Summary
PEG fusion solution for leaf protoplasts of Petunia parodii and cell suspension protoplasts of albino P. hybrida cv. Comanche was supplemented with 0.01–1.0% (w/v) Pluronic F-68. This stimulated protoplast fusion overall, including parental homokaryon formation, with increased means of 23% and 83% respectively, over appropriate controls using 1.0% (w/v) surfactant added to the standard PEG solution. Interestingly, the percentage heterokaryon formation increased near 2-fold (P < 0.001) for fusogen solutions supplemented with 0.01% (w/v) Pluronic. Protoplasts regenerated to colonies in KM8P/KM8 liquid medium, indicating no adverse effects of Pluronic F-68 on viability, both in the short and longer terms.
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Abbreviations
- BA:
-
6-benzyladenine
- MS:
-
Murashige & Skoog (1962)
- NAA:
-
α-naphthaleneacetic acid
- PEG:
-
polyethylene glycol
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Hill, T.K., Davey, M.R., Power, J.B., Anthony, P., Lowe, K.C. (1995). Improved plant heterokaryon formation by surfactant-supplementation of polyethylene glycol fusogen solution. In: Cassells, A.C., Jones, P.W. (eds) The Methodology of Plant Genetic Manipulation: Criteria for Decision Making. Developments in Plant Breeding, vol 3. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0357-2_31
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