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A p34cdc2-based cell cycle: its significance in monocotyledonous, dicotyledonous and unicellular plants

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Molecular and Cell Biology of the Plant Cell Cycle

Abstract

A requirement in proliferating cells for attainment of adequate cell size for progress through the cell cycle provides a means for coordinating rates of division with rates of growth. Control points of the plant cell cycle are in late Gl-phase and at the initiation of mitosis. A p34cdc2-based cell cycle in plants has been indicated by changes in the amount, phosphorylation and activity of p34cdc2-like protein in late G1 and at mitosis, and by association of plant p34cdc2 protein with two regulatory binding proteins. Plant p34cdc2 bound in vitro to p13sucl protein, which is necessary for progress through anaphase in fission yeast, and plant p34cdc2 co-purified with a cyclin B-like 56 kDa protein that was recognised by antibody against p56cdc13/cyclin B protein of fission yeast. A sharp peak in activity of p34cdc2 protein kinase correlated with the transient presence of cyclin B-like protein during normal mitosis. Moreover, in a metaphase-arresting mutant persistence of cyclin B protein correlated with persistence of activated p34cdc2 kinase activity and with inability to progress to anaphase under restrictive conditions. Selective inhibition of individual events at mitosis revealed that chromosome condensation, spindle formation, nuclear envelope breakdown and preprophase band disassembly occur independently following an initiating stimulus at prophase that coincides with, and probably derives from, the activation of p34cdc2 kinase. In development, the presence of p34cdc2-like protein was restricted to meristem tissues and to stages of organogenesis in which division is appropriate. During cell differentiation in diverse tissues, and probably universally, a more than fifteen fold reduction of p34cdc2 established a secure exit from the cell cycle and resumption of division required restoration of p34cdc2 levels. Both auxin and cytokinin phytohormones influenced the induced levels of the cell cycle control protein.

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Authors
  1. Peter C. L. John
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  2. Kerong Zhang
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  3. Chongmei Dong
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Editors and Affiliations

  1. I.C.I. Agrochemicals, Jealott’s Hill Research Station, Bracknell, UK

    J. C. Ormrod

  2. University of Wales, College of Cardiff, UK

    D. Francis

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© 1993 Springer Science+Business Media Dordrecht

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John, P.C.L., Zhang, K., Dong, C. (1993). A p34cdc2-based cell cycle: its significance in monocotyledonous, dicotyledonous and unicellular plants. In: Ormrod, J.C., Francis, D. (eds) Molecular and Cell Biology of the Plant Cell Cycle. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-1789-0_2

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  • DOI: https://doi.org/10.1007/978-94-011-1789-0_2

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