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Abstract

UK diagnostic laboratories use primary monkey kidney (PMK) cells as their main substrate for virus detection. In an attempt to obtain a substitute, thirty four immortalised Rhesus monkey kidney cells have been established by transfection with the SV40 large T antigen oncogene. Of these, five were found to approach primary Rhesus kidney cells in senstivity to human viruses and are being given further evaluation.

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References

  1. Clarke, J., MacDonald, C., Blake, K., and Griffiths, J.B. (1994) Immortalised cell lines for virus diagnosis. In Animal cell technology: Developments, processes, and products. (Eds. Spier, R.E., Griffiths, J.B., MacDonald, C.) Butterworth-Heinemann Ltd., Oxford, pp 661–663.

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  2. Clarke, J., MacDonald, C., Kreuzberg-Duffy, U., and Griffiths, J.B. (1994) Immortalised cell lines for virus diagnosis. In: Animal cell technology: products of today, prospects for tomorrow. (Eds. Spier, R.E., Griffiths, J.B., Berthold, W.). Butterworth-Heinemann Ltd., Oxford, pp 50–54.

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  3. Kreuzberg-Duffy, U., & MacDonald, C. (1994). Analysis of SV40 early region expression in immortalised mouse macrophage cell lines. In: Animal cell technology: products of today, prospects for tomorrow.(Eds. Spier, R.E., Griffiths, J.B., Berthold, W.)Butterworth-Heinemann Ltd., Oxford, pp 80–82.

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© 1995 Springer Science+Business Media Dordrecht

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Clarke, J.B., Kreuzberg-Duffy, U., MacDonald, C., Moulsdale, H., Golding, J., Griffiths, B. (1995). Human Virus Detection Using Cells Immortalised by Oncogenes. In: Beuvery, E.C., Griffiths, J.B., Zeijlemaker, W.P. (eds) Animal Cell Technology: Developments Towards the 21st Century. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0437-1_1

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  • DOI: https://doi.org/10.1007/978-94-011-0437-1_1

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-4195-9

  • Online ISBN: 978-94-011-0437-1

  • eBook Packages: Springer Book Archive

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