Abstract
A rapid and transient generation of activated oxygen from the elicitor-treated soybean cell suspension was detected with luminol-mediated chemiluminescense, and its generation was inhibited by addition of radical scavengers. Direct evidence for the existence of phosphatidylcholine hydroperoxide (PCOOH) and determination of the changes in PCOOH after treatment of soybean seedlings with fungal elicitor were examined using chemiluminescence-HPLC of hydroperoxide specific assay. PCOOH was transiently accumulated within 6 h after elicitor treatment preceding the induction of phytoalexin, but such event was not observed in control plants, thus suggesting that accumulation of PCOOH was a response to fungal elicitor in host plant. These rapid responses may be involved in the triggering of a plant defense mechanism against fungal invasion.
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© 1993 Springer Science+Business Media Dordrecht
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Kondo, Y., Hanawa, F., Miyazawa, T., Mizutani, J. (1993). Detection of Rapid and Transient Generation of Activated Oxygen and Phospholipid Hydroperoxide in Soybean after Treatment with Fungal Elicitor by Chemiluminescence Assay. In: Fritig, B., Legrand, M. (eds) Mechanisms of Plant Defense Responses. Developments in Plant Pathology, vol 2. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-1737-1_38
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DOI: https://doi.org/10.1007/978-94-011-1737-1_38
Publisher Name: Springer, Dordrecht
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