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Molecular Microbial Ecology Manual pp 89–108Cite as

Extraction and PCR amplification of DNA from the rhizoplane

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Abstract

While the term rhizoplane has been adopted for the plant root surface [8], the root environment represents a plant-soil-microbial continuum [40]. That is, microbial communities are found to transect the root, from the soil under the influence of the root (rhizosphere) through to the cortical tissue. The root surface is a rapidly changing substrate, particularly during the first 10 days following emergence of the radicle from the seed [13]. Root habitats are characterized by environmental gradients, including reduced oxygen tensions, elevated CO2 levels as a result of plant and microbial respiration, pH differences and negative water potentials caused by evaporation at the leaf surface and subsequent transpiration.

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Author information

Authors and Affiliations

  1. Molecular Microbial Ecology, Institute of Virology and Environmental Microbiology, Natural Environment Research Council, Mansfield Road, Oxford, OX1 3SR, UK

    Penny A. Bramwell & Mark J. Bailey

  2. Analytical Molecular Biology Group, Laboratory of the Government Chemist, Queens Road, Teddington, Middlesex, TW11 OLY, UK

    Rita V. Barallon & Hilary J. Rogers

Authors
  1. Penny A. Bramwell
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  2. Rita V. Barallon
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  3. Hilary J. Rogers
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  4. Mark J. Bailey
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Editor information

Editors and Affiliations

  1. Department of Microbiology, Wageningen Agricultural University, The Netherlands

    Antoon D. L. Akkermans

  2. IPO-DLO, Wageningen, The Netherlands

    Jan Dirk Van Elsas

  3. MSU-DOE Plant Research Lab, NSF Center for Microbial Ecology and Department of Microbiology, Michigan State University, USA

    Frans J. De Bruijn

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© 1995 Springer Science+Business Media Dordrecht

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Bramwell, P.A., Barallon, R.V., Rogers, H.J., Bailey, M.J. (1995). Extraction and PCR amplification of DNA from the rhizoplane. In: Akkermans, A.D.L., Van Elsas, J.D., De Bruijn, F.J. (eds) Molecular Microbial Ecology Manual. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0351-0_9

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  • DOI: https://doi.org/10.1007/978-94-011-0351-0_9

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-4156-0

  • Online ISBN: 978-94-011-0351-0

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