Abstract
The sensitivity of random amplified polymorphic DNA using arbitrary 10-mer oligonucleotide primers (RAPD) to detect genetic change in sugarcane (Saccharum spp. hybrid) plants during tissue culture was assessed. RAPD analysis of sugarcane plants regenerated from embryogenic callus revealed very few polymorphisms. However, RAPDs detected gross genetic change in protoplast-derived callus of sugarcane. This callus had been in tissue culture for over two years and had lost the ability to differentiate shoots and regenerate plants. RAPD assessment of the genetic integrity of sugarcane plants from an in vitro germplasm collection, regenerated from apical buds and maintained in storage at 18°C for 3, 6 and 12 months, revealed no significant genetic change. These results suggest that RAPDs are suitable for detecting gross genetic change but may not be sensitive enough to detect small changes and that minimal genetic change appears to take place in sugarcane plants regenerated from embryogenic callus or apical buds.
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© 1995 Springer Science+Business Media Dordrecht
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Taylor, P.W.J., Fraser, T.A., Ko, HL., Henry, R.J. (1995). RAPD Analysis of Sugarcane During Tissue Culture. In: Terzi, M., Cella, R., Falavigna, A. (eds) Current Issues in Plant Molecular and Cellular Biology. Current Plant Science and Biotechnology in Agriculture, vol 22. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0307-7_32
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DOI: https://doi.org/10.1007/978-94-011-0307-7_32
Publisher Name: Springer, Dordrecht
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