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Inflammation: Mechanisms and Treatment pp 253–257Cite as

The possible role of divalent cations, microtubules and cyclic nucleotides in lymphokine induced macrophage aggregation

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Part of the book series: Inflammation: Mechanisms and Treatment ((FTIN,volume 4))

Abstract

Lymphokines (LKY) are non-antibody-soluble products of activated lymphocytes, thought to be mediators of cell mediated immunity. They include putative inflammatory mediators of delayed hypersensitivity such as skin reactive factor. Although they have a number of in vivoand in vitroeffects, the interaction of LKY with macrophages as their target cell has been best studied. The ability of migration inhibitory factor (MIF) reversibly to decrease the spontaneous migration of macrophages in vitrois the basis of the most widely used assay system for lymphokine activity. Despite much work the exact molecular mechanism of MIF action is not known, although recent work is encouraging. It has been known for some time that MIF containing lymphocyte culture supernatants will aggregate macrophages; this was first studied systematically by Lolekha et al 1. These authors showed that macrophage aggregating factor (MAF) was produced by activated lymphocytes under the same conditions as was MIF. Both the aggregation of normal macrophages by preformed LKY (indirect) or the aggregation of sensitized PEC (peritoneal exudate cells, containing both macrophages and lymphocytes) by specific antigen (direct) was observed. The production of MAF and the direct aggregation of sensitized PEC was found to correlate with the state of delayed hypersensitivity in the guinea pig.

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References

  1. Lolekha, S., Dray, S. and Gotoff, S. P. (1970). Macrophage aggregation in vitro:a correlate with delayed hypersensitivity. J. Immunol., 104, 296

    PubMed  CAS  Google Scholar 

  2. Rouveix, B., Badenoch-Jones, P. and Turk, J. L. (1979). A sensitive technique for measuring specific macrophage aggregation: a comparison with macrophage migration inhibition, for the detection of lymphokine activity. Immunology, 36, 589

    PubMed  CAS  Google Scholar 

  3. Badenoch-Jones, P., Rouveix, B. and Turk, J. L. (1980). Studies on lymphokine-induced macrophage aggregation. Specificity and quantitative aspects. Int. Arch. Allergy Appl. Immunol.(In press)

    Google Scholar 

  4. Pick, E. and Abrahamer, H. (1973). Blocking of macrophage migration inhibitory action by microtubular disruptive drugs. Int. Arch. Allergy Appl. Immunol., 44, 215

    Article  PubMed  CAS  Google Scholar 

  5. Pick, E., Honig, S. and Griffel, B. (1979). The mechanism of action of soluble lymphocyte mediators. VI. Effect of MIF on macrophage microtubules. Int. Arch. Allergy Appl. Immunol., 62, 188

    Google Scholar 

  6. McCarthy, P. L., Shaw, J. E. and Remold, H. G. (1979). The role of microtubules in the response of macrophages to MIF. Cell Immunol., 46, 409

    Article  PubMed  CAS  Google Scholar 

  7. Snyder, J. A. and Mcintosh, J. R. (1976). Biochemistry and physiology of microtubules. Ann. Rev. Biochem., 45, 699

    Article  PubMed  CAS  Google Scholar 

  8. Badenoch-Jones, P., Rouveix, B. and Turk, J. L. (1979). Studies on the mechanism of macrophage aggregation induced by lymphokines. Immunology, 37, 361

    PubMed  CAS  Google Scholar 

  9. Block, L. H., Aloni, B., Blemesderfer, D., Kashgarian, M. and Bitensky, M. W. (1978). Macrophage migration inhibition factor: interactions with calcium, magnesium and cyclic AMP. J. Immunol, 121, 1416

    PubMed  CAS  Google Scholar 

  10. Hand, L. W., King, N. L., Johnson, J. D. and Lowe, D. A. (1977). Requirement for magnesium influx in activation of alveolar macrophages mediated by ionophore A23187. Nature (London), 265, 543

    Article  CAS  Google Scholar 

  11. Greindeder, D. K. and David, J. R. (1977). The role of calciums in the action of MIF induced by neuraminidase-galactose oxidase. Fed. Proc, Fed. Am. Soc. Exp. Biol., 36, 1218

    Google Scholar 

  12. Oliver, J. M., Zuvier, R. B. and Berlin, R.D. (1975). Concanavalin A Cap formation on polymorphonuclear leukocytes of normal and beige mice. Nature (London), 263, 471

    Article  Google Scholar 

  13. Bendtzen, K. and Klysner, R. (1979). Increased polymorphonuclear leukocyte cGMP levels induced by the human lymphokine, leukocyte migration inhibitory factor (LIF). Immuno-pharmacology, 1, 323

    Article  CAS  Google Scholar 

  14. Pick, E. and Manheimer, S. (1974). The mechanism of action of soluble lymphocyte mediators. II. Modification of macrophage migration and MIF action by drugs, enzymes and cationic environment. Cell Immunol., 11, 30

    Article  PubMed  CAS  Google Scholar 

  15. Rouveix, B., Badenoch-Jones, P., Larne, S. and Turk, J. L. (1980). Lymphokine-induced macrophage aggregation: the role of cyclic nucleotides. (In preparation)

    Google Scholar 

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Author information

Authors and Affiliations

  1. UK

    P. Badenoch-Jones & J. L. Turk

  2. France

    B. Rouveix

Authors
  1. P. Badenoch-Jones
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  2. B. Rouveix
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  3. J. L. Turk
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Editor information

Editors and Affiliations

  1. Department of Experimental Pathology and Rheumatology, St Bartholomew’s Hospital, London, UK

    D. A. Willoughby (Professor) (Professor)

  2. Department of Pharmacology, Hôpital Cochin, Paris, France

    J. P. Giroud (Professor) (Professor)

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© 1980 MTP Press Limited

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Badenoch-Jones, P., Rouveix, B., Turk, J.L. (1980). The possible role of divalent cations, microtubules and cyclic nucleotides in lymphokine induced macrophage aggregation. In: Willoughby, D.A., Giroud, J.P. (eds) Inflammation: Mechanisms and Treatment. Inflammation: Mechanisms and Treatment, vol 4. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-9423-8_37

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  • DOI: https://doi.org/10.1007/978-94-010-9423-8_37

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-9425-2

  • Online ISBN: 978-94-010-9423-8

  • eBook Packages: Springer Book Archive

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