Abstract
In a batch-refeed continuous process involving a recombinant Chinese hamster ovary cell line, a brief upset was occasionally observed during which cell growth halted and cell viability dropped. This was found to be associated with depletion of insulin from the medium early during the affected passage. Insulin depletion was found to be primarily the result of insulin degrading activity released by the cells during the preceding passage.
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Abbreviations
- CHO:
-
Chinese hamster ovary
- rhM-CSF:
-
recombinant human macrophage colony stimulating factor
- RIA:
-
radioimmunoassay
- RP-HPLC:
-
reverse phase high performance liquid chromatography
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© 1994 Springer Science+Business Media Dordrecht
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Drapeau, D. et al. (1994). Extracellular insulin degrading activity creates instability in a CHO-based batch-refeed continuous process. In: Buckland, B.C., Aunins, J.G., Bibila, T.A., Hu, WS., Robinson, D.K., Zhou, W. (eds) Cell Culture Engineering IV. Current Applications of Cell Culture Engineering, vol 1. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0257-5_12
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DOI: https://doi.org/10.1007/978-94-011-0257-5_12
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-4114-0
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