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Molecular characterization of cell populations in the maize root apex

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Molecular and Cell Biology of the Plant Cell Cycle

Abstract

Several features make the root apex of maize (Zea mays L.) a good experimental system for studying cell cycle controls in relation to development in higher plants. Actively dividing meristematic cells, slowly-cycling quiescent centre cells, differentiating cap cells and senescent detaching cap cells are distinctively compartmented. In addition, the quiescent centre can be activated into rapid proliferation in response to stresses. Although there is a wealth of cytological and physiological information about the behaviour of cells in the root apex, very little is known about the molecular factors which control the patterns of cell division and differentiation. Differential screening of cDNA libraries obtained from discrete cell populations from the apex may provide a means to identify genes which are expressed in cell cycle- and differentiation-dependent manners.

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References

  1. Akowitz A and Manuelidis L (1989) A novel cDNA /PCR strategy for efficient cloning of small amounts of undefined RNA. Gene 81: 295–306.

    Article  PubMed  CAS  Google Scholar 

  2. Barlow PW (1973) Mitotic cycles in root meristems. In: The Cell Cycle in Development and Differentiation. M Balls and FS Billet (eds) pp. 133–165. Cambridge University Press, Cambridge.

    Google Scholar 

  3. Barlow PW and Macdonald PDM (1973) An analysis of the mitotic cell cycle in the root meristem of Zea mays. Proc R Soc B 183: 385–398.

    Article  Google Scholar 

  4. Barlow PW (1974) Regeneration of the cap of primary roots of Zea mays. New Phytol 73: 937–954.

    Article  CAS  Google Scholar 

  5. Barlow PW (1977) The time-course of endoreduplication of nuclear DNA in the root cap of Zea mays. Cytobiologie 16: 98–105.

    CAS  Google Scholar 

  6. Barlow PW (1978) The concept of the stem cell in the context of plant growth and development. In: Stem Cells and Tissue Homeostasis. BI Lord, CS Potten and R Cole (eds) pp. 87–113. Cambridge University Press, Cambridge.

    Google Scholar 

  7. Barlow PW (1978) Cell displacement through the columella of the root cap of Zea mays L. Ann Bot 42: 791–799.

    Google Scholar 

  8. Barlow PW and Rathfelder EL (1984) Correlations between the dimensions of different zones of grass root apices, and their implications for morphogenesis and differentiation in roots. Ann Bot 53: 249–260.

    Google Scholar 

  9. Barlow PW (1992) The cell division cycle in relation to root organogenesis. Plant Growth Regulator Supplement (in press).

    Google Scholar 

  10. Belyavsky A, Vinogradova T and Rajeewsky K (1989) PCR-based cDNA library construction: general cDNA libraries at the level of a few cells. Nucleic Acid Res 17: 2919–2932.

    Article  PubMed  CAS  Google Scholar 

  11. Biermann B, Johnson EM and Feldman LJ (1990) Characterization and distribution of a maize cDNA encoding a peptide similar to the catalytic region of second messenger dependent protein kinases. Plant Physiol 94: 1609–1615.

    Article  PubMed  CAS  Google Scholar 

  12. Chojecki J (1986) Identification and characterization of a cDNA clone for histone H3 in barley. Carslberg Res Commun 51: 211– 217.

    Article  CAS  Google Scholar 

  13. Chomczynski P and Sacchi N (1987) Single step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162: 156–159.

    Article  PubMed  CAS  Google Scholar 

  14. Clowes FAL (1954) The promeristem and the minimal constructional centre in grass root apices. New Phytol 53: 108–116.

    Article  Google Scholar 

  15. Clowes FAL (1956) Nucleic acids in root apical meristems of Zea. New Phytol 55: 29–34.

    Article  CAS  Google Scholar 

  16. Clowes FAL (1961) Apical meristems. Blackwell Scientific Publications, Oxford.

    Google Scholar 

  17. Clowes FAL (1971) The proportion of cells that divide in root meristems of Zea mays L. Ann Bot 35: 249–261.

    Google Scholar 

  18. Clowes FAL (1972) The control of cell proliferation within root meristems. In: The Dynamics of Meristem Cell Populations. MW Miller and CC Kuehnert (eds) pp. 133–147. Plenum Publishing Corporation, New York.

    Chapter  Google Scholar 

  19. Clowes FAL (1975) The quiescent centre. In: The Development and Function of Roots. JG Torrey and DT Clarkson (eds) pp. 3–19. Academic Press, London.

    Google Scholar 

  20. Clowes FAL (1976) The root apex. In: Cell Division in Higher Plants. MM Yeoman (ed) pp. 253–284. Academic Press, London.

    Google Scholar 

  21. Clowes FAL (1978) Origin of the quiescent centre in Zea mays. New Phytol 80: 409–419.

    Article  Google Scholar 

  22. Denhardt DT, Edwards DR and Parfett CLS (1986) Gene expression during the mammalian cell cycle. Biochim Biophys Acta 865: 83–125.

    PubMed  CAS  Google Scholar 

  23. Domec C, Garbay B, Fournier M and Bonnet J. (1990) cDNA library construction from small amounts of unfractionated RNA: association of cDNA synthesis with polymerase chain reaction amplification. Anal Biochem 188: 422–426.

    Article  PubMed  CAS  Google Scholar 

  24. Feldman LJ and Torrey JG (1975) The quiescent center and primary vascular tissue pattern formation in cultured roots of Zea. Can J Bot 53: 2796–2803.

    Article  Google Scholar 

  25. Feldman LJ (1976) The de novo origin of the quiescent centre in regenerating root apices of Zea mays. Planta 128: 207–212.

    Article  Google Scholar 

  26. Feldman LJ and Torrey JG (1976) The isolation and culture in vitro of the quiescent center of Zea mays. Am J Bot 63: 345–355.

    Article  Google Scholar 

  27. Feldman LJ (1984) Regulation of root development. Annu Rev Plant Physiol 35: 223–242.

    Article  PubMed  CAS  Google Scholar 

  28. Fisher DB (1968) Localization of endogeneous RNA polymerase activity in frozen sections of plant tissues. J Cell Biol 39: 745–749.

    Article  PubMed  CAS  Google Scholar 

  29. Goyal V and Pillai A (1986) Anatomical and histochemical studies of root apical meristems of some angiosperms. Beitr Biol Pflanzen 62: 167–178.

    Google Scholar 

  30. Gurr SJ and McPherson MJ (1991) PCR-directed cDNA libraries. In: PCR: A Practical Approach. MJ McPherson, P Quirke and GR Taylor (eds) pp. 147–170. Oxford University Press, Oxford.

    Google Scholar 

  31. Jensen WA (1958) The nucleic acid and protein content of root tip cells of Vicia faba and Allium cepa. Exp Cell Res 14: 575–583.

    Article  PubMed  CAS  Google Scholar 

  32. Jepson I, Bray J, Jenkins G, Schuch W and Edwards K (1991) A rapid procedure for the construction of PCR cDNA libraries from small amounts of plant tissue. Plant Mol Biol Report 9: 131–138.

    Article  CAS  Google Scholar 

  33. Kodama H, Masaki I, Hattori T and Komamine A (1991) Isolation of genes that are preferentially expressed at the G1/S boundary during the cell cycle in synchronised cultures of Catharanthus roseus cells. Plant Physiol 95: 406–411.

    Article  PubMed  CAS  Google Scholar 

  34. McKinney JD and Heintz N (1991) Transcriptional regulation in the eukaryotic cell cycle. TIBS 16: 430–435.

    PubMed  CAS  Google Scholar 

  35. Osley MA (1991) The regulation of histone synthesis in the cell cycle. Annu Rev Biochem 60: 827–861.

    Article  PubMed  CAS  Google Scholar 

  36. Raghavan V and Olmedilla A (1989) Spatial patterns of histone mRNA expression during grain development and germination in rice. Cell Diff Dev 27: 183–196.

    Article  CAS  Google Scholar 

  37. Raghavan V (1990) Origin of the quiescent center in the root of Capsella bursa-pastoris (L.) Medik Planta 181: 62–70.

    Google Scholar 

  38. Romer AS and Parsons TS (1986) The vertebrate body plan. 6th edn. Saunders, Philadelphia.

    Google Scholar 

  39. Sabelli PA and Shewry PR (1991) Characterization and organization of gene families at the Gli-1 loci of bread and durum wheats by restriction fragment analysis. Theor Appl Genet 83: 209–216.

    Article  Google Scholar 

  40. Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT Mullis KB and Erlich HA (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239: 487–491.

    Article  PubMed  CAS  Google Scholar 

  41. Sargent T and Dawid I (1983) Differential gene expression in the gastrula of Xenopus laevis. Science 222: 135–139.

    Article  PubMed  CAS  Google Scholar 

  42. Steeves TA and Sussex IM (1989) Patterns in plant development. 2nd edn. Cambridge University Press, Cambridge.

    Book  Google Scholar 

  43. Torrey JG (1972) On the initiation of organization in the root apex. In: The Dynamics of Meristem Cell Populations. MW Miller and CC Kuehnert (eds) pp. 133–147. Plenum Publishing Corporation, New York.

    Google Scholar 

  44. Webster PL and Langenauer HD (1973) Experimental control of the activity of the quiescent centre in excised root tips of Zea mays. Planta 112: 91–100.

    Article  CAS  Google Scholar 

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Authors
  1. Paolo A. Sabelli
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  2. Shirley R. Burgess
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  3. Jesus V. Carbajosa
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  4. Jill S. Parker
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  5. Nigel G. Halford
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  6. Peter R. Shewry
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  7. Peter W. Barlow
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Editor information

Editors and Affiliations

  1. I.C.I. Agrochemicals, Jealott’s Hill Research Station, Bracknell, UK

    J. C. Ormrod

  2. University of Wales, College of Cardiff, UK

    D. Francis

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© 1993 Springer Science+Business Media Dordrecht

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Sabelli, P.A. et al. (1993). Molecular characterization of cell populations in the maize root apex. In: Ormrod, J.C., Francis, D. (eds) Molecular and Cell Biology of the Plant Cell Cycle. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-1789-0_8

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  • DOI: https://doi.org/10.1007/978-94-011-1789-0_8

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-4787-6

  • Online ISBN: 978-94-011-1789-0

  • eBook Packages: Springer Book Archive

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