Abstract
Histone H2A mRNA is expressed developmentally in specific cells in the pea root apical meristem. A double labelling technique was used to identify cells replicating DNA and also expressing H2A mRNA. We found that approximately 90% of the S-phase cells were expressing H2A mRNA. About 10% of the cycling cells expressed H2A mRNA outside of S-phase and about 10% of S-phase cells did not express H2A mRNA. When DNA synthesis was inhibited with hydroxyurea, a commensurate and specific decrease in steady state levels of H2A mRNA was found. We conclude that cell specific expression of pea histone H2A mRNA is dependent on cell cycle regulation and that H2A mRNA is transiently accumulated during a period of the cell cycle which mostly overlaps the S-phase. An assumption that H2A mRNA accumulation was implemented in late G1 , and abated in late S could account for this observation.
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Tanimoto, E.Y., Rost, T.L., Comai, L. (1993). Histone H2A expression during S-phase: histological co-localization of H2A mRNA and DNA synthesis in pea root tips. In: Ormrod, J.C., Francis, D. (eds) Molecular and Cell Biology of the Plant Cell Cycle. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-1789-0_7
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DOI: https://doi.org/10.1007/978-94-011-1789-0_7
Publisher Name: Springer, Dordrecht
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