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Polyamines and protein modification during the cell cycle

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Molecular and Cell Biology of the Plant Cell Cycle

Abstract

The behaviour of polyamines, proteins and other physiological parameters during the synchronous cell cycle following the break of dormancy of tubers of Helianthus tuberosus L. is presented. Polyamines seem to be relevant in this event. These growth substances may act in free or bound form; the covalently bound polyamines were analyzed both in vivo and in cell-free extracts which allows the determination of transglutaminase activity, the enzyme that links polyamines to proteins. This post-translational modification of proteins might have a structural or regulatory role. Free polyamine levels exhibit a bimodal fluctuation which peaks during the G1/S and the division phases of the cell cycle. This seems to be a common feature of plant and animal cycling cells. The level of conjugated polyamines, whose formation is catalyzed by a de novo synthesized transglutaminase, increases during the cell cycle showing a sigmoidal trend. The protein pattern on gel electrophoresis changes during the cell cycle showing an increase in the amount of high molecular mass bands.

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Authors
  1. S. Del Duca
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  2. D. Serafini-Fracassini
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Editor information

Editors and Affiliations

  1. I.C.I. Agrochemicals, Jealott’s Hill Research Station, Bracknell, UK

    J. C. Ormrod

  2. University of Wales, College of Cardiff, UK

    D. Francis

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© 1993 Springer Science+Business Media Dordrecht

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Del Duca, S., Serafini-Fracassini, D. (1993). Polyamines and protein modification during the cell cycle. In: Ormrod, J.C., Francis, D. (eds) Molecular and Cell Biology of the Plant Cell Cycle. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-1789-0_11

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  • DOI: https://doi.org/10.1007/978-94-011-1789-0_11

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-4787-6

  • Online ISBN: 978-94-011-1789-0

  • eBook Packages: Springer Book Archive

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