Molecular genetics of auxin and cytokinin

  • Lawrence Hobbie
  • Candace Timpte
  • Mark Estelle


The indeterminate nature of plant development requires that plants continuously regulate cell division and elongation in response to genetic, as well as a wide variety of different environmental signals [53]. Since their discovery, many physiological studies have shown that the plant hormones auxin and cytokinin have fundamental roles in the regulation of cell growth [30, 43]. Both compounds appear to be essential for growth of plant cells in culture. Organogenesis in tissue culture is regulated by altering the concentration of auxin and cytokinin. In the intact plant, auxin and cytokinin are apparently involved in a bewildering array of growth processes. One explanation for this complexity is that most and perhaps all changes in cell division or elongation are mediated by cytokinin or auxin. According to this view, developmental responses to environmental stimuli such as light or temperature are mediated by these hormones.

Key words

auxin cytokinin plant hormones plant development 


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  1. 1.
    Abel S, Oeller PW, Theologis A: Early auxin-induced genes encode short-lived nuclear proteins. Proc Natl Acad Sci USA 91: 326–330 (1994).PubMedCrossRefGoogle Scholar
  2. 2.
    Abel WO, Knebel W, Koop HU, Marienfeld JR, Quader H, Reski R, Schnepf E, Spoerlein B: A cytokinin rensitive mutant of the moss Physcomitrella patens, defective in chloroplast division. Protoplasma 152: 1–13 (1989).CrossRefGoogle Scholar
  3. 3.
    Ainley WM, Walker JC, Nagao RT, Key JL: Sequence and characterization of two auxin-regulated genes from soybean. J Biol Chem 263: 10658–10666 (1988).PubMedGoogle Scholar
  4. 4.
    Ainley WM, McNeil KJ, Hill JW, Lingle WL, Simpson RB, Brenner ML, Nagao RT, Key JL: Regulatable endogenous production of cytokinins up to ‘toxic’ levels in transgenic plants and plant tissues. Plant Mol Biol 22: 13–23 (1993).PubMedCrossRefGoogle Scholar
  5. 5.
    Akiyoshi D, Klee H, Amasino R, Nester EW, Gordon MP: T-DNA of Agrobacterium tumefaciens encodes an enzyme of cytokinin biosynthesis. Proc Natl Acad Sci USA 81: 5994–5998 (1984).PubMedCrossRefGoogle Scholar
  6. 6.
    Alliotte T, Tire C, Engler G, Peleman J, Caplan A, Van Montagu M, Inze D: An auxin-regulated gene of Arabidopsis thaliana encodes a DNA-binding protein. Plant Physiol 89: 743–752 (1989).PubMedCrossRefGoogle Scholar
  7. 7.
    Anderson JA, Huprikar SS, Kochian LV, Lucas WJ, Gaber RF: Functional expression of a probable Arabidopsis thaliana potassium channel in Saccharomyces cerevisiae. Proc Natl Acad Sci USA 89: 3736–3740 (1992).PubMedCrossRefGoogle Scholar
  8. 8.
    Andre B, Scherer GFE: Stimulation by auxin of phospholipase A in membrane vesicles from an auxinsensitive tissue is mediated by an auxin receptor. Planta 185: 209–214 (1991).CrossRefGoogle Scholar
  9. 9.
    Ayling SM, Brownlee C, Clarkson DT: The cytoplasmic streaming response of tomato root hairs to auxin; observations of cytosolic calcium levels. J Plant Physiol 143: 184–188 (1994).CrossRefGoogle Scholar
  10. 10.
    Ballas N, Wong L-M, Theologis A: Identification of the auxin-responsive element, AuxRE, in the primary indoleacetic acid-inducible gene, PS-1AA4/5, of pea (Pisum sativum L.). J Mol Biol 233: 580–596 (1993).PubMedCrossRefGoogle Scholar
  11. 11.
    Barbier-Brygoo H, Ephritikhine G, Klambt D, Ghislain M, Guem J: Functional evidence for an auxin receptor at the plasmalemma of tobacco mesophyll protoplasts. Proc Natl Acad Sci USA 86: 891–895 (1989).PubMedCrossRefGoogle Scholar
  12. 12.
    Barbier-Brygoo H, Ephritikhine G, Klambt D, Maurel C, Palme K, Schell J, Guern J: Perception of the auxin signal at the plasma membrane of tobacco mesophyll protoplasts. Plant J 1: 83–93 (1991).CrossRefGoogle Scholar
  13. 13.
    Bates GW. Goldsmith MHM: Rapid response of the plasma-membrane potential in oat coleoptiles to auxin and other weak acids. Planta 159: 231–237 (1983).CrossRefGoogle Scholar
  14. 14.
    Bilang J, Macdonald H, King PJ, Sturm A: A soluble auxin-binding protein from Hyoscyamus muticus is a glutathione S-transferase. Plant Physiol 102: 29–34 (1993).PubMedCrossRefGoogle Scholar
  15. 15.
    Binns AN, Labliola J, Black RC: Initiation of auxin autonomy in Nicotiana glutinosa cells by the cytokinin-biosynthesis gene from Agrobacterium tumefaciens. Planta 171: 539–548 (1987).CrossRefGoogle Scholar
  16. 16.
    Blatt MR, Thiel G: K + channels of stomatal guard cells: bimodal control of the K+ inward-rectifier evoked by auxin. Plant J 5: 55–68 (1994).PubMedCrossRefGoogle Scholar
  17. 17.
    Blonstein AD, Vahala T, Koornneef M, King PJ: Plants regenerated from auxin-auxotrophic variants are inviable. Mol Gen Genet 215: 58–64 (1988).CrossRefGoogle Scholar
  18. 18.
    Blonstein AD, Parry AD, Horgan R, King PJ: A cytokinin-resistant mutant of Nicotiana plumbaginifolia is wilty. Planta 183: 244–250 (1991).CrossRefGoogle Scholar
  19. 19.
    Boerjan W, Den Boer B, Van Montagu M: Molecular genetic approaches to plant development. Int J Devel Biol 36: 59–66 (1992).Google Scholar
  20. 20.
    Brock TG, Burg J, Ghosheh NS, Kaufman PB: The role of calcium in growth induced by indole-3-acetic acid and gravity in the leaf-sheath pulvinus of oat (Avena sativa). J Plant Growth Regul 11: 99–103 (1992).PubMedCrossRefGoogle Scholar
  21. 21.
    Brummell DA, Hall JL: Rapid cellular responses to auxin and the regulation of growth. Plant Cell Environ 10: 523–543 (1987).Google Scholar
  22. 22.
    Bush DS: Regulation of cytosolic calcium in plants. Plant Physiol 103: 7–13 (1993).PubMedGoogle Scholar
  23. 23.
    Campos N, Bako L, Feldwisch J, Schell J, Palme K: A protein from maize labeled with azido-IAA has novel beta-glucosidase activity. Plant J 2: 675–684 (1992).CrossRefGoogle Scholar
  24. 24.
    Chaudhury AM, Letham S, Craig S, Dennis ES: AMPl-a mutant with high cytokinin levels and altered embryonic pattern, faster vegetative growth, constitutive photomorphogenesis and precocious flowering. Plant J 4: 907–916(1993).CrossRefGoogle Scholar
  25. 25.
    Chen C-M, Jin G, Anderson BR, Ertl J: Modulation of plant gene expression by cytokinins. Aust J Plant Physiol 20: 609–619 (1993).CrossRefGoogle Scholar
  26. 26.
    Chory J, Reinecke D, Sim S, Washburn T, Brenner M: A role for cytokinins in de-etiolation in Arabidopsis: Plant Physiol 104: 339–347 (1994).PubMedGoogle Scholar
  27. 27.
    Conner TW, Goekjian VH, LaFayette PR, Key JL: Structure and expression of two auxin-inducible genes from Arabidopsis. Plant Mol Biol 15: 623–632 (1990).PubMedCrossRefGoogle Scholar
  28. 28.
    Cross JW: Cycling of auxin-binding protein through the plant cell: pathways in auxin signal transduction. New Biol 3: 813–819 (1991).PubMedGoogle Scholar
  29. 29.
    Datta N, LaFayette PR, Kroller PA, Nagao RT, Key JL: Isolation and characterization of three families of auxin down-regulated cDNA-clones. Plant Mol Biol 21: 859–869 (1993).PubMedCrossRefGoogle Scholar
  30. 30.
    Davies PJ: The plant hormones: Their nature, occurrence, and functions. In: Davies PJ (ed) Plant Hormones and their Role in Plant Growth and Development, pp. 1–11. Kluwer Academic Publishers, Dordrecht (1988).Google Scholar
  31. 31.
    DeWald DB, Sadka A, Mullet JE: Sucrose modulation of soybean Vsp gene expression is inhibited by auxin. Plant Physiol 104: 439–444 (1994).PubMedGoogle Scholar
  32. 32.
    Dominov JA, Stenzler L, Lee S, Schwarz JJ, Leisner S, Howell SH: Cytokinins and auxins control the expression of a gene in Nicotiana plumbaginifolia cells by feedback regulation. Plant Cell 4: 451–461 (1992).PubMedGoogle Scholar
  33. 33.
    Droog FNJ, Hooykaas PJJ, Libbenga KR, van der Zaal EJ: Proteins encoded by an auxin-regulated gene family of tobacco share limited but significant homology with glutathiones-transferases and one member indeed shows in vitro GST activity. Plant Mol Biol 21: 965–972 (1993).PubMedCrossRefGoogle Scholar
  34. 34.
    Earnshaw BA, Johnson MA: The effect of glutathione on development in wild carrot suspension cultures. Biochem Biophys Res Comm 133: 988–993 (1985).PubMedCrossRefGoogle Scholar
  35. 35.
    Einspahr KJ, Thompson GA Jr: Transmembrane signalling via phosphatidylinositol 4,5-bisphosphate hydrolysis in plants. Plant Physiol 93: 361–355 (1990).PubMedCrossRefGoogle Scholar
  36. 36.
    Ephritikhine G, Barbier-Brygoo H, Muller J-F, Guern J: Auxin effects on the transmembrane potential difference of wild-type and mutant tobacco protoplasts exhibiting a differential sensitivity to auxin. Plant Physiol 83: 801–804 (1987).PubMedCrossRefGoogle Scholar
  37. 37.
    Esaka M: Regulation of ascorbate oxidase gene expression in higher plants. Vitamins 67: 301–310 (1993).Google Scholar
  38. 38.
    Estelle M, Klee HJ: Auxin and cytokinin in Arabidopsis. In: Meyerowitz E, Somerville C, (eds), Cold Spring Harbor Press, Cold Spring Harbor, NY, (1994).Google Scholar
  39. 39.
    Estruch J J, Granell A, Hansen G, Prinsen E, Redig P, Vanonckelen H, Schwarzsommer Z, Sommer H, Spena A: Floral development and expression of floral homeotic genes are influenced by cytokinins. Plant J 4: 379–384 (1993).PubMedCrossRefGoogle Scholar
  40. 40.
    Estruch JJ, Parets-Soler A, Schmulling T, Spena A: Cytosolic localization in transgenic plants of the rolC peptide from Agrobacterium rhizogenes. Plant Mol Biol 17: 547–550 (1991).PubMedCrossRefGoogle Scholar
  41. 41.
    Estruch J J, Prinsen E, VanOnckelen H, Schell J, Spena A: Viviparous leaves produced by somatic activation of an inactive cytokinin-synthesizing gene. Science 254: 1364–1367 (1991).PubMedCrossRefGoogle Scholar
  42. 42.
    Ettlinger C, Lehle L: Auxin induces rapid changes in phosphatidylinositol metabolites. Nature 331: 176–178 (1988).PubMedCrossRefGoogle Scholar
  43. 43.
    Evans ML: Functions of hormones at the cellular level of organization. In: TK Scott (ed), Hormonal Regulation of Development II. Encyclopedia of Plant Physiology, vol, 10, pp. 23–79. Springer-Verlag, Berlin (1984).CrossRefGoogle Scholar
  44. 44.
    Featherstone DR, Cove DJ, Ashton NW: Genetic analysis by somatic hybridization of cytokinin overproducing developmental mutants of the moss Physcomitrella patens. Mol Gen Genet 222: 217–224 (1990).PubMedCrossRefGoogle Scholar
  45. 45.
    Feldwisch J, Zette R, Hesse F, Schell J, Palme K: An auxin-binding protein is localized to the plasma membrane of maize coleoptile cells: Identification by photo-affinity labeling and purification of a 23-kDa polypeptide. Proc Natl Acad Sci USA 89: 475–479 (1992).PubMedCrossRefGoogle Scholar
  46. 46.
    Felle H: Auxin causes oscillations of cytosolic free calcium and pH in Zea mays coleoptiles. Planta 174: 495–499 (1988).CrossRefGoogle Scholar
  47. 47.
    Felle H, Peters W, Palme K: The electrical response of maize to auxins. Biochim Biophys Acta 1064: 199–204 (1991).PubMedCrossRefGoogle Scholar
  48. 48.
    Fracheboud Y, King PJ: An auxin-auxotrophic mutant of Nicotiana plumbaginifolia. Mol Gen Genet 227: 397–400 (1991).PubMedCrossRefGoogle Scholar
  49. 49.
    Franco AR, Gee MA, Guilfoyle TJ: Induction and superinduction of auxin-responsive mRNAs with auxin and protein synthesis inhibitors. J Biol Chem 265: 15845–15849 (1990).PubMedGoogle Scholar
  50. 50.
    Gabathuler R, Cleland RE: Auxin regulation of a proton translocating ATPase in pea root plasma membrane vesicles. Plant Physiol 79: 1080–1085 (1985).PubMedCrossRefGoogle Scholar
  51. 51.
    Gehling CA, Irving HR, Parish RW: Effects of auxin and abscisic acid on cytosolic calcium and pH in plant cells. Proc Natl Acad Sci USA 87: 9645–9649 (1990).CrossRefGoogle Scholar
  52. 52.
    Gil P, Liu Y, Orbovic V, Verkamp E, Poff KL, Green PJ: Characterization of the auxin-inducible SAUR-AC1 gene for use as a molecular genetic tool in Arabidopsis. Plant Physiol 104: 777–784 (1994).PubMedCrossRefGoogle Scholar
  53. 53.
    Goldberg, R: Plants: Novel developmental processes. Science 240: 1460–1467 (1988).PubMedCrossRefGoogle Scholar
  54. 54.
    Goldsmith MHM: Cellular signalling: new insights into the action of the plant growth hormone auxin. Proc Natl Acad Sci USA 90: 11442–11445 (1993).PubMedCrossRefGoogle Scholar
  55. 55.
    Gonzalez-Daros F, Carrasco-Luna J, Calatayud A, Salguero J, del Valle-Tascon S: Effects of calmodulin antagonists on auxin-stimulated proton extrusion in Avena sativa coleoptile segments. Physiol Plant 87: 68–76 (1993).CrossRefGoogle Scholar
  56. 56.
    Guilfoyle TJ, Hagen G, Li Y, Ulmasov T, Liu Z, Strabala T, Gee M: Auxin-regulated transcription. Aust J Pant Physiol 20: 489–502 (1993).CrossRefGoogle Scholar
  57. 57.
    Hagen G, Kleinschmidt A, Guilfoyle T: Auxin-regulated gene expression in intact soybean hypocotyl and excised hypocotyl sections. Planta 162: 147–153 (1984).CrossRefGoogle Scholar
  58. 58.
    Hager A, Debus G, Edel HG, Stransky H, Serrano R: Auxin induces exocytosis and the rapid synthesis of a high-turnover pool of plasma-membrane H + -ATPase. Planta 185: 527–537 (1991).CrossRefGoogle Scholar
  59. 59.
    Hayashi H, Czaja I, Lubenow H, Schell J, Walden R: Activation of a plant gene by T-DNA tagging: auxin-independent growth in vitro. Science 258: 1350–1353 (1992).PubMedCrossRefGoogle Scholar
  60. 60.
    Hedrich R, Marten I: Malate-induced feedback regulation of plasma membrane anion channels could provide a CO2 sensor to guard cells. EMBO J 12: 897–901 (1993).PubMedGoogle Scholar
  61. 61.
    Hemerly AS, Ferreira P. Engler JdeA, Van Montagu M, Engler G, Inze D: cdc2a expression in Arabidopsis is linked with competence for cell division. Plant Cell 5: 1711–1723(1993).PubMedGoogle Scholar
  62. 62.
    Hershko A, Ciechanover A: The ubiquitin system for protein degradation. Annu Rev Biochem 61: 69–102 (1992).CrossRefGoogle Scholar
  63. 63.
    Hesse T, Feldwisch J, Balshusemann D, Bauw G, Puype M, Vandekerckhove J, Lobler M, Klambt D, Schell J, Palme K: Molecular cloning and structural analysis of a gene from Zea mays (L.) coding for a putative receptor for the plant hormone auxin. EMBO J 8: 2453–2461 (1989).PubMedGoogle Scholar
  64. 64.
    Hicks GR, Rice MS, Lomax TL: Characterization of auxin-binding proteins from zucchini plasma membrane. Planta 189: 83–90 (1993).PubMedCrossRefGoogle Scholar
  65. 65.
    Hirasawa E, Yamamoto S: Properties and synthesis de novo of auxin-induced alpha-amylase in pea cotyledons. Planta 184: 438–442 (1991).CrossRefGoogle Scholar
  66. 66.
    Ho T-hD, Hagen G: Hormonal regulation of gene expression. J Plant Growth Regul 12: 197–205 (1993).CrossRefGoogle Scholar
  67. 67.
    Hobbie L, Estelle M: Genetic approaches to auxin action. Plant Cell Environ 17: 525–540 (1994).PubMedCrossRefGoogle Scholar
  68. 68.
    Inhohara N, Shimomura S, Fukui T, Futai M: Auxinbinding protein located in the endoplasmic reticulum of maize shoots: Molecular cloning and complete primary structure. Proc Natl Acad Sci USA 86: 3564–3568 (1989).CrossRefGoogle Scholar
  69. 69.
    Irving HR, Gehring CA, Parish RW: Changes in cytosolic pH and calcium of guard cells precede stomatal movements. Proc Natl Acad Sci USA 89: 1790–1794 (1992).PubMedCrossRefGoogle Scholar
  70. 70.
    John PCL. Zhang K, Dong C, Diedelich L, Wightman F: p34cdc2 related proteins in control of cell cycle progression, the switch between division and differen-tiation in tissue development, and stimulation of division auxin and cytokinin. Aust J Plant Physiol 20: 503–526 (1993).CrossRefGoogle Scholar
  71. 71.
    Jonak C, Heberle-Bors E, Hirt H: MAP kinases: universal multi-purpose signalling tools. Plant Mol Biol 24: 407–416 (1994).PubMedCrossRefGoogle Scholar
  72. 72.
    Jones AM: Do we have the auxin receptor yet? Physiol Plant 80: 154–158 (1990).CrossRefGoogle Scholar
  73. 73.
    Jones AM, Herman EM: KDEL-containing auxin-binding protein is secreted to the plasma membrane and cell wall. Plant Physiol 101: 595–606 (1993).PubMedGoogle Scholar
  74. 74.
    Kaminek M: Progress in cytokinin research. Trends Biotechnol 10: 159–164 (1992).CrossRefGoogle Scholar
  75. 75.
    Kernan A, Thornburg RW: Auxin levels regulate the expression of a wound-inducible proteinase inhibitor II-chloramphenicol acetyl transferase gene fusion in vitro and in vivo. Plant Physiol 91: 73–78 (1989).PubMedCrossRefGoogle Scholar
  76. 76.
    Klee HJ, Horsch RB, Hinchee MA, Hein MB, Hoffmann NL: The overproduction of two Agrobacterium tumefaciens T-DNA auxin biosynthetic gene products in transgenic petunia plants. Genes Devel 1: 86–96 (1987).CrossRefGoogle Scholar
  77. 77.
    Klee H, Estelle M: Molecular genetic approaches to plant hormone biology. Annu Rev Plant Physiol Plant Mol Biol 42: 529–551(1991).CrossRefGoogle Scholar
  78. 78.
    Knight MR. Read NC, Campbell AK, Trewavas AJ: Imaging calcium dynamics in living plants using semisynthetic recombinant aequorins. J Cell Biol 121: 83–90 (1993).PubMedCrossRefGoogle Scholar
  79. 79.
    Kutschera U, Schopfer P: Evidence against the acid-growth theory of auxin action. Planta 163: 483–493 (1985).CrossRefGoogle Scholar
  80. 80.
    Lazarus CM, Napier RM, Yu L-X, Lynas C, Venis MA: Auxin-binding proteins-antibodies and genes. In: Jenkins GI, Schuch W (eds) Molecular Biology of Plant Development, pp. 129–148, Company of Biologists Ltd, Cambridge, UK, (1991).Google Scholar
  81. 81.
    Letham DS. Palni LMS: The biosynthesis and metabolism of cytokinins. Ann Rev Plant Phys 34: 163–197 (1983).CrossRefGoogle Scholar
  82. 82.
    Leyser HMO, Lincoln CA, Timpte C, Lammer D, Turner J, Estelle M: Arabidopsis auxin-resistance gene AXR1 encodes a protein related to ubiquitin-activating enzyme El. Nature 364: 161–164 (1993).PubMedCrossRefGoogle Scholar
  83. 83.
    Li Y, Hagen G, Guilfoyle TJ: An auxin-responsive promoter is differentially induced by auxin gradients during tropisms. Plant Cell 3: 1167–1175 (1991).PubMedGoogle Scholar
  84. 84.
    Li Y, Hagen G, Guilfoyle TJ: Altered morphology in transgenic tobacco plants that overproduce cytokinins in specific tissues and organs. Devel Biol 153: 386–395 (1992).CrossRefGoogle Scholar
  85. 85.
    Lincoln C, Britton JH, Estelle M: Growth and development of the axrl mutants of Arabidopsis. Plant Cell 2: 1071–1080 (1990).PubMedGoogle Scholar
  86. 86.
    Löbler M, Klämbt D: Auxin-binding protein from coleoptile membranes of com (Zea mays L.), I. Purification by immunological methods and characterization. J Biol Chem 260: 9848–9853 (1985).PubMedGoogle Scholar
  87. 87.
    Löbler M, Klämbt D: Auxin-binding protein from coleoptile membranes of com (Zea mays L.). II. Localization of a putative auxin receptor. J Biol Chem 260: 9854–9859 (1985).PubMedGoogle Scholar
  88. 88.
    Lohse G, Hedrich R: Characterization of the plasma membrane H + -ATPase from Vicia faba guard cells. Modulation by extracellular factors and seasonal changes. Planta 188: 206–214 (1992).CrossRefGoogle Scholar
  89. 89.
    MacDonald H, Jones AM, King PJ: Photoaffinity labeling of soluble auxin-binding proteins. J Biol Chem 266: 7393–7399 (1991).PubMedGoogle Scholar
  90. 90.
    Marten I, Lohse G, Hedrich R: Plant growth hormones control voltage-dependent activity of anion channels in plasma membrane of guard cells. Nature 353: 758–762 (1991).CrossRefGoogle Scholar
  91. 91.
    Marten I, Zeilinger C, Redhead C, Landry DW, Al-Awqati Q, Hedrich R: Identification and modulation of a voltage-dependent anion channel in the plasma membrane of guard cells by high-affinity ligands. EMBO J 11: 3569–3575 (1992).PubMedGoogle Scholar
  92. 92.
    Martinez MC, Jørgensen J-E, Lawton MA, Lamb CJ, Doerner PW: Spatial patterns of cdc2 expression in relation to meristem activity and cell proliferation during plant development. Proc Natl Acad Sci USA 89: 7360–7364 (1992).PubMedCrossRefGoogle Scholar
  93. 93.
    McClure BA, Guilfoyle T: Characterization of a class of small auxin-inducible soybean polyadenylated RNAs. Plant Mol Biol 9: 611–623 (1987).CrossRefGoogle Scholar
  94. 94.
    McGaw BA: Cytokinin biosynthesis and metabolism. In: Davies PJ (ed) Plant Hormones and their Role in Plant Growth and Development, pp. 76–93, Kluwer Academic Publishers, Dordrecht (1988).Google Scholar
  95. 95.
    Medford J, Horgan R, El-Sawi Z, Klee H: Alterations of endogenous cytokinins in transgenic plants using a chimeric isopentenyl transferase gene. Plant Cell 1: 403–413 (1989).PubMedGoogle Scholar
  96. 96.
    Miao G-H, Hong Z, Velma DPS: Two functional soybean genes encoding p34cdc2 protein kinases are regulated by different plant developmental pathways. Proc Natl Acad Sci USA 90: 943–947 (1993).PubMedCrossRefGoogle Scholar
  97. 97.
    Mitsui S, Wakasugi T, Sugiura M: A cDNA encoding the 57 kDa subunit of a cytokinin-binding protein complex trom tobacco -- the subunit has high homology to S-adenosyl-homocysteine hydrolase. Plant Cell Physiol 34: 1089–1096 (1993).Google Scholar
  98. 98.
    Mitsui S, Sugiura M: Purification and properties of a cytokinin-binding protein from tobacco leaves. Plant Cell Physiol 34: 543–547 (1993).Google Scholar
  99. 99.
    Mizoguchi T, Gotoh Y, Nishida E, Yamaguchi-Shinozaki K, Hayashida N, Iwasaki T, Kamada H, Shinozaki K: Characterization of two cDNAs that encode MAP kinase homologues in Arabidopsis thaliana and analysis of the possible role of auxin in activating such kinase activities in cultured cells. Plant J 5: 111–122 (1994).PubMedCrossRefGoogle Scholar
  100. 100.
    Moffatt B, Pethe C, Laloue M: Metabolism of benzyladenine is impaired in a mutant of Arabidopsis thaliana lacking adenosine phosphoribosyltransferase activity. Plant Physiol 95: 900–903 (1991).PubMedCrossRefGoogle Scholar
  101. 101.
    Moolenaar WH, Mummery CL, van der Saag PT, de Laat SW: Rapid ionic events and the initiation of growth in serum-stimulated neuroblastoma cells. Cell 23: 789–798 (1981).PubMedCrossRefGoogle Scholar
  102. 102.
    Muller J-F, Goujaud J, Caboche M: Isolation in vitro of naphthaleneacetic acid-tolerant mutants of Nitotiana tabacum which are impaired in root morphogenesis. Mol Gen Genet 199: 194–200 (1985).CrossRefGoogle Scholar
  103. 103.
    Napier RM, Venis MA: Monoclonal antibodies detect an auxin-induced conformational change in the maize auxin-binding protein. Planta 182: 313–318 (1990).CrossRefGoogle Scholar
  104. 104.
    Napier RM, Venis MA: Receptor for plant growth regulators: recent advances. J Plant Growth Regul 9: 113–126 (1990).CrossRefGoogle Scholar
  105. 105.
    Needleman P, Turk J, Jakschik B, Morrison AR, Lefkowith JB: Arachidonic acid metabolism. Annu Rev Biochem 55: 69–102 (1986).PubMedCrossRefGoogle Scholar
  106. 106.
    Ooms G. Kaup A, Roberts J: From tumor to tuber; tumor cell characteristics and chromosome numbers of crown gall derived tetraploid potato plants. Theor Appl Genet 66: 169–172 (1983).CrossRefGoogle Scholar
  107. 107.
    Pabo CT, Sauer R: Transcription factors: structural families and principles of DNA recognition. Annu Rev Biochem 61: 1053–1095 (1992).PubMedCrossRefGoogle Scholar
  108. 108.
    Palme K, Hesse T, Campos N, Garbers C, Yanofsky MF, Schell J: Molecular analysis of an auxin binding protein gene located on chromosome 4 of Arabidopsis. Plant Cell 4: 193–201 (1992).PubMedGoogle Scholar
  109. 109.
    Palme K: From binding proteins to hormone receptors? J Plant Growth Regul 12: 171–178 (1993).CrossRefGoogle Scholar
  110. 110.
    Palmgren MG, Sommarin M: Lysophosphatidylcholine stimulates ATP dependent proton accumulation in isolated oat root plasma membrane vesicles. Plant Physiol 90: 1009–1014 (1989).PubMedCrossRefGoogle Scholar
  111. 111.
    Park KY, Lee SH: Effects of ethylene and auxin on poly amine levels in suspension-cultured tobacco cells. Physiol Plant 90: 382–290 (1994).CrossRefGoogle Scholar
  112. 112.
    Parry AD, Blonstein AD, Babiano MJ, King PJ, Horgan R: Abscisic acid metabolism in a wilty mutant of Nicotiana plumbaginifolia Planta 183: 237–243 (1991).CrossRefGoogle Scholar
  113. 113.
    Perry CD, Cove DJ: Transfer RNA pool sizes and half lives in wild-type and cytokinin overproducing strains of the moss, Physcomitrella patens. Physiol Plant 67: 680–684 (1986).CrossRefGoogle Scholar
  114. 114.
    Poovaiah BW, Reddy ASN: Calcium and signal transduction in plants. Crit Rev Plant Sci 12: 185–211 (1993).PubMedGoogle Scholar
  115. 115.
    Ray PM: Auxin-binding sites of maize coleoptiles are localized on membranes of the endoplasmic reticulum. Plant Physiol 59: 594–599 (1977).PubMedCrossRefGoogle Scholar
  116. 116.
    Rayle DL, Cleland R: Control of plant cell enlargement by hydrogen ions. Curr Top Devel Biol 11: 187–214 (1977).CrossRefGoogle Scholar
  117. 117.
    Rayle DL, Cleland R: The acid growth theory of auxin-induced cell elongation is alive and well. Plant Physiol 99: 1271–1274 (1992).PubMedCrossRefGoogle Scholar
  118. 118.
    Reddy ASN, Poovaiah BW: Molecular cloning and sequencing of a cDNA for an auxin-repressed mRNA: correlation between fruit growth and repression of the auxin-regulated gene. Plant Mol Biol 14: 127–136(1990).PubMedCrossRefGoogle Scholar
  119. 119.
    Romano CP, Hein MB, Klee HJ: Inactivation of auxin in tobacco transformed with the indoleacetic acid-lysine synthetase gene of Pseudomonas savastanoi. Genes Devel 5: 438–446 (1991).PubMedCrossRefGoogle Scholar
  120. 120.
    Rousselin P, Kraepiel Y, Maldiney R, Miginiac E, Caboche M: Characterization of three hormone mutants of Nicotiana plumbaginifolia: evidence for a common ABA deficiency. Theor Appl Genet 85: 213–221 (1992).CrossRefGoogle Scholar
  121. 121.
    Rubery PH: Auxin receptors. Annu Rev Plant Physiol 32: 569–596(1981).CrossRefGoogle Scholar
  122. 122.
    Ruck A, Palme K, Venis MA, Napier RM, Felle HH: Patch-clamp analysis establishes a role for an auxin binding protein in the auxin stimulation of plasma membrane current in Zea mays protoplasts. Plant J 4: 41–46 (1993).CrossRefGoogle Scholar
  123. 123.
    Sakai S, Kikuchi M, Nakajima N: Interaction between auxin-binding protein-I and RNA polymerase II. Biosci Biotech Biochem 56: 1225–1229 (1992).CrossRefGoogle Scholar
  124. 124.
    Santoni V, Vansuyt, G, Rossignol, M: The changing sensitivity to auxin of the plasma-membrane H + -ATPase: relationship between plant development and ATPase content of membranes. Planta 185: 227–232 (1991).CrossRefGoogle Scholar
  125. 125.
    Schachtman DP, Schroeder JI, Lucas WJ, Anderson JA, Gaber RF: Expression of an inward-rectifying potassium channel by the Arabidopsis KAT1 cDNA. Science 258: 1654–1658 (1992).PubMedCrossRefGoogle Scholar
  126. 126.
    Schaefer D. Zyrd JP, Knight CD, Cove DJ: Stable transformation of the moss Physcomitrella patens. Mol Gen Genet 226: 418–424 (1991).PubMedGoogle Scholar
  127. 127.
    Schaller GE, Sussman, MR: Phosphorylation of the plasma-membrane H+ -ATPase of oat roots by a calcium stimulated protein kinase. Planta 173: 509–518 (1988).CrossRefGoogle Scholar
  128. 128.
    Scherer GFE, Andre B: Stimulation of phospholipase A2 by auxin in microsomes from suspension-cultured soybean cells is receptor-mediated and influenced by nucleotides. Planta 191: 515–523 (1993).CrossRefGoogle Scholar
  129. 129.
    Schmulling T, Beinsberger S, DeGreet J, Schell J, Van Onckelen H, Spena A: Construction of a heat inducible chimeric gene to increase cytokinin content in transgenic plant tissue. FEBS Lett 249: 401–406 (1989).CrossRefGoogle Scholar
  130. 130.
    Schmulling T, Fladung M, Grossman K, Schell J: Hormonal content and sensitivity of transgenic tobacco and potato plants expressing single rol genes of Agrobacterium rhizogenes T-DNA. Plant J 3: 371–382 (1993).CrossRefGoogle Scholar
  131. 131.
    Schmulling T, Schell J, Spena A: Single genes from Agrobacterium rhizogenes influence plant development. EMBO J 7: 2621–2629 (1988).PubMedGoogle Scholar
  132. 132.
    Schopfer P: Determination of auxin-dependent pH changes in coleoptile cell walls by a null-point method. Plant Physiol 103: 351–357 (1993).PubMedGoogle Scholar
  133. 133.
    Schwob E, Choi S-Y, Simmons, C, Migliaccio F, Hag L, Hesse T, Palme K, Soil D: Molecular analysis of three maize 22 kDa auxin-binding protein genes-transient promoter expression and regulatory regions. Plant J 4: 423–432 (1993).PubMedCrossRefGoogle Scholar
  134. 134.
    Sentenac H, Bonneaud N, Minet M, Lacroute F, Salmon J-M, Gaymard F, Glignon C: Cloning and expression in yeast of a plant potassium ion transport system. Science 256: 663–665 (1992).PubMedCrossRefGoogle Scholar
  135. 135.
    Shimomura S, Sotobayash T, Futai M, Fukui T: Purification and properties of an auxin-binding protein from maize shoot membranes. J Biochem 99: 1513–1524 (1986).PubMedGoogle Scholar
  136. 136.
    Shimomura S. Liu W, Inohara N, Watanabe S, Futai M: Structure of the gene for an auxin-binding protein and a gene for 7SL RNA from Arabidopsis thaliana. Plant Cell Physiol 34: 633–637 (1993).PubMedGoogle Scholar
  137. 137.
    Sitbon F, Ostin A. Sundberg B, Olsson O, Sandberg G: Conjugation of indole-3-acetic acid (IAA) in wild-type and IAA-overproducing transgenic tobacco plants, and identification of the main conjugates by frit-fast atom bombardment liquid chromatography-mass spectrometry. Plant Physiol 101: 313–320 (1993).PubMedGoogle Scholar
  138. 138.
    Skoog F, Miller C: Chemical regulation of growth and organ formation in plant tissues cultured in vitro. Soc Expt Biol Symp 11: 188–231 (1957).Google Scholar
  139. 139.
    Smart CM, Scofield SR, Bevan MW, Deyer TA: Delayed leaf senescence in tobacco plants transformed with tmr, a gene for cytokinin production in Agrobacterium. Plant Cell 3: 647–656 (1991).PubMedGoogle Scholar
  140. 140.
    Smigocki AC: Cytokinin content and tissue distribution in plants transformed by a reconstructed isopentenyl transferase gene. Plant Mol Biol 16: 105–115 (1991).PubMedCrossRefGoogle Scholar
  141. 141.
    Smigocki AC, Owens LD: Cytokinin to auxin ratios and morphology of shoots and tissues transformed by chimeric isopentenyltransferase gene. Plant Physiol 91: 808–811(1989).PubMedCrossRefGoogle Scholar
  142. 142.
    Spena A, Prinsen E, Fladung M, Schulze SC, Van Onckelen H: The indoleacetic acid-lysine synthetase gene of Pseudomonas syringae subsp. savastanoi induces developmental alterations in transgenic tobacco and potato plants. Mol Gen Genet 227: 205–212 (1991).PubMedCrossRefGoogle Scholar
  143. 143.
    Su W, Howell S: A single genetic locus, CKR1, defines Arabidopsis mutants in which root growth is resistant to low concentrations of cytokinin. Plant Physiol 99:1569–1574 (1992).PubMedCrossRefGoogle Scholar
  144. 144.
    Takahashi Y, Nagata T: pal B: An auxin-regulated gene encoding glutathiones-transferase. Proc Natl Acad Sci USA 89: 56–59 (1992).PubMedCrossRefGoogle Scholar
  145. 145.
    Theologis A, Huynh TV, Davis RW: Rapid induction of specific mRNAs by auxin in pea epicotyl tissue. J Mol Biol 183: 53–68 (1985).PubMedCrossRefGoogle Scholar
  146. 146.
    Thiel G, Blatt MR, Fricker MD, White IR, Millner P: Modulation of K + -channels in Vicia stomatal guard cells by peptide homologues to the auxin-binding protein C terminus. Proc Natl Acad Sci USA 90: 1493–1497 (1993).CrossRefGoogle Scholar
  147. 147.
    Tillmann U, Viola G, Kayser B, Siemeister G, Hesse T, Palme K, Lobler M, Klambt D: cDNA clones of the auxin-binding protein from com coleoptiles (Zea mays L.): isolation and characterization by immunological methods. EMBO J 8: 2463–2467 (1989).PubMedGoogle Scholar
  148. 148.
    Timmerman KP: Molecular characterization of corn glutathione-S-transferase isozymes involved in herbicide detoxification. Physiol Plant 77: 465–471 (1989).CrossRefGoogle Scholar
  149. 149.
    Tretyn, A, Wagner G, Felle HH: Signal transduction in Sinapsis alba root hairs: auxins as external messengers. J Plant Physiol 139: 187–193 (1991).CrossRefGoogle Scholar
  150. 150.
    Venis MA, Thomas EW, Barbier-Brygoo H, Ephritikhine G, Guem J: Impermeant auxin analogues have auxin activity. Planta 182: 232–235 (1990).CrossRefGoogle Scholar
  151. 151.
    Venis MA, Napier RM, Barbier-Brygoo H, Maurel C, Perrot-Rechenmann C, Guem J: Antibodies to a peptide from the maize auxin-binding protein have auxin agonist activity. Proc Natl Acad Sci USA 98: 7208–7212 (1992).CrossRefGoogle Scholar
  152. 152.
    Verhey SD, Lomax TL: Signal transduction in vascular plants. J Plant Growth Regul 12: 179–195 (1993).CrossRefGoogle Scholar
  153. 153.
    Vesper MJ, Kuss CL: Physiological evidence that the primary site of auxin action is an intracellular site. Planta 182: 486–491 (1991).CrossRefGoogle Scholar
  154. 154.
    Waring PF, Phillips IDJ: Growth and differentiation in plants. Pergamon Press, New York (1981).Google Scholar
  155. 155.
    Walker JC, Key JL: Isolation of cloned cDNAs to auxin-responsive poly(A)+ RNAs of elongating soybean hypocotyl. Proc Natl Acad Sci USA 9: 7185–7189 (1982).CrossRefGoogle Scholar
  156. 156.
    Wang TL, Horgan R, Cove D: Cytokinins from the moss Physcomitrella patens. Plant Physiol 68: 735–738 (1981).PubMedCrossRefGoogle Scholar
  157. 157.
    Wang TL, Beutelmann P, Cove D: Cytokinin biosynthesis in mutants of the moss Physcomitlella patens. Plant Physiol 68: 739–744 (1981).PubMedCrossRefGoogle Scholar
  158. 158.
    Wang TL, Futers TS, McGeory F, Cove DJ: Moss mutants and the analysis of cytokinin metabolism. In: Crozier A, Hillman JR (eds) The Biosynthesis and Metabolism of Plant Hormones. Society of Experimental Biologists Seminar Series 23, pp. 135–164, Cambridge University Press, Cambridge (1984).Google Scholar
  159. 159.
    Yamamoto KT, Mori H, Imaseki H: cDNA cloning of indole-3-acetic acid-regulated genes: Aux22 and SAUR from mung bean (Vigna radiata) hypocotyl tissue. Plant Cell Physiol 33: 93–97 (1992).Google Scholar
  160. 160.
    Yamamoto KT: Further characterization of auxin-regulated mRNAs in hypocotyl sections of mung bean (Vigna radiata (L.) Wilczek): Sequence homology to genes for fatty-acid desaturases and atypical late-embryogenesis-abundant protein, and the mode of expression of the mRNAs. Planta 192: 359–364 (1994).PubMedCrossRefGoogle Scholar
  161. 161.
    Yang T, Law DM, Davies PJ: Magnitude and kinetics of stem elongation induced by exogenous indole-3-acetic acid in intact light-growth pea seedlings. Plant Physiol 102: 717–724 (1993).PubMedGoogle Scholar
  162. 162.
    Yu L-X, Lazarus CM: Structure and sequence of an auxin-binding protein gene from maize (Zea mays L.). Plant Mol Biol 16: 925–930 (1991).PubMedCrossRefGoogle Scholar
  163. 163.
    Zbell B, Walter-Back C: Signal transduction of auxin on isolated plant cell membranes: indications for a rapid polyphosphoinositide response stimulated by indoleacetic acid. J Plant Physiol 133: 353–360 (1988).CrossRefGoogle Scholar
  164. 164.
    Zettl R. Schell J, Palme K: Photoaffinity labeling of Arabidopsis thaliana plasma membrane vesicles by 5-azido-[7–3H]indole-3-acetic acid: Identification of a glutathione S-transferase. Proc Natl Acad Sci USA 91: 689–693 (1994).PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 1994

Authors and Affiliations

  • Lawrence Hobbie
    • 1
  • Candace Timpte
    • 1
  • Mark Estelle
    • 1
  1. 1.Department of BiologyIndiana UniversityBloomingtonUSA

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