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Plant Breeding pp 126–137Cite as

Gene transfer to plants: approaches and available techniques

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Part of the book series: Plant Breeding Series ((PLBR))

Abstract

Gene transfer to plants is hampered by a variety of biological problems: the plant cell wall is a perfect barrier and trap for DNA molecules; egg cells, sperm cells and zygotes, are virtually inaccessible; proembryos are extremely small and enclosed within solid tissues; the tiny cells of hidden meristems which contribute to the ‘germline’ may not be competent for the integration of functional genes. There are no known retroviruses which could help systemically to spread and integrate foreign DNA. We have available only one functional biological vector system which, however, does not work with the agronomically most important groups or varieties of crop plants. Regeneration of transgenic plants mostly depends on ‘totipotent’ somatic cells, and, although some plant cells are totipotent, the majority, probably, are not.

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  1. I. Potrykus
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Editors and Affiliations

  1. Welsh Plant Breeding Station, AFRC IGER, Plas Gogerddan, Near Aberystwyth, SY23 3EB, Dyfed, UK

    M. D. Hayward

  2. Hilleshög AB, PO Box 302, S-26123, Landskrona, Sweden

    N. O. Bosemark

  3. Departamento de Producción Vegetal, UdL — IRTA, Avenida Rovira Roure 177, 25006, Lleida, Spain

    I. Romagosa

  4. CIHEAM, Zaragoza, Spain

    M. Cerezo

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Potrykus, I. (1993). Gene transfer to plants: approaches and available techniques. In: Hayward, M.D., Bosemark, N.O., Romagosa, I., Cerezo, M. (eds) Plant Breeding. Plant Breeding Series. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-1524-7_10

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  • DOI: https://doi.org/10.1007/978-94-011-1524-7_10

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-4665-7

  • Online ISBN: 978-94-011-1524-7

  • eBook Packages: Springer Book Archive

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