Abstract
Reconstitution of precursor transport using isolated intact chloroplasts was first demonstrated in the late 1970s using the precursor to the small subunit of ribulose bis-phosphate carboxylase [1,2]. Subsequent optimization and characterization has demonstrated that the in vitro reconstitution assay works with a number of precursor proteins [3] and faithfully mimics intra-organellar targeting as well as transport into the chloroplast [4, 5]. The in vitro reconstitution assays have been very useful in studying the details of the transport process as well as confirming the intracellular location of putative chloroplastic precursor proteins after the isolation of cDNA clones [6, 5]. Those readers desiring a detailed study of precursor protein transport into chloroplasts should consult the extensive literature on this topic [7–9]. This chapter is aimed at researchers with a cDNA clone encoding a putative precursor protein who wish to evaluate the transport competence of the precursor to confirm the intracellular location of the gene product.
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References
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© 1994 Springer Science+Business Media Dordrecht
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Bruce, B.D., Perry, S., Froehlich, J., Keegstra, K. (1994). In vitro import of proteins into chloroplasts. In: Gelvin, S.B., Schilperoort, R.A. (eds) Plant Molecular Biology Manual. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0511-8_32
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DOI: https://doi.org/10.1007/978-94-011-0511-8_32
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-011-7654-5
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