Summary
It is shown that the determination of fluorescence decay at various emission wavelengths enables us to resolve the fluorescence spectra into several components.
The method is applied to the study of the endonuclease of Staphylococcus aureus fluorescence and of the human serum albumin emission. In the first case, one can decompose the spectrum into two components each of them being characterized by one decay time: the tyrosine emission with a decay time of 1.8 nsec and the tryptophan emission with a decay time of 5.5 nsec. In the second case, the spectrum is decomposed into three components: the tyrosine emission (1.5 nsec), the tryptophan emission (6.17 nsec) and a third component which has a weak contribution and which is ascribed to an other kind of tryptophan emission (decay time 12.08 nsec).
Résumé
On montre que la mesure des déclins de fluorescence à différentes longueurs d’onde d’émission peut permettre de résoudre les spectres de fluorescence en plusieurs composantes.
La méthode a été appliquée à l’émission de fluorescence de l’endonucléase du Staphylococcus aureus et de la sérum albumine humaine. Dans le premier cas, la méthode permet de décomposer le spectre en deux composantes caractérisées chacune par une constante de temps. Il s’agit de la tyrosine de constante de temps de 1,8 nsec et du tryptophane (5,5 nsec). Dans le second cas, le spectre est décomposé en trois composantes: la tyrosine (1,5 nsec), le tryptophane (6,17 nsec) et une troisième composante en faible proportion attribuée également au tryptophane (12,08 nsec).
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© 1973 D. Reidel Publishing Company, Dordrecht, Holland
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Wahl, P., Brochon, J.C. (1973). Resolution of the Fluorescence Spectra of Proteins Using Decay Measurements. In: Sadron, C. (eds) Dynamic Aspects of Conformation Changes in Biological Macromolecules. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-2579-9_8
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DOI: https://doi.org/10.1007/978-94-010-2579-9_8
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