Abstract
Williams [14] and Welsh and McClelland [13] demonstrated the utility of single short oligonucleotide primers of arbitrary sequence for the amplification of DNA segments distributed randomly throughout the genome. Welsh and McClelland showed that the pattern of amplified bands could be used for genome fingerprinting [13] and Williams et al. [ 14] showed that the differences (polymorphisms) in the pattern of bands amplified from genetically distinct individuals behaved as mendelian genetic markers (named RAPDs, for Random Amplified Polymorphic DNA). Well-saturated maps of the Arabidopsis [11] and pine [4] genomes have been constructed using RAPD technology. A single set of arbitrary-sequence 10-mers may be used for mapping or fingerprinting any species. The many advantages of RAPD markers over RFLPs or isozyme markers accelerated the adoption of RAPD technology for the construction of genetic maps, fingerprinting, and population genetic studies [6]. Current reviews of the applications of RAPD technology are available [ 10, 12].
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References
Anon (1991) User Manual GenAmp PCR System 9600. Norwalk, CT: Perkin Elmer Cetus.
Arnold ML, Buckner CM, Robinson JJ (1991) Pollen-mediated introgression and hybrid speciation in louisiana irises. Proc Natl Acad Sci USA 88: 1398–1402.
Caetano-Anolles G, Bassam BJ, Gresshoff PM (1991) High resolution DNA amplification fingerprinting using very short arbitrary oligonucleotide primers. Biotechnology 9: 553–557.
Grattapaglia D, Chaparro J, Wilcox P, McCord S, Werner D, Amerson H, McKeand S, Bridgwater S, Whetten R, O’Malley D, Sederoff R (1992) Mapping in woody plants with RAPD markers: Application to breeding in forestry and horticulture. In: Proceedings of the Symposium ‘Application of RAPD Technology to Plant Breeding’, Joint Plant Breeding Symposia Series, Crop Science Society of America/American Society for Horticultural Science/American Genetic Association, November 1, 1992, Minneapolis, MN.
Dellaporta SL, Wood J, Hicks JB (1985) Maize DNA Miniprep. In: Molecular Biology of Plants, pp. 36–37. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
Hedrick P (1992) Shooting the RAPDs. Nature 355: 679–680.
Horn P, Rafalski, A (1992) Non-destructive RAPD Genetic diagnostics of microspore-derived Brassica embryos. Plant Mol Biol Rep 10: 281–289.
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular Cloning. A Laboratory Manual. (1st ed.). Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease resistance genes by bulked segregant analysis: A rapid method to direct markers in specific genomic regions using segregating populations. Proc Natl Acad Sci USA 88: 9828–9832.
Rafalski JAV, Tingey S, Williams JGK (1991) RAPD markers — a new technology for genetic mapping and plant breeding. AgBiotech News and Information 3: 645–648.
Reiter R, Williams JGK, Feldman KA, Rafalski JA, Tingey SV, Scolnik PA (1992) Global and local genome mapping in Arabidopsis thaliana using recombinant inbreds and random amplified polymorphic DNAs (RAPDs). Proc Natl Acad Sci USA 89: 1477–1481.
Tingey SV, del Tufo JP (1993) Genetic analysis with random amplified polymorphic DNA markers. Plant Physiol 101: 349–352.
Welsh J, McClelland M (1990) Fingerprinting genomes using PCR with arbitrary primers. Nucl Acid Res 19: 303–306.
Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl Acid Res 18: 6531–6535.
Williams JGK, Rafalski JA, Tingey SV (1993) Genetic analysis using RAPD markers. In: R. Wu (Eds.) Methods in Enzymology. Orlando, FL: Academic Press. Vol. 218, 704–740.
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© 1994 Springer Science+Business Media Dordrecht
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Rafalski, A., Tingey, S., Williams, J.G.K. (1994). Random amplified polymorphic DNA (RAPD) markers. In: Gelvin, S.B., Schilperoort, R.A. (eds) Plant Molecular Biology Manual. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0511-8_27
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DOI: https://doi.org/10.1007/978-94-011-0511-8_27
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