Abstract
Under iron-deficient conditions, strains of Rhizobium and Bradyrhizobium secrete iron-binding compounds (siderophore) to acquire iron. The objectives of the work were to evaluate the competitiveness for nodulation and the growth of a siderophore-producing Bradyrhizobium wild-type strain (sid+ parental strain) and those of its siderophore non-producing mutant (sid- mutant) in the rhizosphere of two provenances of Acacia mangium cultivated under iron-limited condition and also in the nonrhizosphere zone without any iron supplementation.
In the nonrhizosphere zone, population of the sid- mutant decreased markedly compared to that of the sid+ parental strain when the bacteria were alone. This suggests that the lack of iron in the milieu was more harmful to the sid- mutant than to the sid+ parental strain. However, in mixed inoculation experiments, the decrease of the sid- mutant’s population indicated above was significantly reduced suggesting that probably this strain might take benefits from the iron-binding siderophore presumably secreted by the parental strain.
In the rhizosphere, growth of both strains was apparently stimulated similarly by the root exudates from the two provenances of Acacia mangium studied here. Scrutiny of the R/S ratio however showed that at the early growth of the plants, provenances Iron Range and Dimisisi preferentially favoured growth of the parental strain and the mutant respectively. Consequently, it was found that when inoculating plants of the Iron Range provenance, the sid+ parental strain was more competitive than the sid- mutant as expressed by nodule occupancy. Inversely, the sid- mutant seemed to be more competitive than the sid+ parental strain when the provenance Dimisisi was used.
Our investigation therefore suggests that: 1) siderophore-producing ability may favour the persistence of Bradyrhizobium in iron-deficient soil; 2) the sid+ parental strain and its sid- mutant exhibited different growth responses and competitiveness according to the host-plant provenances whose role should not be neglected.
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References
Barran L R and Bromfield E S P 1993 Does siderophore production influence the relative abundance of Rhizobium meliloti in two field populations? Can. J. Microbiol. 39, 348–351.
Bremner J M and Mulvaney C S 1982 Nitrogen-total. In Methods of Soil Analysis. Ed. A L Page, part 2, pp 585–624. Am. Soc. Agron., Madison, USA.
Broughton W J and Dilworth M J 1971 Control of leghaemoglobin synthesis in snake beans. Biochem. J. 125, 1075–1080.
Carson K C, Holliday S, Glenn A R and Dilworth M J 1992 Siderophore and organic acid production in root nodule bacteria. Arch. Microbiol. 157, 264–271.
Compeau G, Jadoun Al-Achi B, Platsouka E and Levy S B 1988 Survival of rifampicin-resistant mutant of Pseudomonas fluorescens and Pseudomonas putida in soil systems. Appl. Environ. Microbiol. 54, 2432–2438.
Cooper J E 1978 A method for testing Rhizobium effectiveness at low pH. Soil Biol. Biochem. 10, 81–83.
Cooper J E 1979 Rapid method for counting antibiotic-resistant rhizobia in soils. Soil Biol. Biochem. 11, 433–435.
Crowley D E, Reid C P P and Szaniszlo P J 1987 Microbial siderophores as iron sources for plants. In Iron Transport in Microbes, Plants and Animals. Eds. G Winkelman, D Van der Helm and J B Neilands. pp 387–400. VCH editions, Weinhem, Germany.
Crowley D E, Wang Y C, Reid C P P and Szaniszlo P J 1991 Mechanisms of iron acquisition from siderophores by microorganisms and plants. Plant and Soil 130, 179–198.
Davies K G and Whitbread R 1989 In vitro studies of siderophore production by wild-type and rifampicin resistant strains of fluorescent Pseudomonads. Plant and Soil 116, 123–125.
De Weger L A, Schippers B and Lugtenberg B 1987 Plant growth stimulation by biological interference in iron metabolism in the rhizosphere. In Iron Transport in Microbes, Plants and Animals. Eds. G Winkelman, D Van der Helm and J B Neilands. pp 387–400. VCH editions, Weinhem, Germany.
Expert D and Gill P R 1991 Iron: a modulator in bacterial virulence and symbiotic nitrogen fixation. In Molecular Signals in Plant-Microbe Communications. Ed. D A S Verma. pp 229–245. CRC Press, Boca Raton.
Galiana A 1990 La symbiose fixatrice d’azote chez Acacia mangium-rhizobium. Thèse de Doctorat Université Paris VI, Paris.
Gill P R and Neilands J B 1989 Cloning a genomic region required for a high-affinity iron-uptake system in Rhizobium meliloti 1021. Mol. Microbiol. 3(9), 1183–1189.
Gill P R, Barton L L, Scoble M D and Neilands J B 1991 A high-affinity iron transport system of Rhizobium meliloti may be required for efficient nitrogen fixation in planta. Plant and Soil 130, 211–217.
Guerinot M L 1991 Iron uptake and metabolism in the rhizo-bia/legume symbioses. Plant and Soil 130, 199–209.
Hartmann A 1989 Ecophysiological aspects of growth and nitrogen fixation in Azospirillum spp. In Nitrogen Fixation with non-Legumes. Eds. F L Skinner, R M Boddey and I Fendrik. pp 123–136. Kluwer Academic Publishers, Dordrecht.
Hoben H J and Somasegaran P 1982 Comparison of the pour, spread and drop plate methods for enumeration of Rhizobium sp. in inoculants made from presterilised peat. Appl. Env. Microbiol. 44, 1246–1247.
Höfte M, Seong K Y, Jurkevitch E and Verstraete W 1991 Pyoverdin production by the plant growth beneficial Pseudomonas strain 7NSK2: Ecological significance in soil. Plant and Soil 130, 249–257.
Höfte M, Boelens J and Verstraete W 1992 Survival and root colonization of mutants of plant growth-promoting Pseudomonads affected in siderophore biosynthesis or regulation of siderophore production. J. Plant Nutr. 15, 2253–2262.
Jurkevitch E, Hadar Y and Chen Y 1988 Involvement of bacterial siderophores in the remedy of lime-induced chlorosis in peanut. Soil Sci. Soc. Am. J. 52, 1032–1037.
Jurkevitch E, Hadar Y and Chen Y 1992 Differential siderophore utilization and iron uptake by soil and rhizosphere bacteria. Appl. Env. Microbiol. 58, 119–124.
Lesueur D, Diem H G and Meyer J M 1993 Iron requirement and siderophore production in Bradyrhizobium strains isolated from Acacia mangium. J. Appl. Bacteriol. 74, 675–682.
Meyer J M and Abdallah M A 1978 The fluorescent pigment of Pseudomonas fluorescens: biosynthesis, purification and physiological properties. J. Gen. Microbiol. 107, 319–328.
Pankhurst C E 1977 Symbiotic effectiveness of antibiotic-resistant mutants of fast-and slow-growing strains of Rhizobium nodulating Lotus species. Can. J. Microbiol. 23, 1026–1033.
Plessner O, Klapatch T and Guerinot M L 1993 Siderophore utilization by Brady rhizobium japonicum. Appl. Env. Microbiol. 59, 1688–1690.
Rioux C R, Jordan D C and Rattray J B M 1986 Iron requirement of Rhizobium leguminosarum and secretion of anthranilic acid during growth on an iron-deficient medium. Arch. Biochem. Biophys. 248, 175–182.
Schwyn B and Neilands J B 1987 Universal chemical assay for the detection and determination of siderophores. Anal. Biochem. 160, 47–56.
Skorupska A, Derylo M and Lorkiewicz Z 1989 Siderophore production and utilization by Rhizobium trifolii. Biol. Metals 2, 45–49.
Smith M J and Neilands J B 1984 Rhizobactin, a siderophore from Rhizobium meliloti. J. Plant Nutr. 7, 449–458.
Tang C and Robson A D 1992 The role of iron in the (Brady)rhizobium legume symbiosis. J. Plant Nutr. 15(10), 2235–2252.
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Lesueur, D., del Carro Rio, M., Diem, H. (1995). Modification of the growth and the competitiveness of a Bradyrhizobium strain obtained through affecting its siderophore-producing ability. In: Abadía, J. (eds) Iron Nutrition in Soils and Plants. Developments in Plant and Soil Sciences, vol 59. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0503-3_9
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DOI: https://doi.org/10.1007/978-94-011-0503-3_9
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