Summary
Leaf explants of apple cvs Gala and Golden Delicious were infected with the Agrobacterium tumefaciens strain AGL0(pMOG410). The effects of a 2 d preculture of the explants before infection and the addition of 5-azacytidine to the selection medium were studied. The percentages of GUS-positive explants after 5 w did not significantly alter due to these treatments. One of the ‘Gala’ shoots, which was removed from a leaf explant cultured for 8 w on selection medium, proved to be GUS-positive and will be analyzed further. In general, however, it should be concluded that regeneration of transgenic shoots directly from leaf tissue was not very effective.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Abbreviations
- MS:
-
Murashige and Skoog medium
- GD:
-
‘Golden Delicious’
References
Goodwin, P.B., S. Sriskandarajah & J. Speirs, 1993. Genetic transformation of apple. In: T. Hayashi et al. (Eds). Techniques on Gene Diagnosis and Breeding in Fruit Trees, pp. 178–183. FTRS, Japan.
James, DJ., A.J. Passey, D.J. Barbara & M. Bevan, 1989. Genetic transformation of apple (Malus pumila Mill.) using a disarmed Ti-binary vector. Plant Cell Rep. 7: 658–661.
Jefferson, R.A., T.A. Kavanagh & M.W. Bevan, 1987. GUS fusions: ?-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 6: 3901–3907.
Mandal, A., V. Lang, W. Orczyk & E.T. Palva, 1993. Improvedefficiency for T-DNA-mediated transformation and plasmid rescue in Arabidopsis thaliana. Theor. Appl. Genet. 86: 621–628.
McHughen, A., M. Jordan & G. Feist, 1989. A preculture period prior to Agrobacterium inoculation increases production of transgenic plants. J. Plant Physiol. 135: 245–248.
Maheswaran, G., M. Welander, J.F. Hutchinson, M.W. Graham & D. Richards, 1992. Transformation of apple rootstock M26 with Agrobacterium tumefaciens. J. Plant Physiol. 139: 560–568.
Sangwan, R.S., Y. Bourgeois, S. Brown, G. Vasseur & B. Sangwan- Norreel, 1992. Characterization of competent cells and early events of Agrobacterium-mediatedgenetic transformation in Arabidopsis thaliana. Planta 188: 439–456.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1995 Springer Science+Business Media New York
About this chapter
Cite this chapter
Schaart, J.G., Puite, K.J., Kolova, L., Pogrebnyak, N. (1995). Some methodological aspects of apple transformation by Agrobacterium . In: Cassells, A.C., Jones, P.W. (eds) The Methodology of Plant Genetic Manipulation: Criteria for Decision Making. Developments in Plant Breeding, vol 3. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0357-2_17
Download citation
DOI: https://doi.org/10.1007/978-94-011-0357-2_17
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-4159-1
Online ISBN: 978-94-011-0357-2
eBook Packages: Springer Book Archive