Abstract
The effects of initial serum concentration, pH and stirrer speed on cell aggregation in CHO suspension cell culture were investigated. The results showed that cell aggregation was appreciably affected by the stage of the growth curve and cell density. The number of clumps increased during exponential and stationary phases. Increased serum concentration resulted in the formation of larger aggregate. However initial pH over a range of 6.8–7.6 showed no influence on either maxiumum cell number or cell aggregation. The effect of agitation on cell aggregation could not be distinguished from its effects on cell growth.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Boraston, R., Marshell, C., Norman, P., Renner, G. and Warner, J. (1992). Elimination of cell aggregation in suspension culture of chinese hamster ovary (CHO) cells. In: Animal cell technology: Developments, processes and products. (Ed. Spier, R.E., Griffiths, J.B. and Macdonald, C.), Butterworth-Heinemann, Oxford, p 424–426.
Coppen, S.R., Newsam, R., Baines, A.J. and Bull, A.T. (1994). Nature of cell-cell interactions between recombinant CHO cells expressing human interferon gamma. In: Animal cell technology: Products of today, prospects for tomorrow. ((Ed. Spier, R.E., Griffiths. J.B. and Berthold, W.). Butterworth-Heinemann. Oxford. p. 202–204.
Curtis, A.S.G. (1966). Cell adhesion. Sci. Prog., Oxf, 54 61–86.
Curtis, A.S.G. and Greaves, M.F. (1965). The inhibition of cell aggregation by a pure serum protein. J. Embryol. Exp. Morphol., 13 309–326.
Glasgow, L.A. and Kim, Y.M. (1986) Characterisation of agitation intensity in flocculation processes. J. Env. Eng. 112 1158–1163.
Gottesman, M.M. (1985). Molecular cell genetics. Wiley. New York, p 145.
Griffiths, B. (1986). Scaling-up of animal cell cultures. In: Animal cell culture: A practical approach. (Ed. Freshney. R.I.). IRL press, Oxford, p. 39.
Moreira, J.L., Alves, P.M., Aunins, J.G. and Carrondo, M.J.T. (1992). Aggregate suspension cultures of BHK cells. In: Animal cell technology: Developments. processes and products. (Ed. Spier. R.E., Griffiths. J.B. and Macdonald, C.), Butterworth-Heinemann, Oxford, 411–413.
Moreira, J.L., Feliciano, A.S., Alves, P.M.. Aunins. J.G. and Carrondo, M.J.T. (1994). Hydrodynamic control of suspended natural animal cell aggregates. In: Animal cell technology: Products of today, prospects for tomorrow. ((Ed. Spier, R.E., Griffiths, J.B. and Berthold, W.). Butterworth-Heinemann, Oxford, p. 397–401.
Renner, W.A., Jordan, M. and Eppenberger, H.M. (1993). Cell-Cell adhesion and aggregation: Influence on the growth behaviour of CHO cells. Biotechnol. Bioeng., 41 188–193.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1995 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Leelavatcharamas, V., Emery, A.N., Al-Rubeai, M. (1995). The Effects of Initial Serum Concentration, pH and Agitation Rate on Cell Aggregation During Batch Culture of Chinese Hamster Ovary (CHO) Cells. In: Beuvery, E.C., Griffiths, J.B., Zeijlemaker, W.P. (eds) Animal Cell Technology: Developments Towards the 21st Century. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0437-1_35
Download citation
DOI: https://doi.org/10.1007/978-94-011-0437-1_35
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-4195-9
Online ISBN: 978-94-011-0437-1
eBook Packages: Springer Book Archive