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Abstract

Although plant cell cultures offer unique advantages as an experimental system for cell cycle research, would-be users should be aware of the problems of culture-induced variation, traceable to a feed-forward cycle involving competitive selection and somatic cell cytogenetics. The most useful contribution of cell cultures has been to provide material with a high degree of synchrony for cell cycle research. Investigations of the control of the mitotic cycle by plant growth substances are bogged down in the slough of signal transduction, currently an area of ignorance. There is a pressing need for more evidence of causal connection between an early biochemical event induced by the signal and signal-induced progress through the cell cycle.

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References

  1. Anderson L and Wolter KE (1966) Cyclitols in plants: biochemistry and physiology. Annu Rev Plant Physiol 17: 209–222.

    Article  CAS  Google Scholar 

  2. Bender L and Neumann K-H (1978) Investigations on the influence of pre-culture in IAA-and kinetin-containing culture media on subsequent growth of cultured carrot explants. Zeits Pflanzenphysiol 88: 201–208.

    CAS  Google Scholar 

  3. Bevan M and Northcote DH (1981) Subculture-induced protein synthesis in tissue cultures of Glycine max and Phaseolus vulgaris. Planta 152: 24–31.

    Article  CAS  Google Scholar 

  4. Connett RJA and Hanke DE (1987) Changes in the pattern of phospholipid synthesis during the induction by cytokinin of cell division in soybean suspension cultures. Planta 170: 161–167.

    Article  CAS  Google Scholar 

  5. Ettlinger C and Lehle L (1988) Auxin induces rapid changes in phosphatidylinositol metabolites. Nature 331: 176–178.

    Article  PubMed  CAS  Google Scholar 

  6. Evans DA (1989) Somaclonal Variation -genetic basis and breeding applications. Trends Genet 5: 46–50.

    Article  PubMed  CAS  Google Scholar 

  7. Everett NP, Wang TL, Gould AR and Street HE (1981) Control of the cell cycle in cultured plant cells II. Effects of 2,4-D. Protoplasma 106: 15–22.

    Article  CAS  Google Scholar 

  8. Guri, A, Zelcer A and Izhar S (1984) Induction of high mitotic index in Petunia suspension cultures by sequential treatment with aphidicolin and colchicine. Plant Cell Reports 3: 219–221.

    Article  CAS  Google Scholar 

  9. Hanke DE, Davies, H, Biffen M, Connett RJA and Freathy TC (1990) Cytokinin mode of action-problems and perspectives. In: Plant Growth Substances (1988) RP Pharis and SB Rood (eds) pp. 161–172. Berlin: Springer-Verlag.

    Chapter  Google Scholar 

  10. Hata S, Kouchi H, Suzuka I and Ishii T (1991) Isolation and characterization of cDNA clones for plant cyclins. EMBO J 10: 2681–2688.

    PubMed  CAS  Google Scholar 

  11. Hirt H, Pay A, Gyorgyey J, Bako L, Nemeth K, Bogre L, Schweyen RJ, Heberle-Bors E and Dudits D (1991) Complementation of a yeast cell cycle mutant by an alfalfa cDNA encoding a protein kinase homologous to p34cdc2. Proc Natl Acad Sci USA 88: 1636–1640.

    Article  PubMed  CAS  Google Scholar 

  12. Ito M, Kodama H and Komamine A (1991) Identification of a novel S-phase-specific gene during the cell cycle in synchronous cultures of Catharanthus roseus cells. Plant J 1: 141–148.

    Article  PubMed  CAS  Google Scholar 

  13. Karlin-Neumann GA, Brusslan JA and Tobin EM (1991) Phytochrome control of the tms2 gene in transgenic Arabidopsis: A strategy for selecting mutants in the signal transduction pathway. Plant Cell 3: 573–582.

    PubMed  CAS  Google Scholar 

  14. Larkin PJ, Banks PM, Bhati R, Brettell RIS, Davies PA, Ryan SA, Scowcroft WR, Spindler LH and Tanner GJ (1989) From somatic variation to variant plants: mechanisms and applications. Genome 31: 705–711.

    Article  Google Scholar 

  15. Nagata T, Okada K and Takebe I (1982) Mitotic protoplasts and their infection with tobacco mosaic virus RNA encapsulated in liposomes. Plant Cell Reports 1: 250–252.

    Article  Google Scholar 

  16. Nakajima H, Yokota T, Matsumoto T, Noguchi M and Takahashi N (1979) Relationship between hormone content and autonomy in various autonomous tobacco cells cultured in suspension. Plant Cell Physiol 29: 1489–1499.

    Google Scholar 

  17. Parsons A, Blackford S and Sanders D (1989) Kinetin-induced stimulation of electrogenic pumping in soybean suspension cultures is unrelated to signal transduction. Planta 178: 215–222.

    Article  CAS  Google Scholar 

  18. Pfosser M (1989) Improved method for critical comparison of cell cycle data of asynchronously dividing and synchronized cell cultures of Nicotiana tabacum. J Plant Physiol 134: 741–745.

    Article  Google Scholar 

  19. Timblin C, Battey J and Kuehl WM (1990) Application of PCR technology to subtractive cDNA cloning. Nucleic Acids Res 6: 1587–1593.

    Article  Google Scholar 

  20. Trewavas A and Gilroy S (1991) Signal transduction in plant cells. Trends Genet 7: 356–361.

    PubMed  CAS  Google Scholar 

  21. Wang TL, Everett NP, Gould AR and Street HE (1981) Control of the cell cycle in cultured plant cells III. Effects of cytokinin. Protoplasma 106: 23–26.

    Article  CAS  Google Scholar 

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© 1993 Springer Science+Business Media Dordrecht

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Hanke, D.E. (1993). Cell cycle regulation in cultured cells. In: Ormrod, J.C., Francis, D. (eds) Molecular and Cell Biology of the Plant Cell Cycle. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-1789-0_13

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  • DOI: https://doi.org/10.1007/978-94-011-1789-0_13

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-4787-6

  • Online ISBN: 978-94-011-1789-0

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