abstract
Pure phloem sap was collected from insects feeding on rice (Oryza sativa L.) leaves by a laser technique similar to the aphid stylet technique. Rapid circulation of nitrogen in the sieve tubes was demonstrated directly using 15N as a tracer. Application to the roots of the metabolic inhibitors of amino acids, aminooxyacetate and methioninesulfoximine, changed the amino acid composition in the sieve tubes. Feeding methionine to leaf tips resulted in its bulk transfer into the sieve tubes.
In vitro experiments confirmed the existence of protein kinases in the pure rice phloem sap. The phosphorylation status of the sieve tube sap proteins was affected by the light regime. The possibility that changes in chemical composition or protein modification such as phosphorylation in the sieve tubes might affect plant growth are discussed.
Analysis of pure phloem sap collected from rice plants by insect laser technique has shown dynamic changes in the chemical composition and the quality of proteins in the sap.
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References
Hayashi H and Chino M 1990 Plant Cell Physiol. 31, 247–251.
Kumazawa K and Goleb J A 1969 Plant Cell Physiol. 10, 725–731.
Nakamura S, Hayashi H and Chino M 1990 Abst. International conference of phloem transport and assimilate compartmentation. pp 13 Cognac (France).
Mae T and Ohira K 1981 Plant Cell Physiol. 22, 1067–1074.
Pate J S and Atkins C A 1983 Plant Physiol. 71, 835–840.
Purse J G 1984 J. Exp. Bot. 35, 227–238.
Yoneyama T and Takeba G 1984 Plant Cell Physiol. 25, 39–48.
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© 1993 Springer Science+Business Media Dordrecht
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Hayashi, H., Nakamura, S., Ishiwatari, Y., Mori, S., Chino, M. (1993). Changes in the amino acid composition and protein modification in phloem sap of rice. In: Barrow, N.J. (eds) Plant Nutrition — from Genetic Engineering to Field Practice. Developments in Plant and Soil Sciences, vol 54. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-1880-4_33
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DOI: https://doi.org/10.1007/978-94-011-1880-4_33
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-4832-3
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