Abstract
The relationship between growth and interferon-γ (IFN-γ) production in the recombinant cell line CHO 320 was studied by varying the foetal calf serum (FCS) concentration. The specific growth rate varied with the initial FCS concentration in a manner which could be well fitted by the Monod model. The K s and μ max values were found to be 0.771% (v/v) serum and 0.031 h-1 respectively. The average specific IFN-γ production rates during the exponential phase increased with increasing FCS concentration. A good correlation between specific production rate and specific growth rate was found in all phases of the culture except the lag phase and it was clearly demonstrated that IFN-γ production was growth associated. Specific glucose and glutamine utilisation rates were inversely related to specific growth rates.
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© 1994 Springer Science+Business Media Dordrecht
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Leelavatcharamas, V., Emery, A.N., Al-Rubeai, M. (1994). Growth and interferon-γ production in batch culture of CHO cells. In: Buckland, B.C., Aunins, J.G., Bibila, T.A., Hu, WS., Robinson, D.K., Zhou, W. (eds) Cell Culture Engineering IV. Current Applications of Cell Culture Engineering, vol 1. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0257-5_8
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DOI: https://doi.org/10.1007/978-94-011-0257-5_8
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